LOCUS       Exported     3160 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3160
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1198..1245
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3093..3109
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3110..3141
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1126
                     /label="Mfl351"
                     /note=""
                     /translation="MSKCIGCGILKQNKDQNKPGYVIKPENEYCLRCFKIKNYNELINQEINAEQFILKLKTLIQNDKPKDIEFFYIIDIFDLEGSRVKEIEEAIKRYPVTIVVNKIDLLPKAVKLAKIRRYITDLFAKSAIYDANIILNTSFKNNFTNSLLNKIKKTKTKKYFIGSSNVGKSSIINSLLRTNNLIPQIVESKYFNTTLDFIQIKLSSDDIIFDTPGIARSNSAANLLDKEDWKYIYFNKEVAQNTFQLKPFNSIFYAGIAWVDFEWESDEINSFHFFNNKEIKLHRTNTKNAFDYYTRNKENIVPYNLDNNFVKREFIFNEKDINKEFEISISGLGWFNFKVKNPMKVTINIPTSNVEVETTLRKPLV"
     primer_bind     complement(1250..1266)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1333..1921)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2112..2972)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2973..3077)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GAGCAAGTGT ATCGGTTGCG GCATCCTGAA
       61 ACAAAACAAA GACCAAAACA AGCCGGGTTA CGTCATTAAG CCGGAAAATG AATACTGTCT
      121 GCGCTGCTTT AAGATCAAGA ACTACAATGA ACTGATCAAC CAGGAGATTA ATGCTGAGCA
      181 ATTTATTCTG AAGCTGAAAA CCTTGATTCA GAATGACAAG CCTAAGGACA TTGAGTTCTT
      241 CTACATCATC GATATCTTTG ATCTGGAAGG TAGCCGTGTG AAGGAAATCG AAGAGGCGAT
      301 CAAGAGATAT CCTGTAACGA TCGTCGTCAA CAAAATCGAT CTGCTCCCAA AAGCCGTTAA
      361 ATTGGCGAAA ATTAGACGCT ACATCACCGA TTTATTTGCT AAGTCCGCGA TCTATGACGC
      421 AAATATCATT CTGAACACGA GCTTTAAGAA CAATTTTACG AATTCACTGC TGAACAAGAT
      481 TAAAAAGACG AAAACGAAAA AATACTTCAT CGGTAGCAGC AATGTGGGTA AGAGCTCAAT
      541 CATCAATAGC CTGCTGCGTA CGAATAATCT GATTCCACAA ATCGTTGAGA GCAAATACTT
      601 TAACACGACC TTAGACTTTA TTCAGATTAA GCTGTCTAGC GACGACATTA TTTTCGATAC
      661 CCCGGGTATC GCCCGTAGCA ACAGCGCGGC TAATCTGTTG GACAAGGAGG ACTGGAAATA
      721 CATCTATTTT AATAAAGAGG TCGCCCAGAA TACTTTCCAG CTGAAGCCGT TTAATAGCAT
      781 TTTTTATGCG GGTATCGCGT GGGTAGACTT CGAATGGGAG AGCGACGAGA TCAACTCCTT
      841 CCACTTCTTC AACAATAAGG AAATCAAACT GCACCGTACT AATACGAAAA ATGCATTTGA
      901 CTACTATACC CGTAATAAAG AGAATATCGT TCCGTACAAC CTGGACAATA ATTTTGTTAA
      961 GCGCGAGTTT ATCTTTAATG AGAAGGACAT TAATAAAGAA TTTGAGATCA GCATCTCCGG
     1021 TTTGGGTTGG TTCAATTTCA AGGTCAAAAA TCCGATGAAA GTGACGATCA ACATTCCGAC
     1081 CAGCAACGTT GAGGTTGAAA CGACCCTGCG CAAGCCGCTG GTGTGAAGAG CTTAGAGACC
     1141 GGGCACTCAA TATACCCTGT CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA
     1201 GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT
     1261 TTCCTGAGAG CTTGGCAGGT GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA
     1321 GAATGCCATT AATTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
     1381 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
     1441 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
     1501 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
     1561 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
     1621 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
     1681 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
     1741 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
     1801 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
     1861 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
     1921 AGAAGGCCTA CTATTAGCAA CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG
     1981 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC
     2041 TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT
     2101 TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT
     2161 CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA
     2221 CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA
     2281 TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC
     2341 GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT
     2401 AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT
     2461 ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG
     2521 TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA
     2581 GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA
     2641 AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG
     2701 CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT
     2761 TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG
     2821 CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT
     2881 ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA
     2941 ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC
     3001 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA
     3061 CAAATAGGGG TTCCGCGCAC CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC
     3121 TCCGACCGGA GGCTTTTGAC TTGGTCAGGT GGAGTGGGAG
//