LOCUS       Exported     4931 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..4931
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..4897
                     /label="Mfl462"
                     /note=""
                     /translation="MEKNILKSKRVILENSNNNAVMVDHFHKKFKDINIGPFSFNIEKGKVTALLGSSGSGKSVFLNSLLGATLNYDGNIYINGKERKKSSSIENNSNVGFYSQMDFSLYSITAYDFLYNMCNVMGLDKTLVKSRIEYWLKKFDLWTSKDKPLNAFSWGMKNRMNLILCFIKEPEILILDEPGANLDSKWRGQSYKILKEFKEEFNSTIILTVHNIDEVYNIIENFVILEKGKLLFSGSKSELNLYKKINVSFEKDVVLAEVEKLLNQHNILTFNFDLSTNSLVIGLNQYQTFNDALAILEKNHIQPKDVVAQAINMDAITKALEDKEKPHKPKYEALKEFAIDSSNKSIDLDRALDFLNKIEDKYKNILNLKEDEIKVDLSSDLVLISENSPESSTESHEALVLNDPSYASEHVLTASEINMIKNDFFDKVQELKNLKLEINKIVTEQKNEIDLNIDDLKDYFAKIKLESEIIHQVKTSFEDEATNLKALLQQTEAETEELEFKKTDLQIKIDELKALQDEISSTSANEYEQINLVKTEFSNKLEDLKTYIEKVKIESENSLTSEIELKMNEFKEFENQFKDLVAVQREIIQEEIKDFKSYLTQIQIENDEINNSRDALDKDLSALKQSREEFLTEVDLEQEEVEMKKLELNIKLDELKKLQEDIAVATITEYEEIDSIKAEFTSKLIDLKSYVENISAETQIIQESKAEYLEKIKEFKEYHDEFNKTASAEKESIKNSISELKEFMSQIQIESSEVNQSKAELEEELKKLKETQSAILAKIEAENISTQSKQKQLESQYSEINTIQNNIATKVNLINEESTTIKESNFEILKLKEELEKLKYEMNEQQNMKRYVNSHQGLNNFSDNMQQPMWHQQNAISLKDQELEKIKSDLIKEKEYFEQLRKEMAFEKEMNQKLAKFDSEMKQRENVLELERIRREIQEEKNKLNEVLLMQKFNK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGACTGC ATACCAGGTT GGGTCTCAAA TGGAGAAGAA
     2041 TATCCTGAAA AGCAAACGCG TTATTTTAGA AAACAGCAAC AACAACGCAG TTATGGTCGA
     2101 CCATTTTCAT AAAAAATTCA AGGACATTAA CATTGGCCCG TTCAGCTTCA ATATTGAAAA
     2161 GGGCAAAGTT ACCGCGCTGC TGGGTTCTTC CGGTAGCGGC AAAAGCGTTT TCCTGAATAG
     2221 CTTGTTGGGC GCGACGCTGA ACTACGATGG TAATATCTAC ATCAATGGTA AAGAACGTAA
     2281 AAAGAGCAGC AGCATTGAAA ACAACTCCAA TGTGGGTTTC TACAGCCAGA TGGACTTTAG
     2341 CTTATATTCC ATTACGGCTT ATGATTTCCT GTACAACATG TGTAACGTTA TGGGCCTGGA
     2401 CAAGACCCTG GTGAAATCCC GTATCGAATA CTGGCTGAAA AAATTCGACC TGTGGACGAG
     2461 CAAAGACAAG CCGCTGAACG CGTTCAGCTG GGGCATGAAG AATCGTATGA ACCTCATTTT
     2521 GTGCTTTATC AAAGAGCCGG AAATTCTGAT CCTCGACGAG CCTGGCGCAA ACCTGGACAG
     2581 CAAGTGGCGT GGCCAGAGCT ATAAAATCTT GAAAGAATTT AAAGAGGAGT TCAACTCCAC
     2641 TATCATCCTG ACCGTCCATA ACATCGATGA AGTGTACAAT ATCATTGAGA ACTTTGTGAT
     2701 CTTAGAGAAA GGCAAACTGT TGTTCAGCGG TAGCAAAAGC GAGCTGAATC TGTACAAAAA
     2761 GATCAATGTC AGCTTCGAGA AGGACGTCGT GTTGGCGGAG GTCGAAAAGC TGCTGAACCA
     2821 GCACAATATC TTGACCTTCA ATTTTGATCT GTCGACGAAT TCCCTGGTTA TTGGCCTGAA
     2881 TCAGTATCAG ACGTTCAACG ACGCGCTGGC TATTCTGGAG AAAAACCATA TTCAACCGAA
     2941 GGACGTGGTG GCCCAGGCGA TCAACATGGA TGCAATCACC AAAGCGCTGG AGGACAAGGA
