LOCUS       Exported     4084 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4084
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      2122..2169
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     4017..4033
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      4034..4065
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..2050
                     /label="Mfl459"
                     /note=""
                     /translation="MKKLLLILSSFAVVGTSSMAVISCSKDPVEPPVDPAEKEKEAIRQLIRQFESEVQTKFSTIVSSPMATRSTLLDTEASNNGLIFFQKDNLKSIYDQASAGTGGGETGPETSTYANEQSVKFYDVLTPDQQKDLTSNVESLLSPAKAMVDLRNAISTSTYSVLIGSFGSRWIDDIKFDYENAEMTYGNTGADGEGFISGMNLNFSSTYRYRDAEQATVTNTVRGTVVITVSDNGEIIAAINKISNELAGDMLKEANSKVYIDFETLKALEPSLTIQDVLTANTTTYKSAINKYNQSFAKSMTSTIENKYFRNTSSAVVNAINVSYYNENDIIRQEQTNHFSNTGKISVEFDQGAWPEWNTFFGKVKTTDQIQLDGSRVNGDQVLYKSLENQWQQVLQNYNNKLKSEYDRRVQTSVIQENERTPDLLAMSISSETITNKGLTISLRNGYTQRLSDVGFTYSIAIDNKATSASEVNSPQTSKIFSAYYKGIEQMLNTFHKFYGISESDIWSSPNASLQQKQNKFKMSGKTGVKKEDGTDFNIWDYWKETGAMNGTINGTNFANALSLNTGVEAQQIKEQNLISKIEGLTYLNLTYSDVNRNSSQSNYRGVRSATIADSSYTGNNGTMLMSGLSMSTRNNSALTFGISTDLLDVSFGKGQRFDIQSHGRYTFIERVQ"
     primer_bind     complement(2174..2190)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2257..2845)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(3036..3896)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3897..4001)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAATAAGACG ACGACCCTCG GGTCTCAAAT GAAAAAGCTG CTGCTGATTC TGAGCAGCTT
       61 CGCAGTCGTC GGCACCAGCA GCATGGCCGT GATTAGCTGT AGCAAAGATC CGGTCGAACC
      121 TCCAGTTGAC CCGGCGGAAA AAGAGAAGGA AGCCATTCGC CAGTTGATTC GTCAATTCGA
      181 GAGCGAAGTG CAAACCAAGT TTAGCACCAT TGTCAGCAGC CCTATGGCGA CCCGCTCCAC
      241 GCTGCTGGAC ACCGAGGCAA GCAACAACGG CCTGATCTTT TTCCAGAAGG ATAATCTGAA
      301 AAGCATCTAC GATCAAGCAT CCGCGGGTAC CGGTGGTGGT GAAACCGGTC CGGAAACCTC
      361 TACGTATGCA AACGAGCAGA GCGTTAAGTT CTATGATGTG CTGACGCCGG ACCAACAGAA
      421 GGACCTGACC TCCAATGTCG AGAGCCTGCT GAGCCCGGCG AAGGCAATGG TGGATCTGCG
      481 TAACGCAATC TCTACGAGCA CCTATAGCGT CCTGATCGGT AGCTTCGGTT CCCGTTGGAT
      541 TGACGACATC AAATTTGATT ACGAAAATGC GGAAATGACC TACGGTAACA CCGGTGCTGA
      601 TGGCGAAGGT TTCATTAGCG GCATGAACTT GAACTTCTCG TCTACGTACC GTTACCGCGA
      661 CGCCGAACAG GCGACGGTTA CCAATACCGT TCGCGGCACC GTGGTCATTA CCGTCAGCGA
      721 TAATGGCGAA ATTATTGCAG CAATTAATAA GATCTCTAAT GAATTGGCGG GCGACATGCT
      781 GAAGGAGGCG AATAGCAAAG TCTATATCGA CTTCGAAACC CTGAAAGCGC TGGAGCCGTC
      841 GCTGACGATT CAAGATGTCT TGACGGCAAA CACCACCACG TATAAGAGCG CAATTAATAA
      901 ATACAACCAA TCCTTCGCTA AGTCCATGAC CTCGACTATT GAGAATAAGT ACTTCCGTAA
      961 TACTAGCTCT GCGGTGGTTA ACGCGATTAA TGTCAGCTAC TATAATGAGA ACGACATTAT
     1021 TCGTCAAGAA CAAACCAACC ACTTCAGCAA CACGGGTAAA ATTAGCGTGG AGTTCGACCA
     1081 AGGCGCCTGG CCTGAATGGA ACACCTTTTT CGGTAAGGTT AAGACCACCG ATCAGATCCA
     1141 GCTTGATGGT AGCCGCGTAA ATGGTGATCA GGTCCTGTAT AAAAGCCTGG AAAATCAGTG
     1201 GCAACAAGTT CTGCAAAACT ACAACAATAA ACTGAAATCC GAATACGACC GCCGTGTTCA
     1261 GACCAGCGTG ATTCAAGAAA ACGAACGTAC CCCTGACCTG TTGGCCATGA GCATCAGCTC
     1321 GGAAACGATC ACCAATAAGG GTCTGACCAT CAGCCTGCGC AACGGTTACA CCCAGCGCTT
