LOCUS       Exported     4427 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4427
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4393
                     /translation="MSKKIKLPIFQIRGSFIVPGIKENLEVGRKNTLASVNYAIKNSNNQMIAIPQIDASVEKPEFSDLHEFGILIDFEVIKEWKDNSLTISTNPIQRCKVISFFENEDQVPYAEVELIESINDFSDEELKELIEKISDAIKTKASLVTKQIKQLISGESDDLSLAFDSIMFKLAPSKILTNPEYITSPSLKTRWSIIEKIIFAEDGIITRNAESIDAARQKNEIEQELNHKLKEKMDKQQKEYYLREKMRIIKDELEDEDDSDDSSLEKYKERLAKEPFPEEVKRKIMASIKRVEALQSGTPEWNTEKNYIDWMMSIPWWEETEDLTDLKYAKKILDKHHYGMKKVKERIIEYLAVKTKTKSLKAPIITLVGPPGVGKTSLAKSIAEAVGKNFVKVSLGGVKDESEIRGHRKTYVGSMPGRIIQTMKRAKVKNPLFLLDEIDKMASDHRGDPASAMLEVLDPEQNKEFSDHYIEEPYDLSQVMFIATANYPEDIPEALYDRMEIINLSSYTEIEKVKIAQDYLVPKAIEQHELTSEEISFTEGAINEIIKYYTREAGVRQLERHINSIIRKYIVKNLNGEMDKIVIDEKQVNDLLGKRIFDHTEKQEESQIGVVTGLAYTQFGGDILPIEVSLYPGKGNLILTGKLGEVMKESATIALTYVKSNFEKFGVDKKVFEENDIHVHVPEGAVPKDGPSAGITITTALISALSDKPVSKEIGMTGEITLRGNVLPIGGLREKSISASRSGLKTIIIPKKNERDLDEIPDEVKAKLKIIPAEKYEEVFAIVFKTK"
                     /label="Mfl404"
                     /note="ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner."
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GACAACGAGC AGACCGAATA GGGTCTCAAA TGAGCAAGAA
     2041 AATTAAGCTT CCGATTTTCC AGATTCGCGG CAGCTTTATT GTGCCAGGCA TCAAAGAGAA
     2101 TCTGGAAGTC GGCCGTAAGA ATACCCTGGC AAGCGTTAAT TACGCCATCA AAAACAGCAA
     2161 CAACCAAATG ATTGCAATCC CACAAATTGA CGCAAGCGTC GAAAAACCAG AGTTTAGCGA
     2221 TCTGCATGAG TTCGGTATCC TGATCGATTT CGAAGTGATT AAGGAATGGA AGGATAACAG
     2281 CTTAACCATT AGCACCAACC CGATTCAACG TTGCAAGGTC ATTAGCTTTT TTGAGAATGA
     2341 GGACCAGGTT CCGTATGCGG AAGTTGAGCT GATTGAGTCG ATTAACGACT TCTCCGATGA
     2401 GGAGCTGAAA GAGCTGATTG AGAAAATTTC CGATGCGATC AAGACCAAGG CGAGCCTGGT
     2461 AACCAAACAG ATTAAACAGC TGATTTCAGG TGAATCTGAT GATTTGTCAT TGGCGTTTGA
     2521 CTCCATCATG TTTAAGCTGG CGCCGAGCAA AATCCTGACC AACCCGGAGT ATATTACCAG
     2581 CCCGAGCCTG AAAACCCGTT GGAGCATTAT CGAGAAAATC ATCTTTGCGG AGGACGGTAT
     2641 CATTACCCGC AATGCAGAGA GCATTGACGC GGCGCGTCAA AAGAATGAAA TCGAACAGGA
     2701 ACTGAACCAC AAACTGAAAG AGAAAATGGA CAAACAGCAA AAGGAGTATT ACTTGCGTGA