     3001 GAAACCACAC AAGCCGAAGT ACGAAGCCCT GAAAGAATTT GCAATCGACT CTAGCAATAA
     3061 ATCTATCGAC CTGGATCGTG CGCTGGACTT TCTGAACAAA ATTGAGGACA AATACAAGAA
     3121 TATTCTGAAT CTGAAAGAGG ACGAAATTAA GGTAGATCTG TCTAGCGATC TGGTCTTGAT
     3181 TAGCGAAAAC TCTCCGGAGT CCAGCACCGA ATCTCACGAA GCCCTGGTTT TGAACGATCC
     3241 GAGCTACGCA AGCGAACATG TGCTGACCGC GAGCGAGATC AATATGATCA AAAATGACTT
     3301 CTTTGACAAA GTACAGGAGC TGAAGAACCT GAAGTTAGAG ATCAACAAGA TCGTCACTGA
     3361 ACAGAAAAAC GAGATCGATC TGAACATTGA CGATCTGAAG GATTATTTTG CCAAAATCAA
     3421 ACTGGAATCC GAGATCATCC ACCAGGTTAA GACGAGCTTC GAGGACGAGG CGACGAACCT
     3481 GAAGGCGCTG CTGCAGCAAA CCGAAGCTGA AACGGAGGAG CTGGAGTTCA AAAAGACGGA
     3541 TCTGCAAATT AAGATTGACG AACTGAAGGC GCTGCAAGAC GAAATCAGCA GCACTAGCGC
     3601 AAACGAGTAC GAACAGATCA ACCTTGTTAA AACCGAATTC AGCAACAAGT TAGAGGACTT
     3661 AAAGACCTAT ATCGAGAAGG TTAAAATTGA ATCAGAAAAT AGCCTGACGA GCGAAATTGA
     3721 ACTGAAGATG AACGAGTTTA AGGAATTCGA GAACCAGTTT AAGGATTTAG TGGCAGTGCA
     3781 ACGCGAGATT ATCCAAGAGG AAATTAAGGA CTTTAAGAGC TACCTGACCC AGATCCAGAT
     3841 TGAGAACGAT GAGATTAACA ACAGCCGTGA TGCTCTGGAC AAGGATCTGT CCGCTTTGAA
     3901 ACAAAGCAGA GAGGAGTTTC TGACGGAAGT GGACCTGGAG CAAGAGGAGG TTGAGATGAA
     3961 AAAGCTGGAA CTGAACATTA AGCTGGACGA GCTGAAAAAA CTGCAGGAGG ATATCGCCGT
     4021 CGCCACCATT ACGGAATACG AGGAAATCGA TAGCATTAAG GCAGAGTTCA CGAGCAAACT
     4081 GATCGATCTC AAGAGCTACG TCGAAAACAT TAGCGCGGAA ACCCAGATCA TTCAGGAGTC
     4141 TAAGGCTGAA TACCTTGAGA AAATCAAAGA GTTCAAAGAA TATCACGATG AATTCAACAA
     4201 AACGGCAAGC GCTGAAAAAG AGAGCATTAA AAATTCTATC AGCGAGCTGA AGGAGTTCAT
     4261 GAGCCAAATC CAAATCGAAT CCTCCGAAGT TAATCAATCC AAAGCCGAGT TGGAAGAAGA
     4321 ATTGAAGAAG TTGAAAGAAA CGCAGAGCGC CATTTTGGCC AAGATTGAGG CGGAGAACAT
     4381 CAGCACCCAA AGCAAACAAA AACAGCTCGA GTCCCAGTAC AGCGAAATCA ATACGATCCA
     4441 GAATAATATC GCTACGAAGG TTAATCTGAT CAATGAAGAG AGCACCACCA TCAAAGAGAG
     4501 CAACTTTGAG ATCCTGAAGT TGAAAGAAGA ACTGGAAAAA CTGAAATACG AAATGAATGA
     4561 ACAGCAGAAC ATGAAAAGAT ACGTTAATAG CCATCAGGGT CTGAACAACT TCAGCGATAA
     4621 CATGCAACAG CCGATGTGGC ATCAACAGAA CGCGATTTCG CTGAAGGACC AGGAACTGGA
     4681 AAAGATCAAG AGCGATCTGA TCAAAGAAAA AGAGTACTTT GAACAGTTGC GTAAAGAAAT
     4741 GGCGTTTGAA AAAGAGATGA ATCAAAAACT GGCGAAATTC GATAGCGAGA TGAAGCAGCG
     4801 TGAGAACGTT CTGGAACTGG AGCGTATTCG TCGCGAGATC CAGGAGGAGA AAAATAAACT
     4861 GAATGAGGTG CTGCTGATGC AGAAATTCAA TAAGTGAAGA GCTTAGAGAC CGCTGGGCAC
     4921 TATGCTTTAC T
//