LOCUS       Exported     3887 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3887
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3853
                     /translation="MLLIYQYWNSNSFEEEQEIVGKAKILKCKTNYIIVKINNTKFYIQANKNNYVVGMKLNIKGKVNQINSISNYYEFDFKEYLSKDNIRYQIKTSNLEILNNFNIRVLIYKHFNLNNAPLLINVLFLNKYDSSDLMVSELNQLGLKYLINFNFINMFLIFKLFNKITLRKFKYISTFILFVWSYLTCWPIMFTRIMFNQILDIFKTLKTDKYLKNLVSIVVIILIFPNFAFSSGFWYIVLIILFWSLVNKEKKLFNFIFFFMLLNLLNVYFTYQINGTSMFYAFLLSPIISVYYWITPLVYLINKDLLNNLYDVLLTVVILLKRISLILNVGYFNIIFILFGYLTIINFVCDLVSIKIKIYSIIAFFLFLVLNFLFKPSEYLTMLNVGNGNSFVYHNKWNNITIIFDAGVGKQRSPELVSNFLKYQGINKIDLIFISHTHEDHYNNLFSLKENFKIKQIILNEDILNTIKIKNIIINIFINPYANSENNKSLVLIVDIGNIRSLFMGDAEKETEMYLMNRIDFIYILNLKKVDILQIGHHGSKTSSSFEFINLIKPKIALISGENEGGNKKFPHKETIDTLKKLKIQYYITNGLDNIFVLFKSYKIIKK"
                     /label="Mfl369"
                     /note=""
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGACTGC ATACCAGGTT GGGTCTCAAA TGTTGCTGAT
     2041 CTACCAGTAT TGGAACTCAA ACAGCTTTGA AGAGGAGCAA GAGATCGTCG GCAAAGCAAA
     2101 AATCCTGAAA TGCAAAACCA ATTACATCAT CGTGAAAATC AATAATACCA AGTTTTATAT
     2161 TCAAGCGAAC AAAAACAATT ACGTCGTGGG CATGAAGCTG AACATTAAGG GCAAGGTGAA
     2221 CCAGATTAAC AGCATTAGCA ATTACTACGA ATTCGATTTC AAAGAGTACT TGAGCAAGGA
     2281 CAATATCCGT TACCAAATTA AAACCAGCAA TCTGGAGATC CTGAATAATT TCAACATCCG
     2341 TGTCCTGATC TACAAACACT TCAATTTGAA CAACGCCCCG CTGCTGATCA ATGTTCTGTT
     2401 CCTGAACAAG TATGACAGCA GCGATTTGAT GGTGAGCGAA CTGAATCAGC TGGGTCTGAA
     2461 ATACTTGATC AATTTCAACT TCATCAATAT GTTCTTGATC TTCAAGCTGT TCAATAAAAT
     2521 TACGTTGCGC AAATTCAAAT ATATCAGCAC GTTTATCCTG TTCGTGTGGA GCTACCTGAC
     2581 CTGTTGGCCG ATCATGTTCA CGCGCATCAT GTTTAATCAA ATTCTGGACA TTTTCAAAAC
     2641 CCTGAAAACG GACAAATATC TGAAAAACCT GGTTAGCATC GTTGTTATTA TTCTGATTTT
     2701 TCCGAACTTT GCATTCAGCT CTGGTTTCTG GTATATCGTC CTGATCATCT TATTTTGGTC
     2761 CCTGGTCAAC AAGGAGAAAA AACTGTTCAA TTTCATTTTC TTTTTCATGC TTCTGAATCT
     2821 GCTGAACGTG TACTTCACGT ACCAAATCAA TGGCACGAGC ATGTTCTACG CATTCCTGCT
     2881 TTCGCCTATC ATTAGCGTTT ACTATTGGAT CACCCCGCTG GTATATCTGA TTAATAAAGA
     2941 CTTGCTGAAT AACCTGTACG ACGTGCTGTT AACCGTTGTG ATCTTGCTGA AGCGCATTAG
     3001 CCTCATTTTG AATGTCGGCT ACTTTAATAT TATCTTTATC CTGTTCGGCT ACCTGACGAT
     3061 TATCAATTTT GTTTGCGATC TGGTTAGCAT TAAGATCAAA ATCTACAGCA TCATTGCCTT
     3121 TTTTCTGTTT CTGGTTTTGA ATTTTCTGTT CAAGCCGAGC GAGTACTTGA CGATGCTGAA
     3181 TGTTGGTAAT GGTAACAGCT TCGTTTATCA CAACAAGTGG AATAATATCA CCATCATTTT
     3241 CGACGCAGGC GTCGGTAAGC AGCGTAGCCC GGAACTGGTT AGCAATTTCC TGAAGTACCA
     3301 AGGCATTAAT AAAATTGATC TGATCTTCAT TAGCCACACC CACGAGGACC ATTACAACAA
     3361 CCTGTTTAGC TTGAAGGAAA ACTTTAAAAT TAAGCAAATC ATTCTGAACG AGGATATCCT
     3421 GAACACGATT AAAATTAAGA ATATTATTAT TAACATTTTC ATCAATCCAT ACGCCAATAG
     3481 CGAAAATAAT AAAAGCCTGG TTCTGATTGT GGATATTGGT AACATCCGTA GCCTGTTCAT
     3541 GGGTGACGCA GAGAAGGAAA CCGAAATGTA TCTGATGAAT CGTATCGACT TCATCTATAT
     3601 TCTCAATCTG AAGAAGGTCG ACATCCTGCA AATCGGTCAT CATGGCAGCA AGACCTCCAG
     3661 CAGCTTCGAG TTTATTAACC TGATTAAACC GAAAATCGCG CTGATTAGCG GTGAGAATGA
     3721 GGGCGGTAAT AAAAAGTTCC CGCACAAGGA AACGATTGAC ACGTTGAAGA AGCTGAAGAT
     3781 CCAGTACTAT ATTACCAACG GTCTGGACAA TATTTTTGTG CTGTTTAAGT CTTATAAAAT
     3841 TATTAAGAAG TGAAGAGCTT AGAGACCGTC CGTCGGAGTA ACAATCT
//