LOCUS       Exported     3502 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3502
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1540..1587
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3435..3451
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3452..3483
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1468
                     /note=""
                     /translation="MSDILFRCPKCGEEITKDSFKNHNSNWTVIENYLSELKQKQENEIRVQLKNELIAQMNDKQIAEISLVKQEMLNNFNKQLESLNISINEKDNLINKLNEQKALEIDKERNAVINEFNEKIKTYELNIQKQQNMIDQLNQQKIAELNEAKSNLLNEFNDVKNSLEIKLANKEVEIKALDQKLNLEIEKNKEVLINQFSQEINNLKLELNNKKIELENFAIQKEAESKTKELTLINDYDKRIADLVQANREFKIINSKRKGENFEHEVYEDLVKAFSLFDGIEKITTGEKKADYLQTIKNHKREEIGKIVYEVKNAEWSNTWIPKLSTDAASNNTKYGILIATSFNDKYPGIPFMRSDEYENIWITDSESFVFVGQIVRRLVEFENEYNQKIKTVTKASDNELLKELEKQKAELNEYWTIQFPTAYKKMQKELEDLDKVADSLERNSSRIKKSINVINQQFFNKVQKGLSSILGEMKAEYL"
                     /label="Mfl427"
     primer_bind     complement(1592..1608)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1675..2263)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2454..3314)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3315..3419)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCGCTCG GATAATACTC GGTCTCAAAT GAGCGACATT CTGTTTCGTT GCCCGAAATG
       61 TGGCGAAGAA ATTACCAAGG ATAGCTTTAA GAACCACAAC AGCAATTGGA CGGTCATCGA
      121 GAACTACCTG AGCGAGCTGA AACAGAAGCA AGAAAACGAA ATCCGCGTCC AGCTGAAGAA
      181 CGAGCTGATT GCACAAATGA ACGATAAACA GATCGCGGAG ATTTCCCTGG TCAAGCAGGA
      241 GATGCTGAAC AACTTCAACA AGCAACTGGA AAGCCTGAAC ATCTCCATCA ACGAGAAAGA
      301 CAACCTGATC AACAAATTAA ATGAGCAGAA GGCTCTGGAA ATTGACAAAG AGCGTAATGC
      361 AGTTATCAAT GAATTCAATG AAAAAATTAA AACCTACGAA CTGAATATCC AGAAACAGCA
      421 GAACATGATC GACCAGCTGA ACCAGCAGAA AATCGCCGAG TTAAATGAGG CAAAGAGCAA
      481 CCTGTTGAAC GAGTTTAACG ATGTTAAGAA TAGCTTGGAG ATTAAGCTGG CGAATAAAGA
      541 AGTTGAGATT AAGGCCCTGG ACCAAAAGCT GAATTTGGAA ATCGAAAAGA ACAAGGAGGT
      601 GCTGATTAAT CAATTCAGCC AGGAGATCAA TAACTTGAAG CTGGAGCTGA ATAATAAGAA
      661 AATTGAACTG GAGAACTTTG CCATCCAAAA GGAAGCGGAG AGCAAAACCA AGGAACTGAC
      721 CTTAATTAAT GATTATGACA AGCGTATCGC GGACCTGGTC CAGGCAAATC GCGAATTTAA
      781 AATCATCAAT AGCAAACGCA AAGGTGAGAA TTTCGAGCAT GAGGTTTACG AGGACCTGGT
      841 CAAAGCTTTT TCGCTGTTTG ATGGCATCGA GAAGATCACC ACGGGTGAGA AAAAGGCGGA
      901 TTATCTGCAA ACCATTAAAA ACCACAAACG TGAGGAAATT GGTAAAATTG TCTATGAGGT
      961 CAAAAACGCG GAGTGGAGCA ATACTTGGAT TCCAAAACTG AGCACCGATG CGGCGAGCAA
     1021 TAACACCAAA TATGGCATCC TGATCGCGAC CAGCTTTAAC GACAAGTATC CGGGCATCCC
     1081 GTTCATGCGC TCTGATGAGT ACGAAAACAT TTGGATTACG GATTCTGAAA GCTTTGTTTT
     1141 TGTCGGCCAG ATCGTGCGTC GTCTGGTCGA GTTTGAGAAC GAGTACAATC AAAAGATTAA
     1201 AACTGTCACT AAAGCGTCTG ACAATGAACT GCTGAAAGAA TTGGAAAAAC AAAAAGCTGA
     1261 ACTGAACGAG TATTGGACGA TCCAGTTCCC GACGGCATAT AAAAAGATGC AGAAAGAACT
     1321 CGAGGACCTG GATAAAGTGG CCGACAGCCT GGAACGCAAC AGCAGCCGTA TTAAAAAGAG
     1381 CATTAATGTC ATCAACCAGC AGTTCTTCAA CAAAGTGCAG AAGGGTCTGA GCAGCATTTT
     1441 GGGTGAAATG AAAGCAGAGT ATCTGTGAAG AGCTTAGAGA CCCCAGTATC GGCAGCTAAT
     1501 CTCGCTGAGG TGTCAATCGT CGGAGCCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC
     1561 TCTAAACGGG TCTTGAGGGG TTTTTTGCAT GGTCATAGCT GTTTCCTGAG AGCTTGGCAG
     1621 GTGATGACAC ACATTAACAA ATTTCGTGAG GAGTCTCCAG AAGAATGCCA TTAATTTCCA
     1681 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA
     1741 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC
     1801 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC
     1861 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT
     1921 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG
     1981 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG
     2041 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA
     2101 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG
     2161 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT
     2221 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGGCC TACTATTAGC
     2281 AACAACGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT
     2341 TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA
     2401 AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA
     2461 TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC
     2521 TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT
     2581 GCAATGATAC CGCGAGAACC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA
     2641 GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT
     2701 AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT
     2761 GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC
     2821 GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC
     2881 TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT
     2941 ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT
     3001 GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC
     3061 CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT
     3121 GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG
     3181 ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT
     3241 GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA
     3301 TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT
     3361 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC
     3421 ACCTGCACCA GTCAGTAAAA CGACGGCCAG TAGTCAAAAG CCTCCGACCG GAGGCTTTTG
     3481 ACTTGGTCAG GTGGAGTGGG AG
//