LOCUS       Exported     3935 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3935
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
                     /label="T7 terminator"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             2030..3901
                     /label="Mfl461"
                     /note=""
                     /translation="MLKKVLPIASVFVLGFGLITTACGQKPKDFKQEYKPIYDYYVLGESFEQEVETKFHNALAKKQNEIIYIDQTMQQNKNLDFFTQSNLQEISLRKQNQNVSSNYELLSQREKDALNNDLNKITKNSKLEETILDSMKPEINFYSSILDFKNVDQKWFDGLSYDFNNIDFGYLNFDKENKNYIANVNLGLVFTYQYTDINKNIIKNNYHEDIVITISNQNDIIENLKFTTSELRENLIKNKFEWAWFDAYDLRVNDYARLFNLSNSDFENLFKFDKFEKSLLSYIYEHNTNLSKPTQSYNFAFKDENRFKHFKSIDKISNFDPRQNKYGSNELILDDTVSTTNTNNINLFKIIFSQLITDDQIVLENKFVKGNQDIYAYLSKEYKTWVSNFQSNVNEELNINSDSLVNFGEVTLNNFCLYFDEFNYYHPISPISYYVGISANNFNDSYLNGESEMVKNKIVLPDQDPIFKAIYQNTMSALQIFQESYGTSKTTWKSLSDNQQLISLKSSNILQSRAATTNDKSISDVNAQLSLLNNKSSTYSAESDRQSFLNKTNSSSFHFQFNNSEELQNIPIIEMNNNQLIFKKFKEGSSNLSVDFNLSFLNLSFVIKSEKFYWFERTLIESI"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGAACTA TGGCATCGAG CGGTCTCAAA TGCTCAAAAA
     2041 GGTCCTGCCG ATTGCAAGCG TCTTTGTGCT GGGTTTTGGT CTGATCACCA CTGCCTGTGG
     2101 CCAAAAACCG AAGGACTTTA AACAGGAATA CAAACCTATT TACGACTATT ACGTGCTGGG
     2161 TGAAAGCTTT GAGCAGGAAG TCGAAACCAA GTTCCACAAC GCACTGGCTA AGAAGCAGAA
     2221 TGAAATCATC TATATTGATC AGACCATGCA ACAAAATAAA AATCTCGACT TTTTCACCCA
     2281 AAGCAACTTG CAGGAAATCA GCCTGCGCAA GCAGAACCAG AACGTGAGCA GCAATTATGA
     2341 ACTGCTGTCA CAACGTGAGA AAGATGCGCT CAACAACGAT CTGAACAAGA TCACCAAGAA
     2401 CAGCAAATTG GAGGAAACGA TCCTTGACTC GATGAAACCG GAGATTAACT TTTATAGCTC
     2461 CATTCTGGAC TTCAAAAACG TTGATCAGAA ATGGTTCGAT GGCCTGAGCT ACGACTTTAA
     2521 TAACATTGAT TTCGGTTATC TGAACTTTGA TAAGGAGAAC AAGAATTACA TTGCGAACGT
     2581 TAACCTGGGT TTGGTTTTCA CCTATCAATA TACCGATATT AATAAAAACA TTATCAAAAA
     2641 CAATTATCAC GAGGATATCG TTATCACCAT TAGCAACCAA AACGATATTA TTGAAAATCT
     2701 GAAGTTTACC ACCAGCGAGC TGCGCGAAAA CCTGATCAAG AACAAATTCG AATGGGCGTG
     2761 GTTTGACGCG TACGATCTGC GTGTGAATGA TTATGCACGC CTGTTTAACC TGAGCAACAG
     2821 CGACTTCGAA AACCTGTTCA AGTTCGACAA GTTCGAAAAG AGCTTGCTGT CGTATATCTA
     2881 CGAACATAAT ACCAACCTGA GCAAGCCGAC CCAAAGCTAT AACTTTGCGT TTAAGGATGA
     2941 GAACCGTTTC AAGCATTTCA AGTCTATTGA CAAGATTAGC AACTTTGACC CGCGCCAAAA
     3001 CAAATACGGT AGCAACGAGC TGATCTTGGA CGATACCGTG AGCACCACTA ATACCAACAA
     3061 CATTAATCTG TTTAAGATCA TTTTCTCTCA GCTCATTACG GACGATCAAA TTGTTCTGGA
     3121 AAATAAGTTC GTTAAGGGCA ATCAAGACAT CTACGCCTAT CTGTCCAAAG AGTACAAAAC
     3181 CTGGGTAAGC AATTTCCAGA GCAATGTCAA TGAGGAGCTG AATATCAACA GCGATTCGTT
     3241 GGTCAATTTC GGCGAAGTGA CCCTGAACAA CTTTTGCCTG TACTTCGACG AATTTAATTA
     3301 CTACCATCCG ATTAGCCCTA TTAGCTATTA CGTCGGCATT AGCGCGAACA ACTTCAACGA
     3361 CAGCTATTTG AACGGTGAAA GCGAAATGGT TAAAAATAAA ATCGTGCTGC CGGACCAGGA
     3421 TCCGATTTTT AAGGCCATTT ATCAGAACAC CATGAGCGCG CTGCAGATTT TTCAAGAAAG
     3481 CTATGGTACC AGCAAAACGA CTTGGAAGTC CCTGAGCGAT AACCAGCAGT TAATTTCCCT
     3541 GAAGTCCTCT AACATTCTGC AGTCTCGCGC TGCGACCACC AACGATAAGA GCATTTCTGA
     3601 TGTGAACGCG CAATTGTCTC TGCTGAATAA CAAGAGCTCC ACGTACAGCG CGGAGTCAGA
     3661 TCGCCAATCT TTTCTGAACA AAACCAATTC TAGCTCCTTT CACTTCCAAT TCAATAATAG
     3721 CGAGGAGCTG CAAAACATCC CGATCATCGA GATGAACAAT AACCAGCTGA TCTTCAAGAA
     3781 GTTCAAAGAA GGTAGCAGCA ATCTGTCCGT GGACTTTAAC CTGAGCTTCC TGAATCTGTC
     3841 CTTTGTTATT AAGAGCGAAA AATTTTACTG GTTTGAACGT ACCTTGATCG AGAGCATTTG
     3901 AAGAGCTTAG AGACCGCCAT TGGAAAACTG AACGT
//