LOCUS Exported 3416 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3416 /note="transcription terminator for bacteriophage T7 RNA polymerase" /label="T7 terminator" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3382 /label="Mfl479" /note="Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY." /translation="MAFGDFLTKRMKKSMEKNIKKSTLTDENIQETLREIRLALLEADVNVEVVKELISKIKEKTVGEYIQEGVTSHQQMLKIVHEELIDILGTEKAPLNLSKKPSVVMMVGLQGSGKTTSSSKLAHYLAKKEAKKPLLVGLDIYRPGAIDQLVELGNKNNIPVFEQGKQSPLKTAKQALEFAEKNGHDVVILDTAGRLQIDKDLMNELNDLRKITSPNETLLVVDGMIGQEIINVTNEFNNQLKLTGVIVTKLDGDARGGATLSIRYMTKLPIKFIGEGEGVSALAPFYPKRMADRILGMGDIETLFEKVVDNIDERSMQKTMKRMFMGQFDLEDLRNQLVQVSKMGNIGGIMKMMPNAKISDNQISDAQRKLVVFSILMDSMTLKERREPKLLKSLTRKNRIISGSGRTEKELNELINSFEKGKKQVLEMAKMMKSGRMPGMPGMGKGGFGF" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" promoter complement(1813..1917) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAATCAGTTT CTTTGGCAGC CGGTCTCAAA TGGCGTTCGG 2041 TGATTTCCTG ACCAAACGCA TGAAGAAAAG CATGGAGAAA AACATTAAAA AGTCGACGCT 2101 GACCGACGAA AATATTCAGG AAACCCTGCG CGAAATTCGT CTGGCATTGC TGGAAGCAGA 2161 TGTGAATGTT GAAGTGGTGA AAGAACTGAT TAGCAAAATT AAAGAAAAGA CCGTTGGCGA 2221 ATACATCCAG GAGGGTGTTA CCAGCCACCA ACAAATGCTG AAAATCGTCC ATGAGGAACT 2281 GATCGATATT CTGGGTACCG AGAAGGCGCC GCTGAACCTG TCGAAGAAAC CGAGCGTTGT 2341 CATGATGGTT GGCCTGCAAG GTAGCGGCAA AACGACGAGC AGCTCTAAAC TGGCGCACTA 2401 CCTGGCCAAA AAGGAAGCGA AGAAGCCGCT GTTGGTGGGC CTGGACATCT ATCGTCCTGG 2461 TGCGATTGAT CAGCTGGTCG AACTGGGTAA TAAAAATAAT ATTCCGGTGT TTGAGCAAGG 2521 TAAACAATCC CCGCTGAAAA CCGCGAAGCA AGCCCTGGAG TTCGCCGAGA AAAATGGCCA 2581 TGACGTTGTC ATCTTGGACA CGGCTGGCCG TCTGCAGATC GATAAAGATC TGATGAACGA 2641 GTTGAATGAT CTGCGTAAAA TTACGAGCCC GAATGAAACG TTGTTGGTGG TCGATGGTAT 2701 GATCGGTCAG GAAATTATCA ACGTTACCAA TGAATTCAAT AATCAGCTGA AACTGACCGG 2761 CGTGATCGTC ACGAAACTGG ACGGTGATGC ACGTGGCGGT GCGACGCTGA GCATCCGTTA 2821 CATGACTAAG CTGCCGATTA AATTTATTGG CGAAGGTGAG GGTGTGTCTG CACTGGCTCC 2881 GTTTTACCCA AAGCGTATGG CGGATCGTAT TCTGGGTATG GGCGATATCG AAACGCTGTT 2941 CGAAAAGGTC GTGGATAACA TCGACGAGCG CAGCATGCAA AAAACCATGA AACGCATGTT 3001 TATGGGCCAG TTCGATCTTG AGGACTTGCG CAACCAATTA GTGCAAGTTT CTAAAATGGG 3061 CAACATCGGT GGTATCATGA AGATGATGCC GAATGCTAAG ATTAGCGACA ATCAGATCTC 3121 CGATGCGCAA CGCAAACTGG TGGTTTTTAG CATCCTGATG GATAGCATGA CGCTGAAAGA 3181 GCGTCGCGAA CCGAAACTGC TGAAAAGCCT GACCCGTAAG AACCGTATTA TCAGCGGTTC 3241 CGGTCGTACC GAGAAAGAAT TGAACGAGCT GATTAATTCC TTCGAGAAGG GTAAGAAACA 3301 GGTCCTGGAA ATGGCAAAAA TGATGAAAAG CGGTCGTATG CCGGGCATGC CGGGTATGGG 3361 TAAGGGTGGT TTCGGTTTTT GAAGAGCTTA GAGACCCCAG CCTCAGATTG TTGTCT //