LOCUS Exported 3196 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3196 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1234..1281 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3129..3145 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3146..3177 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1162 /label="Mfl339" /note="" /translation="MKTLLSVLGAIGLSATAASPIISLSTEENIKDIKNDKVNLEYGTNSLTPYDFSDNYLRRATTMNKFSGRGVETVESNFTVPLPNAITVNDILNDSKIDTINYGGIFNIEAASVNQRFTAILLKRDPSQNTKDKAVFMGNIHQNNSATSIGWSLDLELTYYIDSKGNAFADIKLNQTVVDGYRYTNSLTGEVGVKIFFKTERTHYYINNTVSTEQDMGTELLATIFNISSSNSAVVTTKDRVINQSISGLTGSTVSTQGINLQNTKRVSFYGTMRRSIQQAGYNSNIMLDSIDFIKNEELSTEYNLVFETNYETIGINESTGNWNSSLNLTLNINIDPATNRVTLSNKGIIDLDNSETTNANTWMETTWVFDRASLWG" primer_bind complement(1286..1302) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1369..1957) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2148..3008) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3009..3113) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GAAAACTCTG TTGAGCGTCT TGGGCGCCAT 61 CGGCCTGAGC GCCACCGCGG CAAGCCCTAT TATTAGCCTG AGCACGGAGG AGAATATCAA 121 GGACATCAAG AACGATAAAG TTAACCTGGA GTACGGTACG AACTCCCTCA CGCCTTATGA 181 CTTTAGCGAC AATTATCTGC GTCGCGCTAC GACCATGAAC AAATTTAGCG GCCGTGGCGT 241 GGAAACCGTC GAGAGCAATT TTACGGTTCC GTTACCGAAC GCCATCACGG TCAATGACAT 301 TCTGAACGAC AGCAAGATTG ATACTATTAA CTATGGCGGC ATCTTTAACA TCGAGGCGGC 361 CTCGGTTAAT CAACGTTTCA CCGCAATCCT GCTGAAACGT GATCCGAGCC AAAATACTAA 421 AGATAAGGCC GTGTTTATGG GCAACATCCA CCAGAACAAC AGCGCGACCA GCATTGGTTG 481 GAGCCTGGAT TTGGAACTGA CGTATTACAT CGACAGCAAG GGTAATGCCT TTGCCGATAT 541 TAAGCTGAAT CAGACCGTTG TGGATGGTTA CCGTTACACG AACAGCCTGA CGGGCGAAGT 601 GGGTGTTAAG ATCTTTTTTA AAACGGAGCG TACGCATTAC TACATCAACA ACACCGTTAG 661 CACCGAGCAG GATATGGGTA CCGAACTGCT GGCAACCATC TTCAATATCT CGAGCAGCAA 721 CAGCGCGGTC GTCACGACTA AAGATCGTGT TATCAACCAG AGCATCAGCG GTCTGACCGG 781 TAGCACCGTT AGCACCCAGG GCATCAATCT GCAAAACACC AAGCGTGTTA GCTTTTACGG 841 TACCATGCGT CGCTCCATCC AACAAGCGGG CTATAATTCC AACATTATGC TGGATAGCAT 901 TGATTTCATT AAGAATGAGG AGCTGAGCAC CGAATATAAT TTGGTGTTTG AAACCAATTA 961 TGAAACGATT GGCATCAATG AAAGCACCGG TAATTGGAAC TCCTCGCTGA ATCTGACTCT 1021 GAATATTAAC ATCGACCCTG CCACCAACCG TGTGACTCTG TCCAATAAAG GCATTATTGA 1081 TCTGGATAAT AGCGAAACCA CGAATGCGAA TACGTGGATG GAAACGACGT GGGTTTTTGA 1141 TCGCGCCAGC CTGTGGGGCT GAAGAGCTTA GAGACCCGGA TTCTAGGTGC AACTCTCGCT 1201 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA 1261 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG 1321 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT 1381 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 1441 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 1501 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 1561 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 1621 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 1681 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 1741 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 1801 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 1861 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 1921 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC 1981 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG 2041 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA 2101 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA 2161 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC 2221 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG 2281 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA 2341 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT 2401 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT 2461 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC 2521 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC 2581 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA 2641 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG 2701 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG 2761 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA 2821 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA 2881 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA 2941 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA 3001 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 3061 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC 3121 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG 3181 TCAGGTGGAG TGGGAG //