LOCUS       Exported     3902 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3902
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
                     /label="T7 terminator"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3868
                     /label="Mfl469"
                     /note=""
                     /translation="MSNQKIINIAVIAHVDAGKSTLVDALLKQGGAFRDNQEVVEQIMDSNDQERERGITIYSKNCAIEYKGTKINIVDTPGHADFSSEVERIMKTVDTVILLVDSSEGPMPQTRFVLSKALELGLNPILMINKIDKKDQRAEEVVEEVLELFMELDATDEQLEFKTLYGIAREGIAQLNLSDQGVDLSPMFDTIIEQVGTYPIELAEKPLKMQVSSLAYDSFIGRLGIGRIFEGKIAEGQTVSVVKNNGEVKQAKISKLTVYQGLNKVAVKEAFAGDIITFAGIEHISIGDTINELNNINPMEPITIEEPTMSMNFLVNTSPFAGKVGKFVTSRNIKERLEKELEVNVGLKVEPLDNPTIEGFKVLGRGELHLSVLIEQMRREGFELAISKPEVIFRKGDNGTLLEPMEKVILNIPTEYSGTVINKLNQRKGLMTDMDSDGVRDKIVYNIPLRALIGFRSEFTNDTHGEGIMVRSSNGFEPYKGEIESRKNGVLISMASGKTLPYALNNLEERGILFVGPQVEVYDGMIVGQHSRDNDLEVNPTTGKKLTNTRASGSDDSVKLTPPKLMTLEEALEYIEWDELVEVTPDDIRLRKRWLSNTERRQHRNDNKKVFD"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGACTGC ATACCAGGTT GGGTCTCAAA TGTCCAATCA
     2041 GAAGATTATT AATATTGCGG TGATCGCACA CGTCGACGCA GGCAAGAGCA CCCTGGTCGA
     2101 TGCACTGTTG AAACAAGGTG GTGCCTTTCG TGATAATCAA GAGGTCGTGG AACAGATTAT
     2161 GGATAGCAAT GACCAGGAGC GCGAACGTGG TATCACCATC TATAGCAAGA ATTGCGCCAT
     2221 CGAGTACAAG GGTACTAAAA TCAATATCGT GGATACGCCG GGTCACGCAG ACTTTAGCAG
     2281 CGAAGTTGAA CGTATTATGA AAACCGTCGA CACTGTCATC CTGCTGGTTG ACAGCAGCGA
     2341 AGGTCCGATG CCACAGACCC GCTTTGTTCT GAGCAAGGCG CTGGAACTGG GTCTGAACCC
     2401 AATTCTGATG ATCAACAAGA TCGATAAGAA AGACCAAAGA GCGGAGGAGG TTGTTGAGGA
     2461 AGTTCTGGAG CTGTTCATGG AGCTGGACGC AACCGACGAA CAACTGGAAT TCAAGACGCT
     2521 GTACGGTATC GCGCGCGAGG GTATTGCCCA GCTGAACCTG TCCGATCAGG GCGTCGATCT
     2581 GAGCCCGATG TTCGACACCA TTATCGAACA GGTTGGTACC TACCCGATCG AATTAGCGGA
     2641 GAAGCCGTTG AAGATGCAAG TTAGCTCTCT GGCGTATGAT TCCTTTATCG GCCGCCTGGG
     2701 CATTGGTCGC ATCTTCGAGG GCAAGATTGC AGAGGGTCAA ACTGTTAGCG TGGTGAAAAA
     2761 TAATGGTGAA GTCAAACAGG CCAAAATCAG CAAACTGACC GTTTACCAGG GTTTAAATAA
     2821 GGTGGCGGTC AAAGAGGCGT TCGCGGGTGA TATTATCACC TTTGCGGGCA TTGAACACAT
     2881 CAGCATCGGC GACACCATTA ATGAACTGAA CAACATCAAC CCGATGGAGC CGATCACCAT
     2941 CGAAGAACCG ACCATGTCTA TGAATTTCCT GGTGAACACC AGCCCGTTTG CCGGCAAAGT
     3001 CGGCAAGTTT GTGACGAGCC GTAATATTAA AGAACGTCTG GAGAAGGAGT TGGAAGTCAA
     3061 TGTTGGTTTG AAAGTGGAAC CGCTGGACAA TCCGACGATC GAAGGCTTCA AAGTGCTTGG
     3121 CCGCGGCGAA CTGCATCTGT CTGTATTAAT TGAACAAATG CGTCGTGAAG GCTTCGAACT
     3181 GGCGATTAGC AAACCGGAAG TGATTTTTCG TAAGGGTGAC AATGGTACCC TGCTGGAGCC
     3241 GATGGAAAAG GTTATTCTGA ACATCCCGAC CGAATACAGC GGTACGGTTA TTAATAAGCT
     3301 GAACCAGCGT AAAGGTCTGA TGACCGACAT GGATAGCGAC GGCGTGCGCG ATAAAATTGT
     3361 TTACAACATC CCGTTGCGTG CCTTGATTGG CTTCCGTTCT GAGTTTACCA ATGACACGCA
     3421 TGGCGAGGGC ATTATGGTCC GCAGCAGCAA CGGCTTCGAG CCGTACAAAG GTGAGATCGA
     3481 GTCCCGTAAG AACGGTGTTC TGATCAGCAT GGCGTCGGGT AAGACTTTGC CGTATGCACT
     3541 GAATAACTTG GAAGAACGCG GTATTCTGTT CGTCGGCCCG CAAGTTGAGG TCTATGACGG
     3601 TATGATCGTT GGTCAACACA GCCGTGATAA TGACCTGGAG GTTAATCCGA CGACCGGTAA
     3661 AAAACTGACG AATACCCGTG CGTCTGGCTC TGATGATAGC GTGAAGCTGA CCCCGCCGAA
     3721 ACTGATGACC CTGGAGGAAG CGTTGGAGTA TATCGAATGG GACGAATTGG TGGAGGTCAC
     3781 GCCTGACGAC ATTCGTTTGC GTAAACGCTG GCTGAGCAAC ACGGAGCGTC GCCAGCACCG
     3841 CAATGATAAC AAAAAAGTTT TCGATTGAAG AGCTTAGAGA CCGGCTCGAT ACAGTTTCAC
     3901 GT
//