LOCUS Exported 3370 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3370 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1408..1455 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3303..3319 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 3320..3351 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1336 /label="Mfl325" /note="" /translation="MKKLLAILAATALITPVVTSVISCGENQEPFTNNPLMIGKGIDKSKAIDDSQPGKFGFTNFYIVGDSLSDTDGISNLIKTKFANQMVDLNVSLSGAYGYEKNDVHYSAFSNGMTAGTILSEKLGFGEMKPSNFLSKKESTNYGKNYSVGGATAAKLDLPTGILLNDATIDRQTEALLSQHKINNNDLVFFEIGGNDLFSLIGFEGNEAKQIEFMNDSIDRVRTALFNLLNNGIKNIIFMTPPRMDFPPRYRDIFNEANAGNSKAKKTAEFIINICDEYYSKIMNVLNEVEQYYPDSIELYDLYKKTNELENEFKKIAGQDAIIDKAYSDGQAIEIKLNNEKMTFNTDPKLDLFDNVSSVINEFKSDIVFGAKNTLDITISATRSTSRSLYNNNERDKDMSKFFFTDFVHPTKQVHELVSEILLDHAKELSKKWEN" primer_bind complement(1460..1476) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1543..2131) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2322..3182) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(3183..3287) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAAGCACTCT TAGGCCTCTG GGTCTCAAAT GAAGAAACTG CTGGCGATTC TGGCGGCGAC 61 TGCGCTGATC ACGCCGGTCG TTACGAGCGT GATTAGCTGC GGTGAGAACC AAGAGCCTTT 121 CACCAACAAC CCATTGATGA TTGGCAAAGG CATCGACAAA TCCAAAGCGA TCGATGATTC 181 CCAGCCGGGT AAATTCGGTT TTACCAATTT TTATATCGTT GGCGATAGCC TGAGCGACAC 241 CGATGGCATC AGCAATCTGA TCAAGACCAA ATTCGCAAAT CAGATGGTTG ATCTGAACGT 301 TTCGCTGAGC GGTGCTTACG GCTACGAGAA GAACGATGTT CACTATAGCG CGTTCAGCAA 361 TGGTATGACC GCGGGTACTA TTCTGAGCGA AAAACTGGGC TTCGGTGAAA TGAAACCGAG 421 CAATTTTCTG AGCAAGAAGG AGTCCACCAA TTATGGTAAA AATTATAGCG TCGGTGGCGC 481 GACGGCCGCG AAATTAGATC TGCCGACGGG CATCCTGCTG AACGACGCAA CTATCGACCG 541 CCAAACCGAG GCACTGCTGT CCCAGCACAA GATCAACAAT AACGATCTGG TGTTTTTCGA 601 AATCGGTGGC AATGATCTGT TCAGCCTGAT TGGTTTCGAA GGTAACGAAG CCAAGCAAAT 661 CGAGTTCATG AATGATTCAA TCGATCGCGT CCGCACCGCT CTGTTTAATC TGCTGAACAA 721 CGGTATTAAA AATATTATCT TTATGACGCC GCCGCGCATG GACTTCCCTC CGCGTTACCG 781 TGACATCTTC AACGAAGCCA ACGCGGGTAA TAGCAAGGCG AAGAAAACCG CGGAGTTCAT 841 TATCAATATC TGCGACGAGT ACTATAGCAA AATCATGAAC GTTCTGAACG AAGTTGAGCA 901 ATATTATCCA GATTCGATTG AACTGTACGA TCTGTATAAG AAAACCAATG AACTGGAGAA 961 TGAGTTTAAG AAAATTGCGG GCCAGGATGC CATCATTGAT AAAGCATACT CTGATGGCCA 1021 GGCGATTGAG ATTAAATTGA ATAATGAAAA GATGACTTTT AATACGGACC CGAAGCTGGA 1081 CTTGTTCGAC AATGTCAGCA GCGTGATCAA CGAGTTTAAG AGCGACATCG TGTTTGGCGC 1141 AAAGAACACC CTCGATATCA CCATTAGCGC GACGCGTTCC ACCAGCCGCA GCCTGTATAA 1201 TAATAATGAG CGTGACAAGG ACATGAGCAA GTTTTTCTTC ACCGACTTTG TTCACCCGAC 1261 GAAACAGGTC CACGAACTGG TCAGCGAAAT TTTGCTGGAC CATGCGAAAG AGCTGAGCAA 1321 GAAATGGGAG AACTGAAGAG CTTAGAGACC CCGGTTGTAC CTATCGAGTG CGCTGAGGTG 1381 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1441 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1501 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1561 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1621 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1681 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1741 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1801 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1861 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1921 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1981 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 2041 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 2101 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 2161 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 2221 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 2281 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 2341 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 2401 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 2461 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 2521 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 2581 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 2641 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 2701 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 2761 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 2821 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 2881 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 2941 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 3001 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 3061 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 3121 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 3181 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 3241 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 3301 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT 3361 GGAGTGGGAG //