LOCUS       Exported     2536 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2536
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      574..621
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2469..2485
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2486..2517
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..502
                     /translation="MKRENFLNWEQFFMTVAKVCAMRSKDPSTQVGAILVNNLNQIISTGYNGFPRGVNDDEFPWTREGEWIDTKYPYVAHAELNAIVSARTNLTDSDVYVTLFPCNECTKIIIQAGIKKVYYLEDKYKDSNEVKASKRMLDAAKIEYKQINDFNVKISID"
                     /label="Mfl385"
                     /note=""
     primer_bind     complement(626..642)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(709..1297)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1488..2348)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2349..2453)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAAACGTGAG AACTTTCTGA ACTGGGAACA
       61 ATTTTTCATG ACGGTCGCCA AAGTGTGTGC AATGCGTTCG AAGGACCCGT CTACGCAAGT
      121 TGGCGCCATC TTGGTGAACA ACCTGAACCA AATCATTTCG ACTGGCTATA ACGGCTTCCC
      181 GCGCGGTGTT AATGACGACG AATTTCCATG GACCCGTGAG GGCGAGTGGA TTGACACCAA
      241 ATATCCGTAC GTGGCGCACG CGGAGCTGAA TGCAATTGTG TCCGCACGCA CCAACCTGAC
      301 CGACAGCGAC GTGTACGTCA CCCTGTTTCC GTGTAATGAG TGTACCAAGA TCATTATCCA
      361 GGCAGGCATC AAGAAAGTTT ACTATCTGGA GGATAAATAC AAGGACTCGA ACGAAGTGAA
      421 AGCTAGCAAA CGTATGTTAG ATGCGGCCAA AATCGAGTAT AAGCAAATTA ACGACTTCAA
      481 TGTTAAGATC TCAATCGATT GAAGAGCTTA GAGACCGGAC CTTGACGCCT AATACTCGCT
      541 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
      601 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
      661 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
      721 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
      781 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
      841 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
      901 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
      961 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1021 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1081 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1141 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1201 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1261 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1321 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1381 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1441 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1501 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     1561 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     1621 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     1681 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     1741 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     1801 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     1861 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     1921 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     1981 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2041 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2101 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2161 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2221 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2281 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2341 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2401 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2461 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     2521 TCAGGTGGAG TGGGAG
//