LOCUS       Exported     3878 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3878
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3844
                     /note=""
                     /translation="MVEENYEKKKTNPLQNFIKQKLIKYRRIPFVKLMKFSFKSLLKEKLFYILNLATIIISILMGIILAFVKSGSSQVVIFNFYILFFVCCLMFVFILRMIQFFFSKNFEDKTTYIVLTNQVSRTKFFIAQYLLIILICAVNILMSFIVINIFYAVFTLFHYDVFILRMTSMYVIYCLLATFFLINFISFLIFIFTLQTTTIICTLLLALSFIANIPMSFIKANEKSYYVQFTSGDIFQLNDIYDAYSLYDHVNDGNIKYPHLSKYVYNYFLSKEMVTDQFRNTTNINYRTQMWKDLGLINPNPVVITETNLDLFSKPLRDTSVPNTWAIYDKFNIQITLKDTFITNDQLDELINKTVDQNTKNILVEFRNFTNEINKYFNNDLQFEKYDLFYDFLFLDSGIEKSYLEKLNPSEEEIEENKVTYALKKQDVVSFYEYSVAGIRNDGFRFTNANDLVRNQLNFNLMYSARVIEEYFIKYSSNYIIMSSNAVSKTSGDWNSYTKGRSMMRGLSYFNLYSGLWMVYTKNLGFYNNDIWFSPNSFSKIYLEDQKNLFLGYPEYDIKLTSTNMIEKNTTSNYIKPWYYLVILFGISTFSFSIALYKFRKFDF"
                     /label="Mfl460"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGACTGC ATACCAGGTT GGGTCTCAAA TGGTTGAGGA
     2041 AAATTACGAA AAGAAAAAGA CTAATCCGTT GCAGAACTTT ATTAAACAAA AGCTGATCAA
     2101 GTACCGTCGT ATCCCGTTTG TTAAGCTGAT GAAGTTCAGC TTCAAGAGCC TGCTGAAAGA
     2161 AAAACTGTTC TACATCTTAA ACCTGGCCAC CATCATTATT AGCATCCTGA TGGGTATCAT
     2221 CCTGGCGTTC GTCAAGAGCG GTAGCTCTCA AGTGGTGATC TTCAACTTTT ATATTCTGTT
     2281 CTTCGTGTGC TGCCTGATGT TCGTCTTTAT CCTCCGCATG ATTCAATTCT TCTTCAGCAA
     2341 AAACTTTGAA GATAAGACGA CGTACATTGT CCTGACGAAT CAGGTCAGCA GAACTAAGTT
     2401 TTTTATCGCA CAATATCTGC TGATCATCCT GATTTGTGCG GTTAACATCC TGATGAGCTT
     2461 CATTGTTATT AATATTTTTT ACGCGGTGTT TACCTTGTTT CATTACGACG TGTTTATCCT
     2521 GCGCATGACT TCTATGTACG TCATCTACTG CTTATTGGCA ACTTTCTTTC TGATTAATTT
     2581 TATCAGCTTC CTGATCTTCA TCTTCACGCT GCAAACGACG ACCATCATTT GTACTCTGTT
     2641 GTTGGCACTG AGCTTTATCG CAAACATTCC GATGTCTTTC ATTAAGGCCA ATGAAAAAAG
     2701 CTACTACGTT CAGTTTACTA GCGGTGATAT TTTCCAACTG AACGACATTT ACGATGCTTA
     2761 CAGCCTGTAC GACCATGTGA ATGACGGCAA TATTAAATAC CCACATCTGT CGAAATACGT
     2821 GTACAATTAT TTTCTGAGCA AAGAAATGGT TACCGACCAA TTTCGTAATA CCACGAATAT
     2881 CAATTATCGT ACTCAGATGT GGAAAGACCT GGGTTTGATC AATCCGAACC CGGTCGTTAT
     2941 CACGGAAACC AACCTGGACC TGTTCAGCAA ACCGCTGAGA GATACTAGCG TCCCGAATAC
     3001 GTGGGCGATT TATGATAAAT TCAACATCCA AATTACCCTG AAAGACACTT TTATCACGAA
     3061 TGATCAGTTG GATGAGCTGA TTAACAAAAC TGTTGATCAG AATACCAAGA ATATCCTGGT
     3121 GGAGTTCCGC AACTTTACGA ATGAGATCAA CAAATACTTC AATAATGATC TGCAATTCGA
     3181 GAAGTACGAT TTATTCTACG ACTTCCTGTT CCTGGATAGC GGTATTGAAA AGAGCTATCT
     3241 GGAAAAATTG AATCCGAGCG AAGAGGAGAT TGAAGAAAAT AAAGTGACCT ACGCACTGAA
     3301 GAAGCAAGAT GTGGTCAGCT TCTATGAGTA CAGCGTCGCA GGCATCCGTA ATGACGGCTT
     3361 CCGTTTCACC AACGCGAATG ATTTGGTTCG TAATCAACTG AACTTCAACC TGATGTATAG
     3421 CGCCAGAGTC ATCGAAGAGT ACTTCATTAA ATATTCCAGC AACTACATCA TCATGAGCAG
     3481 CAACGCTGTT AGCAAGACCA GCGGCGACTG GAATTCGTAT ACGAAGGGCC GTTCGATGAT
     3541 GCGCGGCTTA AGCTACTTTA ACCTGTACAG CGGTCTGTGG ATGGTCTATA CCAAAAATCT
     3601 GGGTTTTTAC AACAACGACA TTTGGTTCTC ACCGAACAGC TTCTCTAAGA TTTACCTGGA
     3661 GGACCAGAAA AACCTGTTTC TGGGCTATCC GGAATACGAC ATCAAACTGA CTTCGACGAA
     3721 TATGATTGAA AAGAACACCA CCAGCAATTA TATTAAGCCG TGGTACTACT TGGTTATTCT
     3781 GTTTGGTATC TCCACGTTTA GCTTTTCTAT CGCACTGTAC AAGTTCCGTA AATTTGACTT
     3841 TTGAAGAGCT TAGAGACCGT GTCGCTCGCA ATCCTAAT
//