LOCUS       Exported     3655 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3655
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1693..1740
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3588..3604
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3605..3636
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..1621
                     /label="Mfl458"
                     /note=""
                     /translation="MKKLLLVLSSLVVTSSASTIAVSCSTKAEKQNIDSIERFLTIILHSKENDQEPWTNDELEAELVNQKVDEAGGISVKASDEIKEGTITNKQQSIVFTGNGTSKNGYKYTGSITLIYNFGGNKPAPKKKISQQELATTITALQSFLDTIYFKSENAVRSTFVPNANIPGTPAEGLVFKTITVTILDSTQPTDPSIPKLTKFEISGTIGIKNNDDGYYEFEDEVTEEDKKFTCQGTIVDAVIIEDTVITEATKSINDWIKNEKFSDYNDFHDKLLTQQKEILANEQIAIDSITDSGITENETNIDSIYWTNQVSVNLKPNVGYEFSKEVIESEQYKIKTEIEILKIQSNPLKWHVLTSKGNKYIEPQFIQFDKNVNTTSKIQKEYLSNQQITNEWNQDNDKLQQKEFVKSLNIFTGDDKDKKISESVNSYYFTNTTAFNYGSDASESLFKVDEVLFFDKTQENLNNNIESVLGQTKSIDEFVSSSNALTRTTIENTQNTSYYAVFLTKNSNGTYKAANRTLNVLLQEIAVVD"
     primer_bind     complement(1745..1761)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1828..2416)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2607..3467)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(3468..3572)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAAAAAGCTG TTGCTGGTAC TGAGCAGCCT
       61 GGTCGTGACC AGCTCTGCGT CCACGATCGC GGTTTCCTGC AGCACGAAAG CTGAAAAACA
      121 GAACATTGAC AGCATTGAGC GTTTTCTGAC CATCATTCTG CATAGCAAGG AGAATGACCA
      181 GGAACCGTGG ACGAACGACG AGCTGGAGGC GGAACTGGTT AACCAAAAGG TGGACGAAGC
      241 CGGTGGTATT TCTGTCAAGG CGAGCGACGA AATTAAGGAG GGTACCATCA CTAACAAGCA
      301 GCAAAGCATC GTTTTCACGG GTAATGGTAC CAGCAAGAAC GGCTATAAAT ACACCGGCTC
      361 GATTACGCTG ATTTACAATT TCGGTGGTAA CAAACCGGCG CCGAAGAAGA AGATTAGCCA
      421 GCAAGAACTG GCGACTACGA TCACGGCTCT GCAAAGCTTT CTGGACACGA TTTATTTTAA
      481 AAGCGAGAAT GCGGTCAGAA GCACCTTTGT GCCGAACGCT AATATTCCGG GTACCCCGGC
      541 AGAAGGTTTG GTTTTCAAAA CTATCACGGT CACCATCCTG GATTCGACGC AACCAACGGA
      601 CCCGAGCATC CCGAAGCTGA CCAAGTTTGA AATCTCGGGT ACCATTGGTA TCAAAAACAA
      661 TGACGACGGC TATTACGAGT TCGAGGATGA AGTTACCGAG GAGGACAAAA AGTTTACGTG
      721 CCAAGGCACC ATTGTGGACG CAGTGATTAT TGAGGACACT GTGATTACCG AGGCCACCAA
      781 GAGCATCAAT GACTGGATTA AGAACGAAAA GTTCTCCGAC TATAACGATT TCCACGACAA
      841 GTTACTGACC CAACAAAAAG AGATCCTGGC CAACGAACAA ATTGCCATCG ACAGCATCAC
      901 CGATTCCGGC ATCACGGAAA ATGAAACGAA CATCGATAGC ATCTACTGGA CGAACCAAGT
      961 GAGCGTGAAT CTGAAACCTA ACGTCGGTTA CGAATTTAGC AAAGAGGTGA TCGAGAGCGA
     1021 GCAGTATAAG ATTAAGACCG AGATTGAGAT TCTGAAGATT CAGAGCAATC CGCTGAAGTG
     1081 GCACGTGCTC ACCAGCAAAG GTAATAAGTA TATTGAACCG CAATTCATCC AGTTTGATAA
     1141 GAATGTTAAC ACCACGAGCA AGATTCAGAA AGAGTACCTG TCCAATCAAC AAATTACCAA
     1201 CGAATGGAAT CAAGACAACG ACAAACTGCA GCAGAAAGAG TTTGTTAAAT CTTTAAACAT
     1261 CTTCACGGGT GATGATAAAG ACAAGAAGAT TTCGGAGAGC GTTAATTCTT ACTACTTCAC
     1321 TAACACTACC GCGTTTAATT ACGGCTCCGA TGCAAGCGAA AGCCTGTTCA AAGTTGACGA
     1381 AGTGCTGTTT TTCGATAAAA CCCAAGAAAA TCTGAATAAT AATATTGAAA GCGTGCTGGG
     1441 CCAGACGAAG TCTATCGACG AGTTCGTAAG CAGCAGCAAC GCATTGACGC GTACCACTAT
     1501 CGAGAACACC CAGAACACGT CGTATTATGC GGTTTTTCTG ACCAAAAATA GCAATGGTAC
     1561 CTACAAGGCG GCTAACCGCA CCCTCAATGT TCTGTTGCAA GAGATTGCCG TCGTTGATTG
     1621 AAGAGCTTAG AGACCGCGGT CGCTATACAT ACCTTCGCTG AGGTGTCAAT CGTCGGAGCC
     1681 GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG
     1741 CATGGTCATA GCTGTTTCCT GAGAGCTTGG CAGGTGATGA CACACATTAA CAAATTTCGT
     1801 GAGGAGTCTC CAGAAGAATG CCATTAATTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1861 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     1921 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     1981 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA
     2041 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2101 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2161 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2221 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG
     2281 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2341 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2401 AAAAAAAGGA TCTCAAGAAG GCCTACTATT AGCAACAACG ATCCTTTGAT CTTTTCTACG
     2461 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA
     2521 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT
     2581 ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA
     2641 GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG
     2701 ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA ACCACGCTCA
     2761 CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT
     2821 CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT
     2881 AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA
     2941 CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA
     3001 TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA
     3061 AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT
     3121 GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA
     3181 GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG
     3241 CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC
     3301 TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA
     3361 TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT
     3421 GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT
     3481 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT
     3541 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACCTGCA CCAGTCAGTA AAACGACGGC
     3601 CAGTAGTCAA AAGCCTCCGA CCGGAGGCTT TTGACTTGGT CAGGTGGAGT GGGAG
//