     1381 ATCGGATGTC GGTTTCACCT ATAGCATTGC CATCGACAAT AAGGCTACGA GCGCGAGCGA
     1441 AGTGAACAGC CCGCAGACCT CCAAGATTTT CAGCGCTTAC TACAAAGGCA TTGAACAAAT
     1501 GCTGAATACC TTTCACAAGT TTTACGGTAT CTCCGAGAGC GACATCTGGA GCTCTCCGAA
     1561 CGCGAGCCTG CAGCAAAAAC AAAACAAGTT TAAGATGAGC GGCAAAACCG GCGTTAAGAA
     1621 AGAGGATGGC ACGGACTTTA ACATTTGGGA CTATTGGAAG GAAACCGGTG CCATGAATGG
     1681 CACCATCAAT GGTACCAATT TCGCAAACGC GTTGAGCCTG AATACGGGCG TTGAGGCCCA
     1741 GCAAATCAAA GAACAAAATT TGATCAGCAA AATTGAGGGC CTGACCTATC TGAATCTGAC
     1801 CTATTCTGAC GTTAACAGAA ACAGCAGCCA GAGCAATTAT AGAGGTGTCC GTAGCGCCAC
     1861 GATCGCGGAC TCCAGCTACA CGGGTAACAA TGGTACCATG CTGATGAGCG GTCTGAGCAT
     1921 GAGCACCCGT AACAACTCTG CACTGACCTT TGGTATTTCA ACCGATCTGC TGGATGTCTC
     1981 CTTCGGTAAG GGTCAGCGTT TCGATATCCA AAGCCACGGC CGTTACACCT TTATTGAGCG
     2041 TGTTCAGTGA AGAGCTTAGA GACCGCCACG GCGAAATTTA ATGTCGCTGA GGTGTCAATC
     2101 GTCGGAGCCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG
     2161 GGTTTTTTGC ATGGTCATAG CTGTTTCCTG AGAGCTTGGC AGGTGATGAC ACACATTAAC
     2221 AAATTTCGTG AGGAGTCTCC AGAAGAATGC CATTAATTTC CATAGGCTCC GCCCCCCTGA
     2281 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     2341 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     2401 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     2461 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     2521 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     2581 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     2641 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     2701 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     2761 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     2821 TACGCGCAGA AAAAAAGGAT CTCAAGAAGG CCTACTATTA GCAACAACGA TCCTTTGATC
     2881 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2941 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     3001 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     3061 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     3121 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAA
     3181 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     3241 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     3301 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     3361 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     3421 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     3481 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     3541 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3601 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3661 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG
     3721 CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA
     3781 CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA
     3841 AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC
     3901 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
     3961 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACCTGCAC CAGTCAGTAA
     4021 AACGACGGCC AGTAGTCAAA AGCCTCCGAC CGGAGGCTTT TGACTTGGTC AGGTGGAGTG
     4081 GGAG
//