LOCUS       Exported     3908 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3908
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3874
                     /label="Mfl423"
                     /note=""
                     /translation="MKTNKKTSQNAEFFKIISKYYLKYWWITIILALTILSFCLTRASIPLLTQQITLAIKSENKVVLNDAEQALFWGWSSTTIIIVAVVMILIDAIGTFIFNYFSYILGRKIEIDLRNKILEKLVRQDISYYSDKKIGEILTSVVADTQNLGDGAVRIPTNIGISLSQFVVAFTMTFILAWKIAIFGSIIYFLLLALYFVFYIQTVKKYAVVREVYEEVNGNVTDRVGTIRLIKSTGTEDYEKAYLEKQQDINYKAHKPAILNLSFLMTTVYAGSMILQFAIPIIAGIIYSIKGDTAASATFFSVTFPAYMINQASLIATYSSLMGITFSLAVAGVASVKVSNLLKDESRLDPHYTDGIIVDEIKGDIVFKDIEFRYPEKPEKLILPKFNFTFEEGKSYAFVGETGSGKSTIARLLLRFYDPSNGEIIINKDQNLKDLNLSSYLRHVGYVEQEPQILFGDVYENVKYGRFDATNEEVIEACKKAEIHKLISSWPEGYDTILGERGFMLSGGQKQRLVIARMILKNPQILILDEATSALDNIVEKEIQAKLNELMKGRTSFTIAHRLSTIKNVDHIIVLGGNAKGIVQQGTFKELVKVEGHFKNLYEAGLLEEQQQNH"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGTATGT ATAGCGACCG CGGTCTCAAA TGAAAACTAA
     2041 TAAGAAAACT AGCCAGAATG CGGAATTCTT CAAGATTATC TCTAAGTATT ATCTGAAATA
     2101 CTGGTGGATT ACGATTATCC TGGCACTGAC GATTCTGAGC TTTTGCTTGA CTCGTGCGAG
     2161 CATCCCACTG TTGACCCAAC AAATCACTCT TGCCATTAAG AGCGAGAACA AGGTTGTCCT
     2221 GAATGACGCG GAGCAAGCAC TGTTCTGGGG TTGGAGCTCT ACCACGATTA TTATCGTAGC
     2281 AGTTGTTATG ATTCTGATTG ACGCGATTGG TACCTTCATC TTTAACTACT TCAGCTACAT
     2341 CCTGGGCCGT AAGATTGAAA TTGATCTGCG CAATAAAATT CTGGAGAAAC TGGTGCGTCA
     2401 AGACATTAGC TATTACAGCG ATAAGAAAAT CGGTGAAATT CTGACCAGCG TTGTTGCGGA
     2461 TACGCAAAAC CTGGGCGACG GTGCAGTTCG TATTCCAACC AATATTGGTA TCTCCCTGAG
     2521 CCAATTCGTG GTGGCCTTCA CGATGACGTT CATCCTGGCG TGGAAAATTG CGATTTTTGG
     2581 TAGCATTATT TACTTCTTGT TGCTGGCGTT ATACTTCGTG TTTTACATTC AGACGGTCAA
     2641 GAAGTACGCG GTCGTGCGCG AGGTCTACGA AGAAGTTAAC GGTAATGTGA CGGACCGCGT
     2701 CGGTACCATT CGCCTGATTA AAAGCACGGG TACCGAGGAC TATGAGAAAG CATACCTGGA
     2761 AAAACAGCAG GATATCAACT ACAAGGCACA CAAACCGGCC ATTTTAAATT TGTCCTTTCT
     2821 GATGACCACC GTGTACGCGG GCAGCATGAT TCTGCAGTTT GCGATTCCGA TCATTGCGGG
     2881 TATTATCTAT AGCATTAAAG GTGACACGGC CGCGAGCGCA ACCTTTTTCT CTGTCACCTT
     2941 TCCGGCCTAT ATGATTAATC AGGCGAGCCT GATTGCAACC TACAGCTCCC TGATGGGTAT
     3001 TACGTTTAGC CTGGCGGTTG CCGGCGTGGC CAGCGTCAAA GTCTCTAATC TGCTGAAAGA
     3061 CGAAAGCCGT CTGGATCCGC ACTATACGGA TGGTATTATT GTTGACGAAA TTAAAGGTGA
     3121 CATTGTTTTT AAGGACATCG AGTTCCGTTA CCCGGAAAAG CCGGAAAAGC TGATCTTGCC
     3181 GAAGTTCAAT TTTACCTTCG AGGAGGGTAA AAGCTATGCC TTTGTGGGCG AAACGGGTAG
     3241 CGGCAAAAGC ACCATTGCGC GTCTGCTGTT GCGCTTCTAC GATCCTAGCA ACGGTGAAAT
     3301 CATTATTAAT AAAGACCAGA ATCTGAAAGA CCTGAACCTC AGCAGCTATC TGCGCCACGT
     3361 CGGTTACGTC GAGCAGGAGC CTCAAATCCT GTTCGGTGAT GTCTACGAGA ATGTCAAATA
     3421 TGGCCGCTTC GACGCGACCA ACGAAGAAGT GATTGAGGCA TGCAAGAAGG CTGAAATTCA
     3481 CAAATTAATT AGCAGCTGGC CGGAGGGTTA CGACACCATC TTGGGTGAAC GTGGTTTTAT
     3541 GCTGAGCGGC GGTCAGAAAC AACGCCTGGT GATCGCACGT ATGATTTTAA AGAACCCTCA
     3601 GATCTTAATC CTTGATGAGG CGACGTCTGC ACTGGATAAC ATCGTGGAGA AAGAGATTCA
     3661 GGCGAAGCTG AATGAGCTGA TGAAAGGTCG TACGAGCTTC ACTATTGCTC ACCGTCTGAG
     3721 CACCATCAAA AATGTTGATC ATATTATCGT CCTGGGCGGT AATGCGAAAG GTATTGTTCA
     3781 GCAAGGCACT TTTAAGGAGC TGGTCAAAGT TGAGGGCCAC TTCAAGAATC TGTACGAGGC
     3841 CGGTCTGCTT GAGGAACAGC AGCAAAATCA TTGAAGAGCT TAGAGACCGC CGTGTAAAAT
     3901 GACGCATT
//