LOCUS       Exported     3896 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3896
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..3862
                     /label="Mfl422"
                     /note=""
                     /translation="MKKKKEISNSRAFLYLFFKYYKQDFKKNWWVIPNFIFWASMLFLAPLLTNQINLALSLTDAEKAASSGFWLIRFWGLETTELIIISVALLLFNLFNSFLFNWWSNTFAKQIETHLRNEILEKLIRQDISYYSDKKIGELLTNVISDAQVVGNQAVDIPMNVASAVIQSLIGLSLTFILEWRIALVGFVIFVLILIAFFGCYIALVKKWEKVRLTLEKTNGNVIDRVISIRLIKSSGTEDYETQYFKDKHVDYFDQGKPIGKWLSLFSVIVFAGDFLLLFTAPVFAAVFFGGDSDTARMQAFFQVFPAFVMAQGMLTGPIITLFVISGGAAMAGVSSSRILKTLNAESILDFHYHEGKEIKDLKGDIVFKDVKFRYPEKPENLILPNFNFTLQYGKSYAFVGETGSGKSTVAKLLLRFYDPTEGQILINNKDDLKDLNLSHYLDHVGYVEQEPQILFGTVLENIKYGKFDATDDDAIEACKKAELHALVMSWPEGYQTVLGERGFMLSGGQKQRLVIARMILKNPELLILDEATSALDNIVEKEIQNKLNQLMKGRTSVTIAHKLSTIKNVDHIIVLGANGAGIVQEGTFNELTKKEGHFKNLYEAGKIEH"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGTATGT ATAGCGACCG CGGTCTCAAA TGAAGAAAAA
     2041 GAAGGAAATT AGCAACTCGC GTGCCTTTCT GTACCTGTTT TTCAAATACT ACAAGCAAGA
     2101 CTTCAAAAAG AATTGGTGGG TAATTCCGAA CTTCATCTTC TGGGCAAGCA TGTTGTTCCT
     2161 GGCGCCGCTG CTGACCAATC AGATCAATTT GGCCCTGAGC TTGACCGACG CAGAAAAAGC
     2221 AGCGAGCTCT GGTTTCTGGC TGATTCGCTT CTGGGGTTTG GAAACGACCG AACTGATCAT
     2281 TATCAGCGTG GCTCTGCTGT TGTTTAATCT GTTCAATTCA TTCCTGTTCA ACTGGTGGAG
     2341 CAACACGTTT GCTAAACAAA TCGAAACCCA TCTGCGTAAC GAAATCCTGG AGAAGCTGAT
     2401 CCGCCAAGAC ATTTCGTACT ACAGCGATAA GAAGATTGGC GAGCTGCTTA CTAATGTTAT
     2461 TAGCGACGCG CAAGTTGTTG GTAACCAAGC AGTTGACATC CCGATGAATG TAGCCAGCGC
     2521 GGTTATCCAA TCTCTGATCG GTCTGTCCCT GACGTTCATC CTGGAATGGC GTATTGCGCT
     2581 GGTTGGCTTC GTTATTTTCG TGCTGATTCT GATCGCTTTC TTTGGCTGCT ATATCGCATT
     2641 GGTTAAAAAA TGGGAGAAGG TTAGACTGAC GCTGGAAAAG ACCAACGGCA ATGTTATCGA
     2701 CCGTGTCATT AGCATCCGTC TGATCAAGAG CTCCGGTACC GAAGATTATG AAACCCAATA
     2761 CTTCAAAGAC AAACACGTGG ACTATTTTGA CCAAGGTAAA CCGATTGGTA AATGGCTGAG
     2821 CCTGTTTTCC GTCATCGTTT TTGCCGGCGA CTTTCTGTTA TTGTTTACCG CGCCAGTGTT
     2881 TGCTGCAGTG TTTTTCGGTG GTGATAGCGA CACCGCACGT ATGCAGGCAT TCTTTCAGGT
     2941 CTTTCCGGCA TTCGTGATGG CACAAGGCAT GCTGACTGGT CCGATTATCA CGCTGTTTGT
     3001 GATTAGCGGT GGCGCCGCTA TGGCTGGCGT TAGCAGCAGC CGTATCCTGA AAACTTTGAA
     3061 TGCGGAGAGC ATTCTGGATT TTCACTATCA CGAGGGCAAG GAAATTAAAG ATCTGAAAGG
     3121 TGATATCGTG TTTAAAGATG TGAAATTCAG ATACCCGGAA AAGCCTGAAA ATCTGATTCT
     3181 GCCGAATTTC AACTTTACCC TCCAGTATGG TAAGAGCTAC GCCTTTGTCG GTGAAACCGG
     3241 CAGCGGTAAG AGCACCGTTG CAAAACTGTT GCTGCGTTTC TACGACCCGA CGGAGGGCCA
     3301 AATCTTGATC AACAATAAAG ACGACTTGAA AGACCTGAAC CTGAGCCACT ACCTGGACCA
     3361 TGTTGGTTAT GTTGAGCAAG AACCGCAAAT TCTGTTCGGC ACCGTTCTGG AGAACATCAA
     3421 GTATGGTAAA TTCGATGCCA CGGACGACGA TGCAATCGAG GCGTGTAAAA AGGCAGAACT
     3481 GCACGCGCTG GTGATGAGCT GGCCGGAAGG TTATCAAACG GTTCTGGGTG AGCGCGGTTT
     3541 CATGCTGAGC GGTGGTCAGA AACAACGTTT AGTGATCGCC CGTATGATTC TGAAGAATCC
     3601 AGAGCTGCTG ATCTTAGATG AGGCGACGAG CGCTCTGGAT AACATCGTGG AAAAAGAGAT
     3661 TCAGAATAAA CTGAATCAAC TGATGAAAGG CCGTACGAGC GTGACGATCG CACACAAACT
     3721 GAGCACCATT AAGAACGTGG ACCACATCAT CGTGCTGGGT GCGAATGGTG CCGGTATCGT
     3781 TCAGGAGGGC ACCTTCAACG AGTTAACGAA AAAGGAGGGT CATTTTAAAA ACCTGTACGA
     3841 GGCGGGTAAG ATCGAACATT GAAGAGCTTA GAGACCCAGT CCTTGCCAAT CGAAGT
//