LOCUS       Exported     3253 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3253
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1291..1338
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     3186..3202
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      3203..3234
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1219
                     /label="Mfl392"
                     /note="Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity."
                     /translation="MNNNTQEFSQKAKIKVIGVGGGGNNAVSRMFEQGAHGVDFYIANTDAQVLAGSNVPNKIILGEKSTKGLGAGANPEVGKTAALESENDLRAALEGADLIFVTAGMGGGTGTGAAPVIARIAQETGALVVAIVTKPFRFEGKYRNTFAEEGIIELKKYVDSTIVISNDRLLEFIGAKPIQEAFAEADAILKQGVQTITDLIAVPALINLDFADVKTVMSKKGNALFGIGLGTGPDKANLAANDAISSTLLEAAIVGAKDVIVNVTGGEGISLNDAYDVVDVVNQAIDNPEVNIVFGVAINKELTEKDELVVTVIATGFDEEMIKSSANLGTKSNAASTFANLRTSSLYKSTHVEEEQKAPTSFEEDVLHAHQTMHSVNSGASTYSDDTEDDFPTFLK"
     primer_bind     complement(1343..1359)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1426..2014)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2205..3065)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3066..3170)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGCGTCAT TTTACACGGC GGTCTCAAAT GAATAATAAT ACCCAGGAAT TTAGCCAAAA
       61 AGCAAAAATT AAAGTTATTG GTGTGGGTGG TGGCGGTAAT AACGCAGTCT CTCGCATGTT
      121 CGAGCAGGGT GCGCACGGCG TCGATTTTTA TATCGCGAAC ACCGACGCAC AGGTGTTGGC
      181 GGGCAGCAAC GTGCCGAACA AAATCATCCT GGGTGAAAAG AGCACCAAAG GCTTGGGCGC
      241 GGGTGCGAAT CCGGAGGTGG GCAAAACCGC CGCGCTGGAA AGCGAAAACG ATTTGCGTGC
      301 GGCACTGGAG GGCGCAGATC TGATTTTCGT TACCGCGGGT ATGGGTGGTG GTACGGGCAC
      361 CGGTGCTGCA CCGGTGATTG CACGTATTGC TCAAGAAACC GGCGCACTGG TCGTTGCGAT
      421 TGTCACCAAA CCGTTCCGCT TTGAAGGCAA ATACCGTAAT ACGTTTGCAG AAGAAGGCAT
      481 CATTGAACTC AAAAAATATG TTGATAGCAC GATTGTTATC TCTAACGATC GTCTGCTGGA
      541 GTTCATTGGT GCGAAGCCGA TCCAGGAGGC GTTTGCCGAG GCGGATGCGA TCCTGAAACA
      601 AGGTGTCCAG ACTATTACGG ACCTGATCGC GGTGCCGGCC TTGATTAACC TGGATTTCGC
      661 GGATGTGAAA ACGGTTATGA GCAAAAAGGG TAATGCATTG TTCGGTATTG GTCTGGGTAC
      721 CGGTCCGGAC AAAGCGAACC TGGCCGCAAA TGACGCGATC TCATCTACTC TGCTGGAGGC
      781 TGCCATTGTC GGTGCAAAAG ACGTGATTGT GAACGTGACG GGCGGTGAGG GCATTTCGCT
      841 GAACGATGCA TATGACGTTG TCGACGTGGT CAATCAGGCG ATTGACAATC CGGAGGTTAA
      901 CATTGTTTTC GGTGTTGCTA TTAACAAAGA ATTGACCGAG AAAGATGAAC TGGTCGTGAC
      961 GGTTATCGCG ACCGGTTTCG ACGAGGAGAT GATCAAAAGC AGCGCTAATC TGGGTACGAA
     1021 AAGCAACGCT GCAAGCACGT TCGCGAATCT GCGCACCTCC TCGTTGTACA AGAGCACCCA
     1081 TGTAGAGGAG GAGCAGAAGG CTCCGACGAG CTTTGAGGAG GACGTCCTGC ACGCGCATCA
     1141 AACGATGCAC AGCGTCAACA GCGGTGCGAG CACCTACTCG GACGACACGG AAGATGATTT
     1201 TCCTACCTTT CTGAAGTGAA GAGCTTAGAG ACCCGCTCAC GGAGTGTACT AATCGCTGAG
     1261 GTGTCAATCG TCGGAGCCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG
     1321 GTCTTGAGGG GTTTTTTGCA TGGTCATAGC TGTTTCCTGA GAGCTTGGCA GGTGATGACA
     1381 CACATTAACA AATTTCGTGA GGAGTCTCCA GAAGAATGCC ATTAATTTCC ATAGGCTCCG
     1441 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     1501 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     1561 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     1621 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     1681 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     1741 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     1801 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     1861 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     1921 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     1981 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGGC CTACTATTAG CAACAACGAT
     2041 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT
     2101 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT
     2161 TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC
     2221 AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC
     2281 GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA
     2341 CCGCGAGAAC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG
     2401 GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC
     2461 CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT
     2521 ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA
     2581 CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT
     2641 CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA
     2701 CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC
     2761 TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA
     2821 ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT
     2881 TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC
     2941 ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA
     3001 AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA
     3061 CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC
     3121 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACCTGCACC
     3181 AGTCAGTAAA ACGACGGCCA GTAGTCAAAA GCCTCCGACC GGAGGCTTTT GACTTGGTCA
     3241 GGTGGAGTGG GAG
//