LOCUS Exported 3037 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3037 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1075..1122 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2970..2986 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2987..3018 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..1003 /note="" /translation="MSEYSTLAHKMIQMWNSSFSKTIKQREEAASLKINFVKTINNFNSTNRNSNKKLKIKEYKSPTANFTTLSKDGVKIVASVWLNPKPSNKWVIGVHGFNSGRFNVLYLTWHYRSLGYNIITFDFRNHGSSDSDVVTWGYKEKWDLMTIITWVTQNYKPEEIGLVGTSMGGFTVNYLALTEEKFVKENNIKWAISDSAYMSVSKLLRSMVANNAPKIFENYVNLVLEDMLKIYKNEYGVNLVELDFINLIETKHKYIPIMYIHNRFDRVTNFLDSFKMQDVKNNIEESKVNELIIYDTGINHTKSIIKFTEDYILRTTDFVKKHQI" /label="Mfl390" primer_bind complement(1127..1143) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1210..1798) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1989..2849) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2850..2954) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACACGTGG CAAACATTCC GGTCTCAAAT GTCGGAGTAC AGCACCCTGG CGCACAAGAT 61 GATCCAGATG TGGAATAGCT CCTTCAGCAA GACCATCAAA CAGCGTGAAG AAGCAGCGAG 121 CCTGAAGATC AATTTTGTCA AAACCATTAA TAATTTCAAC TCAACCAACC GTAATAGCAA 181 CAAGAAACTG AAGATCAAGG AATACAAGTC CCCGACGGCT AACTTTACCA CTCTGTCCAA 241 AGACGGTGTG AAAATTGTTG CGTCCGTTTG GTTAAACCCG AAGCCATCGA ACAAATGGGT 301 TATTGGTGTG CATGGCTTTA ACAGCGGTCG TTTCAACGTT CTGTATCTGA CGTGGCACTA 361 TCGCAGCCTG GGCTATAACA TCATTACGTT CGACTTCCGT AATCATGGTA GCAGCGACTC 421 CGATGTGGTG ACCTGGGGCT ATAAAGAAAA ATGGGATTTA ATGACTATTA TCACGTGGGT 481 TACGCAAAAC TATAAGCCGG AGGAGATTGG CCTGGTGGGC ACCAGCATGG GTGGTTTTAC 541 CGTGAACTAT CTGGCGCTGA CGGAAGAGAA GTTTGTGAAG GAAAATAACA TTAAGTGGGC 601 GATTAGCGAC AGCGCGTACA TGAGCGTGAG CAAGCTGCTG CGTAGCATGG TGGCCAACAA 661 CGCGCCGAAG ATTTTTGAGA ATTACGTCAA CCTGGTCTTA GAGGACATGC TGAAAATCTA 721 CAAAAACGAG TACGGTGTCA ACTTGGTGGA ACTGGACTTT ATTAATCTGA TCGAAACGAA 781 GCACAAGTAT ATCCCGATTA TGTACATTCA CAATCGTTTC GATCGTGTCA CGAATTTCCT 841 GGACTCCTTC AAAATGCAGG ACGTTAAAAA CAATATCGAA GAGTCCAAAG TGAATGAGTT 901 GATTATCTAC GATACCGGTA TTAATCACAC CAAAAGCATT ATTAAATTCA CGGAAGATTA 961 TATCCTGCGC ACTACGGATT TTGTTAAGAA ACACCAGATT TGAAGAGCTT AGAGACCGCC 1021 ATTGGAAAAC TGAACGTCGC TGAGGTGTCA ATCGTCGGAG CCGCTGAGCA ATAACTAGCA 1081 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCATGGTCA TAGCTGTTTC 1141 CTGAGAGCTT GGCAGGTGAT GACACACATT AACAAATTTC GTGAGGAGTC TCCAGAAGAA 1201 TGCCATTAAT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 1261 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 1321 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 1381 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 1441 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 1501 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 1561 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 1621 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 1681 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 1741 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 1801 AGGCCTACTA TTAGCAACAA CGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG 1861 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG 1921 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG 1981 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT 2041 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 2101 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GAACCACGCT CACCGGCTCC AGATTTATCA 2161 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC 2221 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT 2281 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG 2341 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 2401 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG 2461 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA 2521 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA 2581 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA 2641 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 2701 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT 2761 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA 2821 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT 2881 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 2941 ATAGGGGTTC CGCGCACCTG CACCAGTCAG TAAAACGACG GCCAGTAGTC AAAAGCCTCC 3001 GACCGGAGGC TTTTGACTTG GTCAGGTGGA GTGGGAG //