LOCUS       Exported     3277 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3277
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1315..1362
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3210..3226
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3227..3258
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1243
                     /label="Mfl331"
                     /note="Bidirectionally degrades single-stranded DNA into large acid-insoluble oligonucleotides, which are then degraded further into small acid-soluble oligonucleotides."
                     /translation="MENKIFSVAEISQIFKEAIEGSNYFKNIYVRGEVGNLTFNKSGHVYFSLKDNQSTIAAMIWKSNAHKLTNLNVKEGMEITCYGRLTYYVPSGRVSFEAVDVSVEGKGDLQEIFEERLKEITALGWTDESRKKPINRFAKNIGLITTDSGAAIKDLITTLKRRMPSVNIYLFPTMVQGETAKFDIANKIQQANLFEPKLDILIVGRGGGSYEDLWTFNEMKVLKAIVDSSIPVISAVGHEPDITLSDYVADLRAATPTAAAELASISTEELLKELAYNYENQIRLFKNVYNNQQLKIEEFKKQNILKINNKYDLQSLDLNYIEKSFINNINNIISRNEMFIENIESKTALLDPKKPLDKGFGLIMSKDKQIINSVDKVDINQEIKLVLKDGEIETIIKGVKKHGK"
     primer_bind     complement(1367..1383)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1450..2038)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2229..3089)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3090..3194)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GGAAAACAAG ATTTTCTCTG TTGCAGAAAT
       61 CTCTCAAATC TTTAAAGAGG CCATCGAAGG CTCCAATTAC TTCAAGAATA TTTATGTGCG
      121 CGGTGAGGTT GGCAACCTGA CGTTCAATAA AAGCGGCCAC GTGTATTTCA GCCTGAAGGA
      181 CAATCAAAGC ACGATCGCCG CCATGATCTG GAAAAGCAAC GCCCACAAAC TGACTAATCT
      241 GAACGTCAAA GAGGGCATGG AAATTACGTG CTACGGCCGC CTGACCTATT ACGTTCCGAG
      301 CGGTCGTGTC AGCTTTGAGG CAGTAGATGT GTCTGTGGAA GGTAAGGGCG ATCTGCAAGA
      361 AATCTTTGAG GAGCGTCTGA AAGAGATTAC CGCCCTCGGT TGGACGGATG AGTCGCGTAA
      421 AAAACCGATC AACCGTTTCG CGAAAAATAT CGGCCTGATT ACGACGGATA GCGGTGCAGC
      481 TATTAAAGAC CTGATCACGA CCCTGAAGCG TCGTATGCCG AGCGTGAATA TTTATCTGTT
      541 TCCGACCATG GTCCAAGGTG AAACCGCGAA GTTCGATATC GCGAATAAAA TTCAGCAAGC
      601 GAACTTGTTT GAGCCGAAGC TGGATATTCT GATCGTGGGC CGCGGTGGTG GCAGCTACGA
      661 AGATCTGTGG ACGTTTAATG AGATGAAAGT GCTGAAAGCC ATTGTCGATT CCAGCATTCC
      721 GGTGATTAGC GCGGTTGGCC ACGAGCCGGA CATTACTCTG AGCGACTATG TGGCTGACTT
      781 GCGTGCCGCT ACTCCGACCG CGGCGGCAGA ACTGGCTTCC ATTTCCACCG AAGAATTGCT
      841 GAAGGAACTG GCCTACAATT ACGAAAATCA AATTCGTCTG TTCAAAAACG TTTATAACAA
      901 CCAACAGCTG AAAATCGAAG AGTTTAAGAA ACAGAACATT CTGAAGATTA ATAATAAATA
      961 CGATCTGCAA TCTCTGGACC TGAACTACAT TGAAAAGAGC TTTATCAATA ACATTAACAA
     1021 CATTATCTCT CGTAATGAGA TGTTCATCGA AAACATTGAA AGCAAGACCG CGCTGCTGGA
     1081 TCCGAAGAAA CCGCTGGATA AAGGCTTCGG TCTGATCATG AGCAAGGACA AACAAATTAT
     1141 CAATAGCGTT GACAAAGTGG ACATCAACCA GGAAATCAAG CTGGTTTTGA AGGACGGCGA
     1201 GATCGAAACG ATCATCAAGG GTGTGAAAAA GCACGGTAAG TGAAGAGCTT AGAGACCCAT
     1261 CCTTATGCGA TACGGGTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA
     1321 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC
     1381 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA
     1441 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     1501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     1561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     1621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     1681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     1741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     1801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     1861 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     1921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     1981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2041 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
     2101 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
     2161 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
     2221 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
     2281 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
     2341 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA
     2401 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
     2461 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
     2521 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
     2581 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
     2641 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
     2701 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
     2761 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
     2821 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
     2881 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
     2941 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
     3001 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
     3061 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT
     3121 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA
     3181 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC
     3241 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG
//