LOCUS       Exported     3842 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3842
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3808
                     /translation="MAKERIIGIDLGTTNSVVSIMEGGQPIILENPEGQRTTPSVVAFKNEDIIVGGAAKRQAVTNPNVVISVKSKMGTNEKIDINGKKYTPEQISAEILRYMKKYAEEKVGEKITKAVITVPAYFNDSQRKATKDAGKIAGLDVERIINEPTAAALAYGLEKKDKEEKVLVYDLGGGTFDVSILELADGTFEVLSTSGDNKLGGDNFDTQIINWLIEKIKTESGVDLKNDKMALQRLKDEAEKAKINLSSQLEVEINLPFIAMNENGPVSFSTQFSRTEFDKITKDLVERTSKPVKDALQAAKLSASDIDEVLLVGGSTRIPAVQKIVKELLGKEPNRSINPDEVVAMGAAIQGGVLAGDVTDVLLLDVTPLSLGIETMGGVMTKLIDRNTTIPTEKSQIFSTAVDNQPAVDINVLQGERPMAADNKSLGQFQLTGIKPAPKGTPQIEVTFKIDVNGIVSVIAKDKATNEEKSITISNSGALSDADIEKMIKEAEANAEADEIKRKNIELKHKAESYVAIIESSLTQEGQEIPQEQKEQAEKMVAEVKELIEKEDYEALETKVNELEQMIQMASQFAQAQTAQPEEGNSDSEENK"
                     /label="dnaK"
                     /note="Acts as a chaperone."
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GACAAGGAGT CGGCATATCA CGGTCTCAAA TGGCAAAGGA
     2041 GCGTATCATT GGCATCGACC TGGGTACCAC TAATAGCGTT GTCAGCATCA TGGAAGGCGG
     2101 CCAACCGATC ATCCTTGAGA ACCCGGAGGG TCAGCGTACG ACTCCGTCGG TTGTCGCTTT
     2161 TAAAAATGAA GATATCATCG TCGGCGGCGC TGCGAAACGC CAGGCGGTTA CCAACCCGAA
     2221 CGTTGTTATC TCTGTTAAGA GCAAAATGGG CACGAACGAA AAGATCGATA TTAACGGTAA
     2281 AAAATATACC CCAGAGCAGA TTAGCGCGGA GATTCTCCGT TATATGAAGA AATATGCGGA
     2341 GGAGAAGGTG GGTGAGAAAA TTACCAAGGC GGTTATCACG GTCCCGGCGT ACTTTAATGA
     2401 TAGCCAGCGT AAAGCTACCA AGGACGCTGG CAAGATTGCG GGCCTCGATG TTGAACGCAT
     2461 TATCAATGAA CCGACCGCCG CTGCGCTGGC GTACGGTCTG GAGAAAAAAG ACAAGGAGGA
     2521 AAAGGTACTG GTTTACGACT TGGGCGGTGG TACCTTTGAC GTATCCATCT TAGAGCTGGC
     2581 AGATGGTACC TTTGAGGTTC TGAGCACCAG CGGTGATAAT AAGCTGGGCG GCGATAATTT
     2641 CGACACCCAG ATCATCAACT GGCTGATCGA GAAAATTAAG ACCGAAAGCG GCGTGGATTT
     2701 AAAAAACGAC AAAATGGCGT TGCAACGTCT GAAGGACGAA GCAGAAAAGG CGAAGATTAA
     2761 CCTGAGCAGC CAACTGGAGG TCGAGATCAA TCTGCCGTTT ATCGCCATGA ATGAAAATGG
     2821 TCCGGTCAGC TTTAGCACCC AATTCAGCCG TACCGAATTT GATAAGATCA CTAAAGACCT
     2881 GGTTGAGCGT ACTAGCAAAC CTGTTAAAGA TGCGCTGCAG GCGGCGAAAC TGAGCGCTAG
     2941 CGACATCGAC GAGGTGCTGC TGGTCGGCGG CAGCACCCGT ATTCCGGCAG TTCAGAAAAT
     3001 TGTGAAGGAA CTGTTAGGCA AAGAGCCTAA CCGCTCCATT AATCCTGACG AGGTTGTGGC
     3061 CATGGGTGCT GCTATCCAAG GTGGCGTTCT GGCGGGTGAT GTCACGGACG TGTTGCTCCT
     3121 GGACGTGACC CCGCTCAGCC TGGGCATCGA AACCATGGGT GGCGTTATGA CCAAACTGAT
     3181 TGACCGTAAC ACCACTATCC CTACCGAAAA GTCCCAAATT TTTAGCACCG CAGTTGACAA
     3241 CCAGCCGGCC GTCGATATTA ACGTTCTGCA GGGTGAGCGC CCGATGGCAG CAGATAACAA
     3301 GAGCCTGGGT CAATTTCAGC TGACCGGCAT CAAGCCAGCT CCGAAAGGCA CGCCTCAGAT
     3361 TGAGGTTACT TTCAAAATTG ACGTGAATGG CATTGTTAGC GTTATCGCGA AGGATAAAGC
     3421 GACGAATGAA GAGAAAAGCA TCACCATTAG CAACAGCGGC GCCCTGTCGG ATGCGGACAT
     3481 CGAAAAGATG ATTAAGGAAG CGGAGGCCAA TGCGGAGGCG GACGAGATCA AACGTAAAAA
     3541 CATTGAGCTG AAGCATAAGG CGGAAAGCTA CGTGGCGATC ATTGAGAGCT CCTTGACCCA
     3601 AGAAGGTCAG GAGATTCCAC AGGAACAGAA AGAACAAGCA GAGAAGATGG TTGCTGAGGT
     3661 TAAGGAGCTG ATTGAGAAAG AGGATTACGA GGCGCTGGAA ACCAAAGTGA ATGAGCTGGA
     3721 ACAAATGATT CAAATGGCGA GCCAATTTGC GCAAGCGCAG ACGGCGCAGC CGGAGGAGGG
     3781 TAATTCTGAT AGCGAGGAAA ACAAGTGAAG AGCTTAGAGA CCGTCTCATG CTAGCCACGT
     3841 AT
//