     2761 GAAAATGCGC ATTATTAAAG ACGAATTGGA GGACGAGGAC GATAGCGACG ACTCCTCTCT
     2821 GGAGAAATAC AAAGAGCGCC TGGCCAAAGA ACCTTTCCCG GAGGAGGTCA AACGTAAGAT
     2881 TATGGCGAGC ATTAAACGTG TGGAAGCACT GCAGTCGGGT ACGCCGGAAT GGAATACCGA
     2941 GAAAAACTAC ATCGACTGGA TGATGTCTAT TCCGTGGTGG GAAGAAACCG AGGACTTGAC
     3001 CGACTTAAAA TACGCGAAGA AAATCTTGGA TAAACACCAT TACGGTATGA AGAAAGTGAA
     3061 AGAACGCATT ATCGAGTACC TGGCTGTCAA GACCAAGACC AAAAGCTTGA AAGCGCCGAT
     3121 CATCACGCTT GTCGGTCCGC CGGGTGTAGG CAAGACCTCT CTCGCAAAGA GCATTGCAGA
     3181 GGCAGTCGGT AAGAACTTCG TGAAGGTATC CCTGGGTGGT GTTAAGGATG AAAGCGAGAT
     3241 TCGCGGTCAC CGTAAAACTT ACGTGGGTAG CATGCCGGGT CGCATTATCC AGACGATGAA
     3301 ACGTGCGAAA GTAAAAAACC CACTGTTTCT GCTGGACGAG ATTGACAAGA TGGCCTCCGA
     3361 TCATCGTGGT GATCCGGCGA GCGCAATGCT GGAAGTCCTG GATCCAGAAC AAAACAAGGA
     3421 GTTCAGCGAT CACTATATCG AGGAGCCGTA CGACCTGTCA CAAGTTATGT TTATTGCGAC
     3481 CGCAAACTAT CCGGAAGATA TCCCGGAAGC TCTGTATGAC CGTATGGAAA TTATCAATCT
     3541 GAGCTCGTAC ACCGAAATTG AAAAGGTGAA GATTGCGCAA GACTACCTGG TGCCGAAGGC
     3601 GATTGAACAG CATGAACTGA CCAGCGAAGA AATTTCTTTC ACTGAAGGTG CTATCAACGA
     3661 AATTATTAAG TACTACACCC GCGAAGCGGG TGTTCGCCAA CTGGAGCGTC ATATTAATAG
     3721 CATCATCCGT AAGTATATCG TGAAGAATCT GAATGGTGAG ATGGACAAAA TCGTTATTGA
     3781 TGAAAAACAA GTTAACGATC TGCTGGGCAA ACGTATCTTT GACCACACCG AAAAGCAGGA
     3841 GGAATCTCAA ATCGGTGTGG TTACCGGTCT GGCTTATACC CAGTTCGGTG GTGACATTCT
     3901 GCCGATCGAA GTCAGCCTGT ACCCGGGTAA GGGCAATCTG ATTCTGACCG GCAAGCTGGG
     3961 TGAAGTTATG AAAGAGTCTG CTACGATTGC ACTGACGTAC GTCAAGAGCA ACTTTGAGAA
     4021 ATTCGGCGTT GACAAAAAGG TGTTCGAAGA GAACGATATT CACGTTCACG TTCCAGAAGG
     4081 TGCCGTCCCG AAGGACGGCC CGAGCGCGGG TATCACCATC ACCACCGCGC TCATCTCTGC
     4141 TCTGTCTGAC AAACCTGTCT CTAAAGAGAT CGGCATGACG GGTGAGATCA CCTTGCGCGG
     4201 TAATGTTCTG CCGATCGGTG GTCTGCGTGA GAAATCTATT TCCGCCTCCC GTAGCGGCCT
     4261 CAAGACCATT ATTATTCCGA AGAAGAACGA ACGTGACCTG GATGAAATCC CGGATGAAGT
     4321 GAAAGCGAAG CTGAAGATTA TTCCGGCGGA AAAATACGAG GAGGTGTTCG CCATCGTGTT
     4381 CAAGACCAAG TGAAGAGCTT AGAGACCCGC TAAGATCCGT AGGTCTG
//