LOCUS Exported 2698 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2698 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 736..783 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2631..2647 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2648..2679 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..664 /label="Mfl359" /note="" /translation="MKWILKKNKMDSKFIIAICIIILYAIILLIVGILSYVYSVKHKPFKVINDEYLKEIAEYKKVEDEILRKLKPIKTKFELSENENIYFVDELEVSFNENENKKNKKKEFDDEYDQYIKNLKKNYSLFKIKGKQNELEKTLTYISNKRIVLDNGTEFKIIKFNNVILVENYVWNFNGEYLKTIYLKTKNEEIYFVTNDLKLKSIISKFRLENE" primer_bind complement(788..804) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(871..1459) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1650..2510) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2511..2615) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 ACAACGAGCA GACCGAATAG GGTCTCAAAT GAAATGGATC CTGAAGAAAA ATAAAATGGA 61 CAGCAAATTT ATTATTGCGA TTTGTATCAT CATTTTATAT GCGATCATTC TGCTGATCGT 121 TGGCATTCTG TCCTATGTTT ATAGCGTTAA GCACAAGCCG TTCAAGGTGA TCAACGACGA 181 GTACCTGAAA GAGATCGCGG AGTACAAAAA GGTGGAGGAT GAGATCCTGC GCAAGCTGAA 241 GCCGATCAAG ACCAAGTTTG AGCTGAGCGA GAATGAGAAC ATCTACTTCG TTGATGAATT 301 AGAGGTGTCC TTCAATGAAA ATGAGAATAA GAAAAATAAG AAAAAAGAGT TCGACGATGA 361 GTACGATCAA TACATTAAAA ACTTGAAGAA AAACTACTCC CTGTTCAAGA TCAAGGGTAA 421 GCAGAACGAA TTGGAAAAGA CCCTGACCTA TATCAGCAAC AAACGCATTG TTCTGGACAA 481 TGGTACTGAG TTCAAGATCA TCAAATTCAA TAACGTGATT CTGGTTGAGA ACTACGTCTG 541 GAATTTTAAT GGCGAGTATC TCAAGACGAT CTATTTAAAG ACGAAAAATG AAGAAATTTA 601 TTTTGTGACC AATGACCTGA AGCTCAAATC GATCATCTCG AAGTTCCGCC TGGAGAACGA 661 GTGAAGAGCT TAGAGACCGC TTGACGGAGC ATCTATCTCG CTGAGGTGTC AATCGTCGGA 721 GCCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT 781 TTGCATGGTC ATAGCTGTTT CCTGAGAGCT TGGCAGGTGA TGACACACAT TAACAAATTT 841 CGTGAGGAGT CTCCAGAAGA ATGCCATTAA TTTCCATAGG CTCCGCCCCC CTGACGAGCA 901 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 961 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 1021 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 1081 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 1141 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 1201 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 1261 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 1321 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 1381 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 1441 CAGAAAAAAA GGATCTCAAG AAGGCCTACT ATTAGCAACA ACGATCCTTT GATCTTTTCT 1501 ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA 1561 TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 1621 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC 1681 TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT 1741 ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGAACCACGC 1801 TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT 1861 GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 1921 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG 1981 TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT 2041 ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC 2101 AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT 2161 ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 2221 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC 2281 GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA 2341 CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC 2401 TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA 2461 AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 2521 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 2581 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACCT GCACCAGTCA GTAAAACGAC 2641 GGCCAGTAGT CAAAAGCCTC CGACCGGAGG CTTTTGACTT GGTCAGGTGG AGTGGGAG //