LOCUS       Exported     2686 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2686
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      724..771
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2619..2635
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2636..2667
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..652
                     /label="Mfl388"
                     /note=""
                     /translation="MFKNQISNLIFGLKSYDFKWKFKLIIATPIMSFLILLDWIIKWVVVATMKENDSKTFINGFLNIQYKINLGSAYGRGDYADGLAKTVTLAALFVALLIIVFIFLNDKKWIITCSILLAGGFANLLARAWAPATIRDGQEIYGGVVDMFVWGFDFLGSSGYIFNLADMWVNIGIGIGAVCFIIEMINIFKPKKQKEINKEVDKNENKS"
     primer_bind     complement(776..792)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(859..1447)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1638..2498)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2499..2603)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGAATTACT GACCCCTCGG GGTCTCAAAT GTTCAAAAAT CAGATCAGCA ATCTGATCTT
       61 CGGCCTCAAG AGCTACGACT TCAAGTGGAA ATTCAAACTG ATTATCGCAA CCCCAATCAT
      121 GTCGTTCCTG ATTCTGCTGG ACTGGATCAT TAAGTGGGTT GTCGTGGCGA CCATGAAGGA
      181 GAACGATAGC AAGACGTTCA TCAATGGTTT CCTGAACATC CAGTACAAAA TCAATCTGGG
      241 CAGCGCTTAT GGCCGTGGTG ACTATGCCGA CGGTCTGGCG AAAACGGTGA CCCTGGCTGC
      301 GCTGTTCGTG GCCTTGCTGA TTATCGTGTT CATTTTCCTG AATGATAAGA AATGGATCAT
      361 TACGTGCAGC ATTCTCCTGG CCGGTGGTTT TGCGAATCTG CTGGCACGTG CATGGGCACC
      421 GGCGACCATC CGCGACGGCC AGGAGATTTA TGGCGGCGTT GTCGATATGT TTGTTTGGGG
      481 TTTCGACTTC CTGGGTTCTA GCGGCTATAT TTTCAATCTG GCTGACATGT GGGTGAATAT
      541 CGGTATTGGT ATTGGTGCGG TTTGTTTCAT TATCGAGATG ATCAACATCT TTAAACCGAA
      601 AAAGCAAAAA GAGATCAATA AGGAAGTTGA CAAAAACGAG AACAAATCCT GAAGAGCTTA
      661 GAGACCGCTC GATGCCATAG TTCCTTCGCT GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA
      721 TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCATGGTCAT
      781 AGCTGTTTCC TGAGAGCTTG GCAGGTGATG ACACACATTA ACAAATTTCG TGAGGAGTCT
      841 CCAGAAGAAT GCCATTAATT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG
      901 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC
      961 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC
     1021 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC
     1081 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG
     1141 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC
     1201 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA
     1261 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC
     1321 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC
     1381 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG
     1441 ATCTCAAGAA GGCCTACTAT TAGCAACAAC GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     1501 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC
     1561 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG
     1621 TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT
     1681 CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG
     1741 GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AACCACGCTC ACCGGCTCCA
     1801 GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT
     1861 TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA
     1921 GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG
     1981 TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC
     2041 ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG
     2101 GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA
     2161 TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT
     2221 ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC
     2281 AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC
     2341 TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA
     2401 TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA
     2461 AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT
     2521 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA
     2581 AATAAACAAA TAGGGGTTCC GCGCACCTGC ACCAGTCAGT AAAACGACGG CCAGTAGTCA
     2641 AAAGCCTCCG ACCGGAGGCT TTTGACTTGG TCAGGTGGAG TGGGAG
//