LOCUS Exported 2872 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2872 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 910..957 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2805..2821 /note="common sequencing primer, one of multiple similar variants" /label="M13 for" terminator 2822..2853 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..838 /label="Mfl472" /note="" /translation="MTKFKYEDWLMQFINDDWYIQINTSENNIIFDEVIQLHEKWLDSQDYNNFIKENQEAVAIDNLPGFLENEEVCKTDEYIKSFISGVFHLRIDGLYNIASDYVNAFNEINEHSFNAVDESGVDVAINKAFLELSEKYYEELITVVRNTEVPDEFKYCWRDLIELVQRFNSYESREDKLDVAYQLLDYLTTTIDGFDDLSIDLTDEMIESSNNFIALLIKFEIIFDRLILLKEHIEYQYVEQKGLPDNFYRMNILDRYKEIETFKIMNEED" primer_bind complement(962..978) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1045..1633) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1824..2684) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2685..2789) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GACCAAATTC AAATACGAAG ATTGGCTGAT 61 GCAATTTATT AATGACGACT GGTATATTCA GATCAATACG TCCGAAAATA ATATTATCTT 121 CGACGAAGTG ATTCAGCTGC ACGAAAAGTG GCTGGATAGC CAGGACTACA ACAATTTTAT 181 CAAGGAGAAT CAAGAGGCCG TTGCGATCGA TAATCTGCCG GGCTTTCTTG AGAACGAGGA 241 GGTCTGTAAG ACCGATGAGT ACATCAAGTC CTTTATTAGC GGTGTTTTTC ACTTGCGTAT 301 CGACGGCCTG TACAATATCG CCAGCGACTA CGTTAATGCT TTTAACGAAA TCAACGAACA 361 CAGCTTTAAC GCAGTGGACG AGAGCGGTGT TGATGTGGCC ATCAATAAAG CCTTTCTGGA 421 GCTGAGCGAA AAATATTACG AGGAACTGAT TACCGTTGTT CGTAACACCG AGGTTCCGGA 481 TGAATTCAAA TATTGTTGGC GTGACCTGAT TGAGCTCGTC CAACGTTTTA ATAGCTACGA 541 AAGCCGCGAG GACAAACTGG ACGTTGCGTA CCAGCTGCTG GATTATCTGA CTACCACGAT 601 CGACGGTTTC GATGATCTGA GCATTGACCT GACTGATGAG ATGATCGAAA GCAGCAACAA 661 TTTTATCGCT CTGCTGATTA AATTCGAAAT CATTTTCGAC CGTCTGATTC TGTTGAAGGA 721 GCATATCGAG TATCAGTACG TTGAGCAGAA AGGTCTGCCG GATAACTTTT ACCGTATGAA 781 CATTCTGGAC CGCTACAAAG AAATTGAAAC GTTCAAGATT ATGAACGAGG AGGATTGAAG 841 AGCTTAGAGA CCCGTGAATC TTGACCTGTG GTCGCTGAGG TGTCAATCGT CGGAGCCGCT 901 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT 961 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG 1021 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 1081 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 1141 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 1201 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 1261 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 1321 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 1381 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 1441 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 1501 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 1561 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 1621 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG 1681 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 1741 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA 1801 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG 1861 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA 1921 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG 1981 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT 2041 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT 2101 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC 2161 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA 2221 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT 2281 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC 2341 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA 2401 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA 2461 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA 2521 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT 2581 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC 2641 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA 2701 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 2761 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG 2821 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG //