LOCUS       Exported     2824 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2824
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      862..909
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2757..2773
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2774..2805
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..790
                     /label="Mfl376"
                     /note=""
                     /translation="MKAVSGLSNKIIVKEKVLVKESYSYVDKYLDRDNEIKIYEQLSNLKNDFMIIPFEWKINKTQMVSKTEFFDNAITLHETDICVNKMKSIIKIISKIHNLELIDIKVFNPKEFLNFFIKNTKECLFDLDFIKDQVNLIIDNYWSDNSKPVFSHNDLVKGNFINYKKGWKIIDFEYAMYNHYLFDYASFISESLNKKRWNVFLNLLNLSEAELNKITNLILYQNYLWIYWASYMFEQTNDQIFKDIAKEKFENIK"
     primer_bind     complement(914..930)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(997..1585)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1776..2636)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2637..2741)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACCGGAGC CATACAGTAC GGTCTCAAAT GAAAGCTGTG AGCGGTCTGA GCAACAAGAT
       61 CATTGTCAAA GAGAAGGTTC TGGTGAAAGA GAGCTACAGC TATGTGGATA AGTATTTGGA
      121 TCGCGATAAC GAGATTAAGA TTTACGAGCA GCTGTCAAAT CTGAAAAATG ACTTCATGAT
      181 TATCCCGTTT GAATGGAAGA TTAATAAGAC TCAAATGGTT AGCAAGACCG AGTTTTTTGA
      241 TAATGCCATC ACCCTGCATG AAACGGATAT TTGCGTTAAC AAGATGAAGT CTATCATCAA
      301 GATTATTAGC AAGATTCATA ATCTGGAGCT TATCGACATC AAAGTGTTTA ACCCGAAAGA
      361 ATTTCTGAAT TTCTTTATCA AGAATACCAA GGAATGCTTG TTTGACTTGG ACTTTATTAA
      421 GGATCAAGTG AATCTGATTA TTGATAACTA TTGGAGCGAT AACTCGAAGC CGGTCTTTAG
      481 CCATAATGAT CTGGTCAAAG GTAACTTCAT TAATTACAAG AAGGGTTGGA AAATCATCGA
      541 CTTTGAGTAC GCTATGTATA ATCACTACCT GTTCGACTAC GCTAGCTTTA TCAGCGAGAG
      601 CCTGAACAAG AAACGCTGGA ATGTGTTCCT GAATCTGCTG AATCTGAGCG AAGCCGAGTT
      661 GAACAAGATC ACCAATCTGA TCCTGTACCA AAACTACCTG TGGATTTATT GGGCGAGCTA
      721 TATGTTCGAG CAGACCAACG ACCAAATCTT TAAGGATATC GCCAAAGAAA AATTCGAAAA
      781 CATCAAGTGA AGAGCTTAGA GACCCGAGTG TAGTTCCCGT CAATCGCTGA GGTGTCAATC
      841 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
      901 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
      961 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     1021 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     1081 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     1141 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     1201 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     1261 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     1321 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     1381 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     1441 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     1501 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     1561 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     1621 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     1681 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     1741 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     1801 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     1861 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     1921 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     1981 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     2041 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     2101 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     2161 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     2221 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     2281 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     2341 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     2401 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     2461 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     2521 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     2581 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     2641 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     2701 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     2761 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     2821 GGAG
//