LOCUS       Exported     3436 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3436
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1474..1521
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3369..3385
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3386..3417
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1402
                     /label="hisS"
                     /note=""
                     /translation="MKTNKNKTNIKMIGFYHFFYFVKPYYLLINENVIIFKAKGDFMIQKPRGTQDLFLNNSKEWNAVEEKFRKVLNLFNYGEIITPMFESKELFVRGVGDSSDIVSKEMYEFTDRKGREYVLRPEGTAPTVRALIENKLYIQENLPYKTFYIGPIFRYERPQAGRYRQFNQLGVETFGIDSISHDVELISLGQSFLKELKINKDVIVEMNYLISGNERKEYELELKKYLNLFDDLCSDCEIRINKNVLRVLDCKIDGNKFAEAPKMTLFASQENKERLNKTFDQLKQLGIEAKINFNLVRGLDYYTGLVFEFKNIKTDQAIIAGGSYNNLVEELGGPNLPASGFAIGIERIMMILSDQEIKVADEDELDLFIIPLSDDAQFLTNKLLLEARTANLKVDTNWNIKNLKAGFKSAERLKSKNIVIIGENSINSDIYAIKDQKSGETKELKFKDIVKYLKGEK"
     primer_bind     complement(1526..1542)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1609..2197)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2388..3248)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3249..3353)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 ACAAGGAGTC GGCATATCAC GGTCTCAAAT GAAAACCAAT AAAAACAAAA CGAATATCAA
       61 GATGATTGGT TTCTACCATT TCTTTTACTT CGTGAAGCCT TATTATCTGC TGATCAATGA
      121 GAACGTCATT ATTTTCAAGG CGAAAGGCGA CTTCATGATT CAGAAGCCGC GCGGCACCCA
      181 GGACCTGTTC CTGAACAACA GCAAGGAGTG GAACGCTGTC GAGGAAAAAT TTCGCAAGGT
      241 TTTGAACCTG TTCAATTACG GCGAGATCAT TACTCCGATG TTTGAAAGCA AAGAGCTGTT
      301 TGTGCGCGGT GTCGGTGACA GCAGCGATAT TGTTAGCAAG GAGATGTACG AGTTTACGGA
      361 CAGAAAGGGT AGAGAGTATG TCCTGCGTCC GGAAGGTACC GCTCCAACCG TTCGTGCGCT
      421 GATTGAGAAC AAACTGTACA TCCAGGAGAA TCTGCCGTAC AAAACTTTCT ACATTGGCCC
      481 GATCTTCCGC TACGAACGTC CGCAAGCTGG TCGCTACCGT CAGTTCAACC AACTGGGCGT
      541 GGAAACCTTC GGTATTGATT CAATCAGCCA CGATGTAGAA CTGATCAGCC TGGGTCAGAG
      601 CTTTCTGAAA GAGCTGAAGA TTAATAAAGA CGTGATTGTT GAAATGAACT ATCTGATCAG
      661 CGGCAACGAA CGTAAAGAAT ACGAATTGGA GTTGAAAAAG TACTTGAATC TGTTCGACGA
      721 CCTGTGCAGC GATTGCGAGA TCCGCATTAA CAAGAATGTT CTGCGCGTTC TGGACTGTAA
      781 AATTGATGGC AATAAGTTCG CGGAGGCTCC GAAAATGACC CTGTTCGCTT CCCAGGAAAA
      841 CAAGGAGCGC CTGAATAAGA CCTTCGATCA ACTGAAGCAG CTGGGCATTG AAGCCAAAAT
      901 TAATTTCAAT CTGGTCCGCG GTCTTGACTA TTATACCGGC CTGGTGTTCG AGTTTAAAAA
      961 CATCAAAACC GACCAGGCGA TCATTGCCGG TGGTAGCTAC AACAACCTGG TGGAGGAACT
     1021 GGGCGGTCCA AATCTGCCGG CCAGCGGTTT CGCGATCGGT ATCGAGCGTA TTATGATGAT
     1081 TCTGAGCGAC CAGGAAATCA AAGTTGCAGA TGAGGACGAG CTGGACTTGT TTATCATTCC
     1141 GCTGTCCGAC GATGCGCAAT TCCTGACGAA TAAACTGCTG CTGGAAGCCC GCACCGCAAA
     1201 CTTGAAAGTG GATACTAACT GGAACATCAA AAACCTGAAA GCGGGCTTCA AGAGCGCCGA
     1261 ACGTCTGAAG TCCAAAAATA TCGTCATTAT TGGTGAGAAC AGCATCAACA GCGACATTTA
     1321 TGCGATTAAG GACCAAAAAA GCGGTGAAAC CAAAGAGCTG AAGTTCAAGG ATATCGTTAA
     1381 ATATCTGAAG GGTGAAAAGT GAAGAGCTTA GAGACCGCGC GGGTGATATT ACAACTCGCT
     1441 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1501 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1561 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1621 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1681 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1741 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1801 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1861 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1921 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1981 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     2041 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     2101 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     2161 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     2221 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     2281 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     2341 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     2401 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2461 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2521 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2581 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2641 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2701 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2761 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2821 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2881 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2941 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     3001 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     3061 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     3121 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     3181 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     3241 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     3301 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     3361 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3421 TCAGGTGGAG TGGGAG
//