LOCUS       Exported     3866 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3866
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3832
                     /label="Mfl473"
                     /note="Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner."
                     /translation="MDKSKIRNFSIIAHIDHGKSTLADRILELTNTVTKREMQEQLLDSMDIERERGITIKLNSVQLYYKAKDGQEYTFHLIDTPGHVDFAYEVSRSLAACEGAILVVDATQGIEAQTLANVYLAIENNLEIIPVINKVDLPSADAERVKEEIENTIGIDCSDAPLISAKTGLNVEDVLEAIVNKIPAPYDADDAKPLRALIFDSYYDKYLGVVMSIRVREGSIKVGDRIKLMANGSSYEVTELGVKNPKIVKKDQLSAGEVGWVAASIKTIKDINVGDTITTVTNSALHPLDGYKKLKPMVYCGIYPIDTNQYQDFKEALEKMELSDSSLVYEPETSQALGFGFRVGFLGLLHMEVVQERLEREYNLNLIATAPSVIYKIHLTDGTMIEIDNPAKLPDPQKIKFMEEPFVNVKIMTPKESVGDLMSLCQNKLGTYKDLQVVDDNRMMLVYDMPLAEIIFDFFNKLKSISKGYASFEYEMIGYQESQLVKMDILLNGDMVDAFSMIVNKHFAYQRGAALTKKLKELIPRQNFEVPVQATIGNKVLARETIKAYRKDVTWKLHAADKSRRKKLLNKQKEGKKKMKEIGSVEVPQEAFIAVLKLDD"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAAGCACTC TTAGGCCTCT GGGTCTCAAA TGGACAAAAG
     2041 CAAGATTCGT AACTTTAGCA TTATTGCACA CATTGATCAT GGTAAAAGCA CCCTGGCGGA
     2101 CCGCATTTTG GAGCTGACCA ACACGGTGAC CAAGCGTGAG ATGCAAGAAC AACTGTTAGA
     2161 CTCCATGGAT ATCGAGCGTG AACGTGGTAT TACCATTAAG TTGAATTCGG TTCAGCTCTA
     2221 CTATAAGGCT AAGGATGGTC AGGAGTATAC CTTCCACCTG ATCGACACTC CGGGCCATGT
     2281 TGATTTCGCG TACGAAGTGT CTCGTTCCCT GGCCGCTTGT GAAGGTGCAA TCCTGGTAGT
     2341 GGATGCTACC CAGGGCATCG AGGCCCAAAC TTTGGCAAAC GTGTACCTGG CCATTGAGAA
     2401 TAACCTGGAG ATCATCCCGG TGATTAACAA GGTCGATCTG CCAAGCGCGG ATGCGGAACG
     2461 TGTGAAAGAA GAAATTGAAA ACACCATCGG TATCGACTGT AGCGACGCGC CTTTGATTTC
     2521 CGCGAAAACG GGCCTTAATG TTGAGGATGT TCTGGAGGCG ATTGTTAACA AGATCCCGGC
     2581 GCCGTATGAC GCGGACGACG CTAAGCCGCT GCGCGCGTTG ATCTTCGACA GCTACTATGA
     2641 CAAGTACCTG GGCGTCGTGA TGAGCATCCG TGTCCGTGAG GGTAGCATCA AGGTTGGTGA
     2701 TCGCATCAAG CTGATGGCGA ACGGTTCTTC TTACGAGGTT ACCGAGCTGG GCGTTAAGAA
     2761 TCCGAAGATC GTTAAAAAGG ATCAGCTCAG CGCTGGTGAA GTCGGTTGGG TTGCGGCGAG
     2821 CATCAAAACC ATCAAAGATA TTAACGTTGG CGATACCATT ACGACGGTGA CCAACAGCGC
     2881 ACTGCACCCG CTGGACGGTT ACAAGAAGCT GAAACCGATG GTGTATTGCG GCATCTACCC
     2941 GATCGACACG AACCAATATC AGGATTTTAA GGAAGCACTC GAAAAAATGG AACTGTCCGA
     3001 TAGCTCCCTG GTTTATGAAC CGGAAACTAG CCAAGCACTG GGTTTTGGTT TTCGCGTAGG
     3061 TTTCTTGGGC CTGCTGCATA TGGAGGTCGT GCAGGAACGT CTGGAGCGTG AGTATAACTT
     3121 GAACCTGATT GCCACCGCTC CGAGCGTGAT TTACAAGATT CACTTGACGG ATGGTACGAT
     3181 GATTGAGATC GACAACCCGG CTAAACTGCC GGACCCGCAG AAAATTAAAT TCATGGAGGA
     3241 GCCTTTCGTT AATGTCAAAA TTATGACCCC GAAGGAGTCT GTCGGTGATC TGATGAGCCT
     3301 GTGTCAAAAT AAACTGGGCA CCTATAAAGA TCTGCAAGTC GTAGATGATA ACCGTATGAT
     3361 GCTGGTTTAC GACATGCCTC TGGCGGAAAT CATCTTCGAT TTCTTCAACA AACTCAAGAG
     3421 CATTAGCAAA GGTTATGCAA GCTTCGAGTA CGAAATGATC GGTTATCAAG AAAGCCAGCT
     3481 GGTGAAGATG GACATTTTAT TAAATGGCGA CATGGTTGAC GCCTTCAGCA TGATTGTCAA
     3541 CAAGCACTTC GCGTACCAGC GCGGTGCAGC TCTGACGAAG AAATTGAAAG AACTGATCCC
     3601 GAGACAGAAC TTCGAGGTTC CAGTGCAAGC AACCATCGGT AATAAAGTCC TGGCGCGTGA
     3661 AACCATCAAG GCTTACCGTA AGGACGTGAC CTGGAAGCTG CATGCGGCGG ACAAGAGCCG
     3721 TCGCAAGAAG TTGCTGAACA AACAAAAGGA GGGCAAAAAG AAGATGAAAG AGATCGGTAG
     3781 CGTGGAAGTT CCGCAAGAAG CGTTTATCGC GGTTCTGAAA TTGGACGATT GAAGAGCTTA
     3841 GAGACCGTGT CGCTCGCAAT CCTAAT
//