LOCUS Exported 2602 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2602 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 640..687 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2535..2551 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2552..2583 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..568 /note="Could methylate the ribose at the nucleotide 34 wobble position in tRNA." /translation="MERQVNIVLYEPEIAQNVAAIIRTCVATNAKLHIIEPLGFIFKKEEMNRASAGTFDLVEYQLYDDWEDFCEKNPSIDLFCISRYGEKPHSDFDFNSYQKPVFVMFGKESTGIPKQIIKDNYENTFRLPMSPQTRSINVANTVGIVTYEILRQWDYPTLSKTEVQKGKDYIMSERWKDEK" /label="Mfl374" primer_bind complement(692..708) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(775..1363) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1554..2414) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2415..2519) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GGAGCGCCAA GTCAACATTG TGCTGTATGA 61 GCCGGAAATC GCGCAGAACG TCGCAGCGAT TATTAGAACG TGCGTGGCGA CCAATGCGAA 121 ACTGCACATC ATCGAACCGC TGGGCTTTAT TTTCAAGAAG GAGGAGATGA ATCGTGCAAG 181 CGCCGGCACC TTCGATCTGG TTGAGTATCA GCTGTATGAC GATTGGGAGG ATTTTTGCGA 241 GAAAAATCCA AGCATCGACC TGTTCTGCAT CTCCCGTTAT GGCGAAAAGC CGCACAGCGA 301 CTTTGATTTC AATAGCTATC AGAAGCCTGT GTTTGTCATG TTCGGTAAGG AGAGCACCGG 361 TATCCCGAAA CAAATTATCA AAGACAACTA CGAGAACACT TTCCGTTTAC CGATGAGCCC 421 GCAAACCCGC AGCATTAACG TGGCGAATAC CGTTGGTATC GTTACTTATG AGATCTTACG 481 CCAGTGGGAT TATCCGACCC TTAGCAAAAC CGAAGTTCAG AAGGGTAAAG ATTACATCAT 541 GTCCGAGCGC TGGAAAGACG AGAAGTGAAG AGCTTAGAGA CCCGCGCTAA TAGCTTTGCA 601 TTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC 661 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG 721 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA 781 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 841 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 901 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 961 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 1021 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 1081 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 1141 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 1201 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 1261 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 1321 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC 1381 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT 1441 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA 1501 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA 1561 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC 1621 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT 1681 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA 1741 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT 1801 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT 1861 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC 1921 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC 1981 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT 2041 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT 2101 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC 2161 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT 2221 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG 2281 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT 2341 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA 2401 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 2461 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 2521 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG 2581 ACTTGGTCAG GTGGAGTGGG AG //