LOCUS       Exported     3592 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3592
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1630..1677
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3525..3541
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3542..3573
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1558
                     /label="Mfl413"
                     /note=""
                     /translation="MDNKSLKQTQVENESELFINETPEQTAQRNKEAFVFSNNKAIRSIEKYFKFDTLGAIMKKEIIGGITTFLSLMYILTVNPGLVSGTPSINTGAGNMNYFGIFFATALVSGICCIIMGLFANIPVAMSTSMGMNALVSVNIGISGGLGFEGAMIVTMLSSVVFVTVSLTPLRSFIIKSIPKGIVLAIGIGIGLFIAYVGISSMGWLAQENGIPMASVATLKDNYLPIILGSITLLLILFLAFKKIPGAVAIAILSMSVIAIILASTLPSDSKAISLLGSADLRKWEGWNYDFDGFAWNWTSTFKAFGNAKIWTSPVTYISIFVVMLINFFDATGTMAAFTHQLDQKTNQHKEISQKALVIDSMGTMMASVTGTTPLGVFAESAAGIEQGAKTGLAAVVNGILFLLAIIMFPIFKLIPQPITSAACVYIGIMMIKEAAHVEWDKPEFLVPTFLSILFMIATYEIANGVAMAFIGYSFMMMITAKAKKVHPAVYCLSFLFIIYFIAFAFIQI"
     primer_bind     complement(1682..1698)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(1765..2353)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2544..3404)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3405..3509)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GGATAATAAA TCGTTAAAAC AGACCCAGGT
       61 GGAGAATGAA TCAGAGCTGT TCATTAACGA AACGCCGGAG CAAACTGCAC AGCGCAATAA
      121 GGAAGCGTTT GTGTTCTCGA ATAACAAAGC CATTCGCTCT ATCGAGAAAT ACTTCAAGTT
      181 CGATACTCTG GGTGCTATTA TGAAGAAGGA GATTATTGGT GGTATTACCA CCTTCCTGTC
      241 CCTGATGTAT ATTCTGACCG TTAATCCAGG TCTGGTTAGC GGTACCCCGA GCATTAACAC
      301 TGGTGCGGGC AACATGAATT ATTTCGGCAT TTTCTTCGCG ACCGCACTGG TTAGCGGTAT
      361 CTGCTGTATT ATCATGGGTC TGTTCGCGAA TATTCCGGTC GCCATGTCTA CGAGCATGGG
      421 TATGAATGCA CTGGTGAGCG TTAACATTGG CATTAGCGGT GGCTTGGGCT TCGAAGGTGC
      481 AATGATCGTT ACGATGCTGA GCAGCGTGGT CTTTGTGACC GTTTCCTTGA CCCCGCTGCG
      541 CAGCTTTATC ATCAAAAGCA TTCCGAAAGG TATCGTCCTT GCTATCGGTA TTGGCATTGG
      601 TCTTTTTATC GCGTATGTTG GTATTTCCTC CATGGGTTGG CTGGCGCAGG AGAACGGTAT
      661 TCCGATGGCA TCGGTGGCTA CGCTGAAAGA CAACTATCTG CCGATCATCC TGGGTAGCAT
      721 CACTCTGCTG CTGATCCTGT TCCTGGCGTT CAAGAAAATC CCGGGTGCCG TTGCCATTGC
      781 AATCCTCAGC ATGTCCGTTA TCGCGATTAT TCTGGCGAGC ACCCTGCCGA GCGATTCTAA
      841 AGCGATTAGC CTGTTGGGCA GCGCGGATCT GCGCAAGTGG GAGGGCTGGA ACTACGACTT
      901 TGACGGCTTC GCATGGAATT GGACGAGCAC CTTCAAAGCC TTCGGTAATG CTAAAATTTG
      961 GACGAGCCCG GTTACCTATA TCAGCATCTT CGTTGTCATG TTGATCAACT TTTTTGACGC
     1021 GACCGGCACG ATGGCTGCGT TTACCCACCA ACTGGACCAG AAAACCAATC AGCACAAGGA
     1081 AATCAGCCAA AAAGCTCTGG TTATCGATAG CATGGGTACC ATGATGGCCA GCGTGACCGG
     1141 TACCACCCCG CTGGGTGTGT TCGCTGAGAG CGCGGCGGGC ATTGAGCAAG GCGCTAAGAC
     1201 GGGCCTGGCA GCGGTCGTTA ACGGTATTCT GTTTCTCCTG GCGATCATCA TGTTTCCGAT
     1261 TTTCAAGCTT ATCCCGCAAC CAATCACCAG CGCGGCGTGT GTTTACATCG GCATTATGAT
     1321 GATCAAGGAG GCTGCTCACG TCGAGTGGGA CAAGCCGGAG TTCCTGGTGC CGACGTTTCT
     1381 CAGCATTTTG TTCATGATCG CGACCTATGA GATTGCGAAC GGTGTCGCCA TGGCGTTTAT
     1441 CGGTTACTCC TTCATGATGA TGATCACTGC TAAGGCCAAG AAGGTCCACC CGGCGGTTTA
     1501 CTGTCTCAGC TTTCTGTTCA TCATTTACTT TATCGCGTTT GCATTTATTC AAATTTGAAG
     1561 AGCTTAGAGA CCGGGTGCCT TGCTAATCTA GTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1621 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1681 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1741 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1801 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1861 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1921 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1981 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2041 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2101 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2161 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2221 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2281 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2341 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2401 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2461 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2521 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2581 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2641 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2701 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2761 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2821 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2881 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2941 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3001 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3061 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3121 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3181 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3241 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3301 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3361 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3421 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3481 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3541 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//