LOCUS Exported 2557 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2557 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 595..642 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2490..2506 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2507..2538 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..523 /label="Mfl370" /note="" /translation="MKKFQYLIAFTIILILVFVTSFFSIDNFSNKEEFIFQIRYELITNIENFTYPKKLPKNNYKNYWNEFQKVNSNLNWFEPIKINLKQDKIVLDIKDQYIQKINYLNIKMSDLLVFNLSSSYSIELFLIIDQAKPSSVIESEVKAFCESKNEQFLFEKGLSKLITF" primer_bind complement(647..663) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(730..1318) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1509..2369) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(2370..2474) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAGAAGTTC CAATATCTGA TCGCTTTCAC 61 CATTATCCTG ATTCTGGTTT TTGTCACTAG CTTCTTCAGC ATCGATAATT TTAGCAACAA 121 AGAGGAGTTC ATCTTTCAGA TCCGCTACGA GTTGATCACC AACATCGAAA ACTTCACGTA 181 CCCAAAGAAG CTGCCGAAGA ACAATTATAA AAATTACTGG AACGAGTTTC AGAAGGTTAA 241 TAGCAATCTG AACTGGTTTG AGCCTATTAA GATTAACCTT AAACAGGATA AAATCGTGTT 301 AGACATTAAG GACCAGTATA TTCAGAAAAT CAATTATCTC AATATTAAAA TGAGCGATCT 361 GCTGGTTTTT AACCTGAGCA GCAGCTACAG CATCGAGCTG TTTCTGATCA TCGACCAGGC 421 CAAGCCGAGC AGCGTTATTG AGTCCGAGGT GAAAGCGTTC TGCGAGTCCA AAAACGAGCA 481 ATTCCTGTTC GAGAAGGGTC TGTCCAAACT GATTACCTTT TGAAGAGCTT AGAGACCCGC 541 TGATGGTAAT TAGCCCTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 601 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 661 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 721 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 781 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 841 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 901 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 961 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 1021 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1081 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 1141 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1201 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 1261 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 1321 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 1381 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 1441 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 1501 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 1561 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 1621 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA 1681 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 1741 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 1801 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 1861 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 1921 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 1981 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 2041 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2101 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 2161 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 2221 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 2281 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 2341 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 2401 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 2461 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC 2521 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG //