LOCUS       Exported     2992 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2992
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1030..1077
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2925..2941
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2942..2973
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..958
                     /label="Mfl474"
                     /note=""
                     /translation="MRKFQLQMIDGKELINFEWKTSKKPIAIIQVVPNFDEHMEMYDKFAKIMKEHNILVVGTDLRGIGESRDESDGSNIIFDKKQGWSKLVEDVKNINTWIKRYHSDLPIFVMGQGLGGNLARAFSIKYSEEMAGLILINTRDYNYHISNIFLKYMNLSQMIFNVRNDAKFLNNIRAKRMNKRHNPLLKFDNQWLSSDWKFVEKYNNDPLCNLKLSWSAFKDIAVGNKFISKNSNNEFITKDLPILIQSGGLDNYTKMGKDSQKLFYRFTKLGLDTDLKIYQNLKNKLLEEEVNEVVIDDIVKFIDKYKDNY"
     primer_bind     complement(1082..1098)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1165..1753)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1944..2804)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(2805..2909)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAGAAAGTTT CAACTGCAAA TGATTGACGG
       61 TAAGGAGTTG ATTAACTTCG AGTGGAAAAC TAGCAAGAAG CCGATTGCAA TCATTCAGGT
      121 GGTTCCGAAT TTCGACGAGC ACATGGAAAT GTATGACAAA TTCGCGAAGA TCATGAAAGA
      181 ACACAACATT TTGGTGGTTG GCACCGATCT GCGCGGTATC GGCGAAAGCC GTGATGAATC
      241 GGATGGCTCC AATATTATTT TCGATAAAAA GCAAGGTTGG TCTAAATTAG TGGAGGACGT
      301 GAAAAATATT AACACGTGGA TCAAGCGCTA CCACAGCGAT CTGCCGATTT TCGTGATGGG
      361 TCAGGGTTTG GGCGGTAATC TGGCGCGCGC ATTCAGCATT AAATACTCTG AAGAAATGGC
      421 GGGCCTGATT TTGATCAACA CTCGTGACTA CAACTACCAC ATCAGCAACA TCTTCCTGAA
      481 GTACATGAAC CTGAGCCAAA TGATCTTTAA CGTTCGTAAC GACGCAAAAT TTCTGAACAA
      541 CATCCGTGCC AAGCGTATGA ACAAACGTCA CAATCCGTTG CTGAAATTCG ACAACCAGTG
      601 GCTGAGCTCC GACTGGAAGT TTGTGGAAAA GTACAACAAC GACCCGCTGT GCAATCTGAA
      661 GTTGAGCTGG AGCGCATTCA AGGATATTGC GGTCGGTAAT AAGTTTATTA GCAAGAATAG
      721 CAACAACGAG TTTATTACCA AAGATCTGCC GATTCTGATT CAGAGCGGTG GCCTGGACAA
      781 TTACACTAAA ATGGGTAAAG ATAGCCAGAA GCTGTTCTAC CGCTTCACCA AGCTGGGCCT
      841 GGACACCGAT TTGAAAATCT ACCAGAATCT GAAGAATAAA CTGCTGGAGG AGGAGGTCAA
      901 CGAGGTGGTC ATCGACGACA TTGTTAAATT TATTGATAAG TATAAAGACA ACTACTGAAG
      961 AGCTTAGAGA CCGGCCTTTT ATTGGGTCCA GTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1021 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1081 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1141 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     1201 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     1261 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     1321 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     1381 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     1441 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     1501 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     1561 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     1621 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     1681 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     1741 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     1801 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     1861 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     1921 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     1981 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2041 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2101 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     2161 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     2221 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     2281 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     2341 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     2401 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     2461 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     2521 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     2581 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     2641 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     2701 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     2761 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     2821 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     2881 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     2941 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//