LOCUS       Exported     4193 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4193
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4159
                     /label="Mfl410"
                     /note=""
                     /translation="MFKLKAKVNKETFKLSMKECVKKAWIWDNKENFASAVNWKKDRILEIDADANFDDENEFSDSAESIDIFSAYLNIESLNENEKEEFLKKWNSAWDSETGFDPELFAGETIEDGNAFGEKVREYFDMKNIIANDKRSEKKSTANLDELKFFEAEKKTKELLEENKCEYIYEAAFSFDDEKIRTRCDVLKIKDNKHVEIIEAKATTKVKAEHFFDLMYQVFVLEKCGFIVDDICIAKINSDYVMNSDLIITEKTFGEQAKEFINKLGKIKYEEIKDFVKSDFTVEYDNEPIEVDLNKLIYLDYLTYGKNAKRPTLKDDLKAFREKFDLDQIFMNLSNHLNMKYENGEIPEFLHNKKCTLNYNKDRAGNWDHGFNHDEYDNCFHVMNWFDKDEPGFWNIGKFKRSFKSHVIRNSKSPYFKDYESLFEPSIVLNKKGESFFETNRIAKRMFEVYNLKKQYPEDESKWRVIDLDNLEWINNLLSVYENYPIYMYDFETSKWAIPRFNLVNTYYQTPFQYSIDVIVDNNYDYNKPETMKHYSFLSNEQDTDPRIKFIENFIKDSFKHGPGVYVAYNKSFEQGVLRKLAMMFPKYAKPLAYIIQNTVDLMDFFTSDTKVTGRPAFLIYHPYFKGSYSIKKTQPSLDPSFSYNDLVINKGDKASETFRKFVDGRIPKAAWDLKVKEGMLKYCDRDTLAMVVILKKVKELVEAHNGKN"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCAAA TGTTCAAACT
     2041 GAAGGCCAAA GTGAACAAAG AAACGTTTAA ATTGAGCATG AAGGAGTGCG TGAAAAAGGC
     2101 GTGGATCTGG GACAACAAAG AGAATTTCGC AAGCGCTGTA AACTGGAAAA AGGATCGTAT
     2161 CCTGGAGATC GATGCGGACG CGAATTTCGA TGACGAAAAC GAATTCAGCG ACAGCGCGGA
     2221 AAGCATCGAT ATCTTTAGCG CATACTTGAA CATTGAAAGC CTGAATGAAA ATGAGAAAGA
     2281 GGAGTTCCTG AAAAAATGGA ACAGCGCATG GGATTCCGAA ACCGGCTTCG ATCCGGAGCT
     2341 GTTCGCTGGC GAAACGATTG AAGATGGCAA TGCGTTTGGT GAAAAAGTGC GTGAATACTT
     2401 TGACATGAAG AACATCATTG CAAACGACAA ACGCTCCGAG AAAAAGAGCA CCGCGAACCT
     2461 GGACGAACTG AAGTTTTTTG AGGCAGAGAA AAAGACCAAA GAACTGCTGG AAGAGAACAA
     2521 GTGTGAATAC ATTTATGAGG CGGCCTTTTC TTTCGATGAT GAGAAAATCC GTACGCGTTG
     2581 CGACGTTCTG AAGATCAAAG ATAATAAACA CGTTGAGATC ATCGAAGCCA AAGCGACGAC
     2641 GAAGGTTAAA GCTGAACATT TTTTCGACTT AATGTACCAA GTGTTCGTGT TGGAGAAGTG
     2701 TGGTTTTATT GTGGATGATA TCTGTATTGC CAAGATCAAT AGCGACTATG TGATGAACAG
     2761 CGACTTAATT ATCACCGAGA AAACGTTCGG TGAACAGGCC AAGGAGTTTA TCAACAAGCT
     2821 GGGTAAGATT AAGTACGAGG AAATCAAGGA CTTTGTTAAG AGCGACTTCA CTGTTGAGTA
     2881 CGACAATGAG CCGATCGAGG TGGATCTGAA TAAACTGATC TACTTGGATT ATTTAACGTA
     2941 CGGTAAAAAC GCGAAGCGTC CGACCCTGAA GGATGATCTG AAGGCGTTCC GCGAAAAATT
     3001 TGATTTGGAT CAGATCTTCA TGAACCTCAG CAACCATCTG AACATGAAGT ATGAGAACGG
     3061 TGAAATCCCG GAGTTCCTGC ACAACAAGAA ATGTACCTTG AATTACAATA AAGATAGAGC
     3121 GGGCAATTGG GACCACGGTT TCAACCATGA CGAGTATGAC AACTGCTTTC ATGTTATGAA
     3181 TTGGTTCGAT AAGGATGAAC CTGGTTTCTG GAATATTGGT AAGTTCAAGC GCAGCTTCAA
     3241 AAGCCACGTC ATCCGCAACT CGAAATCCCC GTATTTCAAG GACTACGAAA GCCTGTTTGA
     3301 GCCAAGCATT GTGTTGAATA AAAAAGGCGA GTCGTTCTTC GAAACGAATC GTATCGCGAA
     3361 ACGTATGTTC GAAGTGTACA ATCTGAAAAA ACAGTACCCG GAGGACGAAT CCAAATGGCG
     3421 TGTTATTGAC TTGGACAATC TGGAGTGGAT CAATAATTTG CTGAGCGTTT ACGAAAATTA
     3481 CCCGATTTAT ATGTACGACT TCGAAACCAG CAAGTGGGCA ATCCCGCGTT TTAACCTCGT
     3541 GAATACGTAC TATCAAACCC CGTTTCAGTA CAGCATCGAT GTTATTGTTG ACAACAATTA
     3601 CGATTACAAC AAGCCGGAAA CGATGAAGCA TTACAGCTTC CTGAGCAATG AGCAAGATAC
     3661 CGACCCGCGT ATTAAGTTTA TTGAGAACTT TATTAAGGAC AGCTTCAAGC ATGGCCCGGG
     3721 TGTTTACGTG GCGTATAATA AATCGTTTGA ACAGGGTGTT TTGCGTAAGC TGGCTATGAT
     3781 GTTCCCGAAG TACGCCAAGC CGCTGGCGTA TATTATTCAG AACACCGTCG ATCTGATGGA
     3841 CTTTTTCACC AGCGACACCA AAGTCACCGG CCGTCCTGCC TTTCTGATTT ACCACCCATA
     3901 TTTTAAGGGT AGCTACTCCA TTAAGAAAAC CCAGCCGAGC CTGGACCCGA GCTTCTCTTA
     3961 CAATGACTTG GTTATCAATA AAGGTGATAA AGCGAGCGAA ACGTTCCGCA AGTTTGTCGA
     4021 CGGTCGTATC CCGAAAGCAG CGTGGGACTT GAAAGTGAAA GAAGGTATGC TGAAATACTG
     4081 CGACCGTGAT ACGCTGGCTA TGGTCGTTAT TCTGAAGAAG GTCAAGGAGC TGGTGGAGGC
     4141 ACACAACGGT AAGAACTGAA GAGCTTAGAG ACCGCGTATA CCTTGCTTCC TGT
//