LOCUS       Exported     4199 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4199
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..4165
                     /translation="MEFQQKGEEINALEKYTRDLTQSAKDGKIDPIIGREEEILRVIRILSRKTKNNPVLIGEPGVGKTAVVEGLAQRIVKGDVPSILKGKRILELDMGSLMAGAMYLGDYESRVKAIVNEIKKSEGEIILFIDELHLIVGAGKTGNGGGMDVSNLLKPALARGELKAIGATTLKEYREYIEKDSALERRFQRVMVEEPTVDQTISILRGLKERFETYHGVRIHDNAIVSAANLSNRYIADRFLPDKAIDLIDEASSTIKTELASVPTELDQVNRKVMQLEIEKSALSKEKDDKSKERLAEAVKELEKTKIEQDKLASEWNKQKSQLEKINSFKTTIENLKNELEISQNEGNYKRAGEIQYSLLPSLEKQLSEAEKDTRETLVSEEVTEKDVAEIVAKWTGIPVDRLVESEKTKLLELEKTLEERVKGQNQAIEAVSQAILRSRSGIKDPNKPIGSFLFLGPTGVGKTEVARSLADILFNSNKKMVRIDMSEYMEKHSVSKLIGSPPGYVGYEEGGRLTEAVRRNPYSIVLFDEIEKAHPDVFNILLQVLDEGRITDSLGKTVDFKNTIIILTSNLGSEYILKNGSTSEESKIAIQAELLKHFRPEFLNRIDNVVVFNPLSKENVSEIVTKELNTLSARLENDKNFFLTFDMKAKNKIIDEGYDSQFGARPIKRYIEKNIETLLAQKIISGEIEENIRYTITVENDDFELTKIRN"
                     /label="Mfl418"
                     /note=""
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCAAA TGGAATTTCA
     2041 ACAAAAAGGT GAGGAAATCA ACGCACTGGA GAAATATACC CGCGATCTGA CGCAGTCTGC
     2101 CAAGGATGGT AAAATTGACC CGATCATCGG TCGTGAGGAG GAAATCCTGC GCGTAATCCG
     2161 TATCCTGAGC CGTAAGACCA AGAACAATCC GGTCTTGATC GGTGAACCGG GTGTTGGTAA
     2221 GACCGCGGTG GTTGAAGGCC TGGCACAGCG TATCGTAAAA GGCGACGTGC CGTCCATTCT
     2281 GAAGGGTAAA CGCATTCTGG AATTGGACAT GGGTAGCTTG ATGGCTGGTG CAATGTACCT
     2341 GGGTGATTAC GAAAGCCGCG TCAAAGCAAT TGTTAATGAG ATTAAAAAGA GCGAGGGTGA
     2401 GATCATTCTG TTTATCGACG AGCTGCATTT GATTGTTGGT GCGGGCAAGA CGGGTAACGG
     2461 TGGCGGCATG GATGTGAGCA ACCTGTTGAA ACCGGCACTG GCCCGCGGCG AGCTGAAAGC
     2521 TATCGGTGCG ACCACCTTGA AAGAGTACCG CGAATATATT GAAAAGGATT CCGCGCTGGA
     2581 GCGTCGTTTT CAACGTGTTA TGGTTGAGGA GCCGACCGTG GACCAGACCA TCAGCATTCT
     2641 GCGCGGTTTG AAAGAGCGTT TTGAAACGTA CCATGGTGTT CGCATCCACG ATAACGCGAT
     2701 TGTGTCAGCG GCAAATCTGA GCAACCGTTA TATCGCGGAT CGCTTTCTGC CGGATAAGGC
     2761 TATTGATCTG ATTGATGAGG CCTCCAGCAC CATCAAAACC GAGCTGGCAA GCGTCCCGAC
     2821 CGAACTGGAC CAGGTTAACC GTAAAGTTAT GCAACTGGAA ATTGAAAAAA GCGCCCTGAG
     2881 CAAGGAGAAA GACGACAAAA GCAAAGAGCG CCTGGCCGAA GCTGTTAAAG AGCTGGAAAA
     2941 GACCAAGATT GAACAGGACA AGTTAGCGTC TGAGTGGAAC AAACAGAAAT CACAACTGGA
     3001 AAAAATCAAT TCGTTTAAAA CCACTATCGA AAACCTGAAA AATGAGCTGG AAATCAGCCA
     3061 GAATGAGGGC AATTACAAAC GTGCGGGTGA GATCCAGTAC AGCCTGCTGC CGAGCCTGGA
     3121 GAAACAGCTG TCTGAGGCTG AGAAAGATAC CCGTGAAACC TTAGTCTCTG AAGAGGTTAC
     3181 CGAGAAGGAC GTTGCCGAGA TCGTGGCGAA ATGGACGGGC ATTCCAGTTG ATCGCCTGGT
     3241 CGAGTCCGAA AAAACCAAAC TGCTGGAGCT CGAAAAGACG CTGGAAGAAC GTGTCAAGGG
     3301 CCAGAACCAA GCTATTGAAG CAGTCAGCCA GGCTATTCTG CGTTCTCGTA GCGGCATCAA
     3361 GGATCCAAAT AAGCCGATCG GTAGCTTCTT ATTTCTTGGT CCTACCGGCG TTGGTAAAAC
     3421 CGAAGTGGCA CGTAGCCTGG CGGACATCCT GTTCAATTCG AACAAGAAGA TGGTCAGAAT
     3481 CGACATGAGC GAATACATGG AGAAGCATAG CGTAAGCAAG CTGATTGGTA GCCCGCCGGG
     3541 CTACGTGGGC TACGAGGAGG GCGGCCGCCT GACTGAGGCC GTTCGTCGCA ATCCGTATTC
     3601 GATTGTTCTG TTCGATGAAA TCGAAAAGGC GCATCCGGAC GTGTTTAACA TTCTGCTCCA
     3661 GGTGTTGGAT GAAGGTCGCA TTACGGACAG CTTAGGCAAA ACGGTCGATT TCAAGAATAC
     3721 GATCATTATC TTAACGAGCA ATCTCGGCAG CGAGTACATT CTGAAGAACG GTAGCACCAG
     3781 CGAAGAGTCT AAGATCGCGA TTCAAGCTGA ACTGCTGAAA CACTTCCGCC CGGAATTTCT
     3841 GAACCGTATC GACAACGTTG TAGTTTTCAA CCCACTGAGC AAGGAAAATG TGAGCGAAAT
     3901 CGTGACGAAG GAGCTGAATA CCCTGAGCGC TCGTCTGGAA AATGATAAGA ACTTTTTCCT
     3961 GACGTTCGAC ATGAAAGCCA AAAACAAGAT TATTGACGAA GGCTACGATA GCCAGTTCGG
     4021 TGCGCGTCCA ATCAAGCGCT ATATTGAGAA AAACATTGAA ACGCTGCTGG CGCAGAAAAT
     4081 TATTAGCGGT GAAATTGAAG AAAATATTCG TTACACCATT ACGGTGGAAA ACGACGATTT
     4141 TGAGCTGACC AAGATTCGTA ACTGAAGAGC TTAGAGACCC TGGAATCAGC CTTGTGTCT
//