LOCUS       Exported     3370 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3370
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1408..1455
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3303..3319
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3320..3351
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1336
                     /label="Mfl393"
                     /note=""
                     /translation="MLKNNKTFTTVEIRKSVVKINAYRVLKNNIISIFENEIEAKNNSTFLNENGVVRDSISTLLKKDISAMIKSIVKEQSELKDNKIFMVVPSSTYSFTTKKKTISSEMPILMTEEYVNDCFKKIVKREQQANNAYHLDIELVQVKIDGNKQEVNKLNVQGKEIELIISIKSIYKKVFESHKNVIEKIEEGKDSYMTNLEALYNSIQRPSKDESDIIVVDWKEDHIEAGLFKKGAFVEYASTNNGMNSIIKKISNEFGLSDEMSKHYLYNNINFDSKNILKTVLLKFKNSLVSKTLSGEQIQLIVKKAVKNAYKEIKESFVAKEKIDFYVTPIFNFGLIQEIPNGVSLISEAKVSSDYVNQVKVIGALKNNEHFESYGLISKITSKFIFDEFKAKKQKIKNDVINMQFAFFDDFGSQNQASGQLYLKNDGILIDKVEA"
     primer_bind     complement(1460..1476)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1543..2131)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2322..3182)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3183..3287)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GCTGAAAAAT AATAAGACGT TCACGACCGT
       61 GGAAATCAGA AAGAGCGTTG TCAAAATCAA TGCTTACCGC GTCCTGAAAA ACAACATTAT
      121 CAGCATCTTC GAAAATGAGA TCGAAGCAAA AAACAATTCG ACCTTCCTGA ACGAAAACGG
      181 TGTCGTCCGT GATTCCATCA GCACCCTGTT GAAAAAGGAC ATTTCCGCTA TGATTAAGTC
      241 TATTGTCAAG GAGCAATCCG AATTGAAGGA CAACAAAATT TTCATGGTTG TCCCGAGCAG
      301 CACCTACAGC TTCACCACCA AGAAAAAGAC CATCAGCTCC GAAATGCCGA TTTTAATGAC
      361 CGAAGAATAT GTTAATGACT GTTTCAAGAA GATCGTGAAA CGTGAGCAAC AAGCTAACAA
      421 TGCATACCAC CTGGATATCG AACTGGTGCA AGTCAAAATT GATGGTAATA AGCAAGAAGT
      481 CAATAAACTG AATGTCCAGG GTAAAGAAAT CGAACTGATC ATTAGCATTA AGTCTATTTA
      541 CAAAAAAGTT TTCGAATCAC ATAAAAATGT GATCGAAAAA ATTGAGGAAG GTAAAGATAG
      601 CTACATGACG AACCTGGAGG CACTGTACAA CAGCATCCAG CGTCCGTCGA AGGATGAGAG
      661 CGATATCATT GTAGTGGACT GGAAAGAGGA CCACATCGAG GCGGGCCTGT TTAAAAAAGG
      721 TGCGTTTGTT GAGTACGCTA GCACTAATAA CGGTATGAAT TCGATTATCA AGAAAATCAG
      781 CAACGAGTTC GGTCTGTCTG ACGAGATGAG CAAGCACTAC TTGTACAATA ATATCAACTT
      841 TGATAGCAAG AATATTTTAA AGACCGTCTT ACTGAAGTTT AAGAATAGCC TGGTCAGCAA
      901 AACGCTGAGC GGTGAGCAAA TCCAGCTGAT CGTGAAAAAG GCGGTCAAAA ACGCATATAA
      961 GGAGATCAAA GAAAGCTTTG TGGCGAAGGA GAAAATCGAT TTTTATGTTA CTCCGATCTT
     1021 TAACTTTGGT TTGATCCAAG AGATCCCGAA CGGCGTTTCG CTGATTAGCG AAGCTAAGGT
     1081 GTCGAGCGAT TACGTGAACC AGGTGAAAGT CATCGGTGCA CTGAAAAATA ACGAGCACTT
     1141 CGAAAGCTAC GGTCTGATCA GCAAGATTAC CAGCAAGTTC ATTTTTGACG AGTTTAAAGC
     1201 TAAAAAGCAG AAGATTAAGA ATGATGTCAT TAACATGCAA TTCGCCTTCT TCGACGACTT
     1261 TGGCAGCCAG AATCAGGCCT CTGGCCAACT GTATCTGAAA AATGACGGTA TCCTGATTGA
     1321 TAAGGTGGAA GCCTGAAGAG CTTAGAGACC GCGGTCGCTA TACATACCTT CGCTGAGGTG
     1381 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1441 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1501 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1561 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1621 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1681 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1741 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1801 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1861 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1921 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1981 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     2041 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     2101 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     2161 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     2221 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     2281 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     2341 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2401 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2461 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2521 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2581 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2641 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2701 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2761 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2821 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2881 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2941 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     3001 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     3061 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     3121 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     3181 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     3241 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     3301 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     3361 GGAGTGGGAG
//