LOCUS       Exported     2944 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2944
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      982..1029
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2877..2893
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2894..2925
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..910
                     /label="Mfl333"
                     /note="Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic (AP) sites, generating a 3'-hydroxyl group and a 5'-terminal sugar phosphate."
                     /translation="MKKPILGSHVGMSKSNKHGEYLIGSVKEALSYEANTLMFYTGAPQNSVRTATDKLYIEEFNQLLKENNINKDHIVVHAPYIINISNPVNQTTWDFGVDFLKQEIKRCEDIGVKILVLHPGARLKGDYTDALNALVKGLNAVASSQTNVIIALETMAGKGTEVGLSLNDFKYVIDNVNEPEKVAVCLDTCHMHDAGYDFNNWELIKEEINKTIGKDKVLCFHLNDSKNPLLAHKDRHENIGYGYIGFDNLLKVLWDEEYIDIPKILETPYVEDKPPYKEEIENLINKNFSKKVK"
     primer_bind     complement(1034..1050)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1117..1705)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1896..2756)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2757..2861)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAAGAAGCCG ATTCTGGGTA GCCATGTGGG
       61 CATGAGCAAA TCAAATAAGC ACGGTGAGTA TCTGATCGGT AGCGTGAAGG AAGCACTGAG
      121 CTATGAGGCC AATACGCTGA TGTTTTACAC CGGCGCGCCG CAGAACAGCG TTCGCACGGC
      181 GACGGACAAA CTGTACATTG AGGAGTTCAA TCAACTGCTG AAAGAAAATA ACATCAATAA
      241 GGATCACATC GTTGTCCACG CACCGTATAT CATCAATATT AGCAACCCGG TCAACCAAAC
      301 TACGTGGGAC TTCGGTGTCG ACTTCCTGAA ACAGGAGATT AAACGCTGTG AGGATATTGG
      361 TGTTAAAATC TTGGTTCTGC ACCCGGGTGC GCGCCTGAAA GGTGACTATA CCGACGCCTT
      421 GAATGCGTTG GTGAAGGGCC TGAATGCGGT GGCGAGCAGC CAAACGAACG TCATCATTGC
      481 ACTGGAAACC ATGGCTGGTA AAGGTACGGA AGTTGGTTTG AGCCTGAATG ACTTTAAGTA
      541 CGTGATCGAC AATGTGAACG AGCCGGAAAA AGTTGCGGTC TGCCTGGACA CGTGCCATAT
      601 GCACGACGCC GGTTATGATT TCAATAACTG GGAGCTGATC AAAGAGGAGA TCAATAAAAC
      661 CATCGGCAAA GACAAGGTGC TGTGCTTTCA TCTGAACGAT AGCAAGAACC CTTTATTGGC
      721 CCACAAGGAC CGTCACGAGA ATATTGGTTA CGGCTATATC GGTTTTGATA ATCTGCTGAA
      781 AGTTCTGTGG GACGAAGAAT ATATCGACAT CCCGAAAATT CTCGAAACCC CGTACGTTGA
      841 GGATAAACCG CCGTATAAAG AGGAAATCGA AAACCTGATC AACAAGAATT TCAGCAAGAA
      901 GGTAAAGTGA AGAGCTTAGA GACCGCCACG GCGAAATTTA ATGTCGCTGA GGTGTCAATC
      961 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     1021 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     1081 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1141 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1201 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1261 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1321 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1381 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1441 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1501 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1561 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1621 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1681 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1741 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1801 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     1861 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     1921 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     1981 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     2041 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2101 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2161 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2221 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2281 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2341 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2401 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2461 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2521 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2581 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2641 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2701 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2761 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     2821 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     2881 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     2941 GGAG
//