LOCUS       Exported     3187 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3187
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      1225..1272
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3120..3136
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3137..3168
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1153
                     /note="Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s(2)U34."
                     /translation="MKKKVIVGLSGGVDSSVAAYLLIQQGYEVEGLFMRNWDSSANNDILGNQEIDNDVCPQEQDYLDALEVANKLGIKLHRIDFVQEYWEYVFEYFIKEYKKGRTPNPDILCNKYIKFDKFLNHAINELKADYIAMGHYAGVRFNEKTKQYEMIRAVDTNKDQTYFLCQLTQNQLSKTLFPLQSLEKPEIRKIAAEQGLITADKKDSTGICFIGEREFTKFLQNYISNQPGDIVDIKTNEVLGKHIGAMYYTIGQRKGLNLGGMKEPYYVAAKDIDKKIIYVCPASDESYLLSNNAIVTEINWSLDLKQYIDNVEEFECTAKFRYRQPDVKVRLNKIKDNEYKVSYDAIVKAVTPGQEAVFYLNDICLGGGIIDIVE"
                     /label="mnmA"
     primer_bind     complement(1277..1293)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1360..1948)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(2139..2999)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3000..3104)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAAAAAGAAA GTTATTGTGG GCCTGTCCGG
       61 TGGTGTTGAC TCAAGCGTTG CTGCCTATCT GCTGATTCAG CAGGGCTATG AGGTTGAAGG
      121 CCTGTTCATG CGTAATTGGG ACAGCAGCGC CAATAACGAC ATTCTGGGCA ATCAAGAAAT
      181 CGATAATGAT GTTTGTCCGC AGGAGCAGGA CTACCTGGAC GCGCTGGAAG TGGCAAATAA
      241 ATTGGGTATT AAGCTGCACC GCATTGACTT TGTCCAAGAA TACTGGGAAT ACGTTTTTGA
      301 ATACTTCATC AAAGAATACA AAAAGGGTCG CACGCCGAAC CCGGATATCC TGTGTAACAA
      361 ATATATCAAA TTCGATAAGT TTCTCAATCA TGCGATTAAT GAGTTGAAAG CGGATTATAT
      421 TGCTATGGGT CACTATGCCG GCGTTCGTTT CAACGAAAAG ACGAAGCAGT ATGAAATGAT
      481 CCGTGCTGTC GATACCAATA AAGATCAAAC CTACTTCCTG TGCCAACTGA CGCAGAACCA
      541 ACTGTCAAAA ACCCTGTTCC CGCTGCAAAG CCTGGAGAAA CCTGAAATCC GTAAAATCGC
      601 CGCTGAGCAA GGCCTGATTA CCGCAGATAA AAAGGACTCC ACCGGTATTT GCTTCATTGG
      661 CGAGCGTGAG TTCACCAAGT TTCTGCAAAA CTATATCAGC AACCAGCCGG GCGATATCGT
      721 GGATATTAAG ACGAATGAGG TTTTAGGCAA ACATATTGGT GCAATGTACT ACACCATTGG
      781 TCAACGCAAG GGTCTTAATT TGGGTGGTAT GAAGGAGCCG TACTACGTGG CCGCGAAAGA
      841 CATCGACAAA AAGATCATCT ATGTGTGCCC AGCCAGCGAC GAAAGCTATC TGCTGAGCAA
      901 TAATGCGATC GTGACCGAGA TCAATTGGTC CTTGGATCTG AAACAATACA TCGATAATGT
      961 CGAAGAATTC GAATGCACGG CGAAGTTCCG TTATCGCCAG CCGGACGTCA AGGTGCGCCT
     1021 GAATAAGATT AAAGATAACG AGTACAAGGT CAGCTACGAT GCTATCGTTA AAGCGGTCAC
     1081 GCCGGGCCAG GAGGCTGTGT TCTACCTGAA TGATATTTGC TTGGGTGGCG GCATTATTGA
     1141 CATTGTCGAG TGAAGAGCTT AGAGACCCCC CACTGTTAGA TTGAGCTCGC TGAGGTGTCA
     1201 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1261 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1321 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1381 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1441 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1501 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1561 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1621 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1681 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1741 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1801 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1861 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1921 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1981 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     2041 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     2101 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     2161 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2221 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2281 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2341 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2401 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2461 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2521 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2581 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2641 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2701 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2761 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2821 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2881 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2941 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     3001 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     3061 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     3121 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     3181 GTGGGAG
//