LOCUS       Exported     3485 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3485
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 for"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3451
                     /label="Mfl476"
                     /note="Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro)."
                     /translation="MKQLEKITPRDVDFSQWYTDTVINAKLASYGPVKGTIIFRPYGYAIWELIQKYLDAKFKELEVQNVYFPLLIPQSLFQKEKDHIEGFSPEIATVTRVGDTPLPEPLFIRPTSEVLMANFFKNEVKSYRDLPLIFNQWTNVMRWEKTTRPFLRTSEFLWQEGHTVHSERKEASDLTLKILDTYTEFAQNALLLPVIPGKKTEKEKFAGADSTYTIESLMHDGQALQCGTSHYFADNFSKPYEIKFQNKEGKLEHAYSTSWGVSTRLIGAIIMTHSDDNGLVLPSMVSPVQVRLIQIKETDEVIKVTEDIKEALKNKYRVDIDKTDKSFGFKISEAEIKGIPLRIEIGPRDLENNQVTISRRDTREKIQVNVNEVTNIVDQMIKEYDANLYAKALKNREERTSRANSIEEYKNILAQNQGFVLVPFCGEIECEDDVKKQTSTNSRCIPFDQDNKTEKCFNCNKDTTLKVYFARAY"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAATGTCCTT AGGCAGTCGT CGGTCTCAAA TGAAGCAACT
     2041 GGAGAAGATC ACTCCTCGTG ACGTGGACTT CAGCCAGTGG TACACGGACA CCGTCATTAA
     2101 CGCGAAGCTG GCGTCGTATG GTCCGGTCAA AGGTACGATT ATTTTTCGTC CTTATGGTTA
     2161 TGCGATCTGG GAGCTGATTC AAAAATACCT GGACGCAAAG TTCAAAGAAC TGGAAGTGCA
     2221 AAATGTTTAC TTTCCGCTGT TGATTCCGCA GAGCCTGTTC CAGAAAGAGA AGGACCACAT
     2281 TGAGGGTTTT AGCCCTGAGA TCGCGACCGT CACCCGTGTT GGTGATACCC CGCTGCCGGA
     2341 GCCGTTGTTC ATCCGCCCGA CCAGCGAGGT ACTCATGGCA AACTTTTTTA AAAATGAGGT
     2401 TAAAAGCTAC CGTGATCTGC CGCTGATCTT TAACCAATGG ACGAACGTTA TGCGTTGGGA
     2461 GAAAACCACT CGTCCATTCC TGCGTACGTC TGAGTTCTTG TGGCAAGAAG GTCATACCGT
     2521 TCATAGCGAA CGTAAGGAGG CGAGCGACCT GACGTTAAAG ATCCTGGACA CTTACACGGA
     2581 ATTTGCACAG AACGCACTGC TGCTGCCGGT TATTCCGGGC AAAAAGACCG AAAAGGAGAA
     2641 GTTTGCGGGC GCTGATAGCA CCTACACCAT CGAAAGCTTG ATGCATGACG GCCAAGCGCT
     2701 GCAGTGTGGT ACCTCCCATT ACTTCGCGGA CAATTTTTCG AAGCCGTACG AGATTAAGTT
     2761 CCAGAACAAG GAGGGTAAGC TGGAGCACGC GTACAGCACG AGCTGGGGCG TTAGCACCCG
     2821 CTTGATCGGT GCCATCATTA TGACGCATAG CGACGACAAC GGCTTGGTGC TGCCGAGCAT
     2881 GGTGAGCCCG GTCCAAGTCC GTCTGATCCA AATCAAGGAA ACGGATGAGG TGATTAAAGT
     2941 CACGGAGGAC ATTAAGGAAG CCCTGAAGAA CAAATACCGT GTTGATATCG ATAAAACGGA
     3001 TAAATCCTTT GGCTTTAAGA TTAGCGAAGC GGAGATCAAG GGTATTCCGC TCCGTATCGA
     3061 GATCGGTCCG CGTGACCTGG AAAACAACCA GGTGACCATC AGCAGACGCG ATACCCGTGA
     3121 GAAAATCCAG GTTAACGTCA ACGAGGTCAC CAACATCGTG GATCAGATGA TTAAAGAATA
     3181 TGACGCCAAC TTGTACGCCA AAGCCCTGAA AAACCGTGAG GAGCGTACGT CCCGTGCGAA
     3241 TAGCATTGAG GAATACAAAA ACATCCTGGC TCAGAATCAA GGCTTTGTCC TGGTGCCATT
     3301 CTGCGGCGAG ATTGAGTGTG AGGACGATGT TAAAAAACAG ACCAGCACGA ACAGCCGCTG
     3361 CATTCCGTTC GACCAAGATA ACAAGACCGA GAAATGTTTT AATTGCAACA AAGACACCAC
     3421 CTTGAAGGTG TATTTTGCTC GTGCGTACTG AAGAGCTTAG AGACCCCCCA CTGTTAGATT
     3481 GAGCT
//