LOCUS Exported 3187 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3187 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1225..1272 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 3120..3136 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 3137..3168 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1153 /label="Mfl382" /note="" /translation="MTKEKEVKKPVAKKTETKPATKKAATPKSPAKKTATKPVAKNQPAKKVEEVKIEEVKVVETTEVTNVSSTKTTKSKTKKKKKEKLKLNFWKMTYMWLPMWNMMRKAKKISKKNLKSPGYFSEEYCYNFVKKAAKRILYVSDITVKCEGIENWLDRGVILAPNHQSNFDPVALVALNNFYLQQPLAFIAKKELWEDKKFGRFVRLMDCIPLDRKNPRSALEAFKEGKQLVVDFRRSLAIFPEGTRSHSQQVGEFQPAALKVAQMANAPVVPVTIINSYQVFSKDRPKHIEIKVVFGKPIMPLKHISLKTEDLTKNTRKVIIENMEKWVDKEILLDHKTINKINLKNREDFQKAGNSKPKKSKEKKKLKDIFKVVD" primer_bind complement(1277..1293) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1360..1948) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2139..2999) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(3000..3104) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACACGTGG CAAACATTCC GGTCTCAAAT GACCAAGGAG AAGGAAGTCA AAAAGCCGGT 61 CGCTAAAAAG ACCGAAACCA AGCCGGCAAC GAAAAAGGCC GCGACCCCGA AAAGCCCTGC 121 GAAGAAAACC GCCACGAAGC CGGTCGCGAA GAACCAACCG GCGAAGAAGG TTGAAGAAGT 181 CAAAATCGAA GAAGTGAAGG TGGTCGAAAC CACCGAAGTC ACGAATGTCT CTAGCACCAA 241 AACTACCAAG AGCAAAACCA AGAAGAAAAA GAAGGAGAAG TTGAAATTGA ACTTTTGGAA 301 AATGACGTAC ATGTGGCTGC CGATGTGGAA TATGATGCGT AAAGCGAAGA AAATTAGCAA 361 GAAGAACTTG AAAAGCCCGG GTTATTTTAG CGAGGAGTAC TGCTACAATT TCGTTAAAAA 421 AGCAGCCAAA CGTATCCTGT ATGTGAGCGA CATTACCGTG AAATGCGAGG GTATTGAGAA 481 CTGGCTGGAT CGCGGTGTTA TTCTGGCGCC GAACCACCAA TCTAATTTCG ACCCGGTCGC 541 GCTGGTTGCG TTGAATAATT TTTATCTGCA ACAGCCACTG GCCTTTATTG CGAAAAAAGA 601 ACTGTGGGAG GACAAGAAGT TCGGTCGTTT TGTGCGTCTG ATGGACTGCA TCCCGCTGGA 661 CCGCAAGAAT CCGCGCAGCG CGCTGGAGGC CTTCAAGGAA GGCAAACAAC TGGTGGTAGA 721 CTTCCGTCGT AGCTTAGCCA TCTTCCCAGA GGGTACCCGC AGCCATTCTC AACAGGTTGG 781 TGAGTTCCAG CCGGCTGCAC TGAAGGTTGC CCAAATGGCG AACGCTCCGG TCGTCCCGGT 841 CACCATTATC AACAGCTACC AGGTTTTTAG CAAGGACCGT CCAAAGCACA TTGAGATTAA 901 AGTGGTCTTT GGTAAGCCAA TTATGCCGCT GAAACATATC AGCCTCAAGA CCGAGGACCT 961 GACGAAAAAT ACCCGTAAAG TTATTATCGA GAACATGGAA AAGTGGGTGG ACAAGGAGAT 1021 CCTGCTGGAT CACAAAACGA TTAACAAGAT TAACTTGAAG AATCGTGAGG ACTTTCAAAA 1081 GGCGGGTAAC TCTAAGCCGA AGAAAAGCAA AGAGAAGAAA AAACTGAAGG ATATTTTCAA 1141 AGTCGTCGAT TGAAGAGCTT AGAGACCCCG AGATTTAGAC GGATGCTCGC TGAGGTGTCA 1201 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG 1261 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT 1321 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC 1381 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 1441 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 1501 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 1561 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 1621 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 1681 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 1741 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 1801 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 1861 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 1921 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG 1981 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC 2041 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 2101 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG 2161 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG 2221 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA 2281 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG 2341 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 2401 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC 2461 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA 2521 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG 2581 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT 2641 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 2701 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG 2761 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG 2821 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT 2881 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA 2941 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 3001 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 3061 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG 3121 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA 3181 GTGGGAG //