LOCUS       Exported     3608 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3608
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3574
                     /label="Mfl431"
                     /note=""
                     /translation="MKFTKENIVFLLENVGGSENIEKISHCISRMRFKLVDITKANTEEIKKQKWCKGVLIVGGEYQVVIGTEIESFYKMFLKTTGINENANDELKTKKQKMTFIMFVSSIFSPIMILLVVYGMWEMVRTPFFLSSNSTEIPTLIEINSFMELISKGLSWFIVMAVCWSTFRVMGGTPIIGLAIGAILISPMLTPLSALDLDGGKTIIQAMNEHGWKIFGNAVFPWKVSFEGLVIPMIFVGLLGVFIERGMNKVNLGGARMLIQPMVVIAGTVIVTMFTIAPAGLIITNYMSISFNFLMTHNITKYIFSPLIGMLYAPMVVFGLHRTITPILMQDILKFNGSFIMGLMIISNICLAVGCLTFGRLNKNCKEVKNIAYSNGISAFIAGVTEPAIYSISLKYLFPLIASAIGTYFGCLLYTSAGVWTNAAPFGILGIIGFASTIPQGINVEQWAGGSIVWGTLSVILGISITIFMTIVLSQVKYFKERTNKILFDEYGFTPELKQKSIFKFKGNKNGKIK"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGATATG TAGACACCGC CGGTCTCAAA TGAAGTTTAC
     2041 CAAAGAAAAC ATCGTTTTTC TGCTGGAAAA CGTTGGCGGT AGCGAGAATA TCGAAAAGAT
     2101 CTCCCATTGT ATTAGCCGCA TGCGTTTCAA ACTGGTGGAC ATTACCAAGG CCAACACCGA
     2161 AGAGATCAAG AAGCAAAAGT GGTGTAAAGG TGTGCTGATC GTTGGCGGCG AATATCAAGT
     2221 GGTGATCGGC ACGGAGATCG AATCCTTCTA TAAGATGTTT CTGAAAACCA CCGGCATCAA
     2281 CGAAAATGCG AATGATGAGT TAAAGACGAA GAAGCAAAAA ATGACCTTCA TTATGTTTGT
     2341 GAGCAGCATC TTTAGCCCGA TTATGATCCT GCTGGTCGTT TATGGCATGT GGGAAATGGT
     2401 CCGTACCCCA TTCTTCCTGA GCTCCAACTC CACCGAGATT CCGACCTTGA TCGAGATCAA
     2461 CAGCTTCATG GAACTGATTT CCAAGGGCCT GAGCTGGTTC ATCGTGATGG CGGTGTGCTG
     2521 GAGCACCTTC CGCGTGATGG GTGGCACGCC AATTATTGGT CTGGCGATCG GCGCCATCCT
     2581 GATTAGCCCG ATGCTGACGC CGTTGTCCGC GCTGGACTTG GATGGCGGCA AGACCATCAT
     2641 CCAGGCCATG AACGAGCATG GTTGGAAGAT TTTTGGTAAC GCGGTTTTTC CGTGGAAGGT
     2701 TAGCTTCGAA GGCCTGGTCA TCCCTATGAT CTTCGTCGGT CTGCTGGGTG TTTTCATTGA
     2761 GCGTGGCATG AACAAAGTCA ATCTGGGTGG TGCCCGCATG CTGATTCAAC CGATGGTCGT
     2821 CATTGCGGGC ACGGTGATTG TCACTATGTT CACGATTGCG CCTGCCGGTC TGATTATTAC
     2881 CAACTATATG AGCATCTCCT TCAACTTTCT GATGACGCAT AACATCACCA AATATATTTT
     2941 TAGCCCGCTG ATTGGCATGC TGTATGCACC GATGGTGGTT TTTGGCCTGC ATCGTACCAT
     3001 TACGCCGATT CTGATGCAGG ACATTCTGAA ATTTAACGGT AGCTTCATCA TGGGCCTGAT
     3061 GATCATCAGC AACATTTGTC TGGCGGTTGG TTGTCTGACG TTTGGTCGTC TGAACAAGAA
     3121 CTGTAAAGAG GTTAAGAATA TCGCTTACAG CAATGGTATC AGCGCGTTCA TTGCGGGTGT
     3181 GACCGAGCCG GCAATTTATA GCATCAGCCT GAAATATCTG TTCCCGCTGA TTGCAAGCGC
     3241 AATCGGCACG TATTTCGGCT GCCTGCTGTA CACGAGCGCC GGTGTATGGA CCAACGCGGC
     3301 GCCGTTCGGC ATTCTGGGTA TCATCGGCTT TGCCAGCACC ATCCCGCAAG GCATCAATGT
     3361 GGAACAATGG GCCGGTGGTA GCATTGTGTG GGGCACCTTA TCTGTCATTC TGGGTATCTC
     3421 GATTACGATC TTTATGACGA TTGTTCTGTC CCAGGTCAAA TATTTTAAAG AGCGCACCAA
     3481 CAAAATTCTG TTTGATGAAT ATGGTTTCAC CCCGGAGCTG AAGCAGAAAA GCATTTTTAA
     3541 GTTCAAGGGT AACAAGAACG GTAAAATTAA GTGAAGAGCT TAGAGACCGG TTACAATAAG
     3601 GGCTCCGT
//