LOCUS       Exported     2683 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..2683
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      721..768
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2616..2632
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2633..2664
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..649
                     /note=""
                     /translation="MKYYAVKQGRNIGVYETWEECKIQVEGYTNAVYKSFSSKADAEAFIKGGQTLSKPKQKIEIEENAAVAYSDGSFIKDNNTYSYGAVVMWQNKEFHFSKRYRDDELKSMWNVSGELQGAKRVMLFAYANNIKKLYLYHDYEGIAKWANHEWKAKSDEGNEYIKFVDQIRTKVELEFIWVKGHSNDYYNDLADQLAANATFEEYVKEV"
                     /label="Mfl475"
     primer_bind     complement(773..789)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(856..1444)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1635..2495)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2496..2600)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AACGATGATG CTCACTCTCG GGTCTCAAAT GAAATACTAT GCGGTCAAGC AGGGTCGTAA
       61 CATTGGTGTC TATGAAACCT GGGAGGAATG TAAGATCCAA GTGGAGGGCT ACACGAATGC
      121 GGTTTACAAA AGCTTCTCGA GCAAAGCAGA CGCTGAGGCG TTCATTAAAG GTGGTCAGAC
      181 CCTGTCGAAA CCGAAGCAAA AGATTGAGAT TGAAGAAAAC GCGGCGGTTG CGTACTCAGA
      241 TGGTAGCTTC ATCAAGGACA ACAACACCTA CAGCTATGGC GCGGTCGTCA TGTGGCAAAA
      301 CAAGGAATTT CATTTCAGCA AGCGTTACCG CGACGACGAG CTGAAGTCTA TGTGGAATGT
      361 TAGCGGTGAG CTGCAGGGTG CGAAGCGTGT CATGCTGTTC GCGTATGCGA ACAATATCAA
      421 GAAGCTGTAC CTGTACCATG ATTACGAGGG TATTGCGAAG TGGGCCAACC ACGAGTGGAA
      481 AGCGAAATCC GATGAAGGCA ATGAATACAT CAAATTCGTG GATCAAATCC GTACCAAAGT
      541 TGAGCTGGAA TTTATTTGGG TTAAAGGTCA CAGCAACGAT TACTACAATG ATCTGGCTGA
      601 CCAGCTGGCG GCCAACGCAA CCTTTGAGGA ATATGTTAAA GAAGTGTGAA GAGCTTAGAG
      661 ACCGCAGCGA TTCGGATACT ACTCGCTGAG GTGTCAATCG TCGGAGCCGC TGAGCAATAA
      721 CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCA TGGTCATAGC
      781 TGTTTCCTGA GAGCTTGGCA GGTGATGACA CACATTAACA AATTTCGTGA GGAGTCTCCA
      841 GAAGAATGCC ATTAATTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG
      901 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG
      961 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT
     1021 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT
     1081 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG
     1141 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT
     1201 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT
     1261 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT
     1321 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC
     1381 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC
     1441 TCAAGAAGGC CTACTATTAG CAACAACGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
     1501 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
     1561 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
     1621 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
     1681 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
     1741 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGAAC CACGCTCACC GGCTCCAGAT
     1801 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
     1861 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
     1921 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
     1981 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
     2041 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
     2101 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
     2161 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
     2221 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
     2281 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
     2341 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
     2401 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
     2461 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
     2521 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
     2581 AAACAAATAG GGGTTCCGCG CACCTGCACC AGTCAGTAAA ACGACGGCCA GTAGTCAAAA
     2641 GCCTCCGACC GGAGGCTTTT GACTTGGTCA GGTGGAGTGG GAG
//