LOCUS Exported 3428 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3428 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /note="bidirectional E. coli tonB-P14 transcription terminator" /label="tonB terminator" CDS 2030..3394 /label="asnC" /note="" /translation="MDIKFLYDNHETLKEQKVTIIGRVRSNRQGKAVSFMVLNDGTVLNDLQVVYKPETFGFEQGSKARVSSIVEIEGILIPTPTRPQPFEINATSITLLDQAIEEYPLQKKEHSPEFLREISHLRARTKTFQAIFRIRSTAAFAIHKFFQENNYVYVTTPIITSNDAEGAGEQFVVTVNEDKDYANDFFGKKASLTVSGQLNGEAFAQAFKNIYTFGPTFRAENSHTTKHASEFWMIEPEVAFADINDNIQLMQDMLKSVVGYVLEKNMTELEFLQEQLEPGLIDKITGIVNVEFKVNTYEEVLKTLQDAIDKGHKFEENNIHFGLDLGTEHERFICEEVNKCPTFVINYPKDIKSFYMKQNDDGKTVAAVDLLVPGIGELCGGSEREANLDKILERCEFYGINPEELDWYIGLRKYGFYKSAGFGLGFERLIMYITGASNIRDVIPFPRTPKTLLY" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(1813..1917) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAACGATGAT GCTCACTCTC GGGTCTCAAA TGGATATCAA 2041 GTTCCTGTAC GATAATCATG AAACCCTGAA GGAGCAAAAA GTGACCATCA TCGGCCGTGT 2101 CCGTTCCAAC CGCCAAGGCA AGGCCGTTAG CTTTATGGTG CTGAACGATG GTACCGTTCT 2161 GAACGATCTG CAAGTCGTCT ATAAGCCGGA AACGTTTGGT TTTGAGCAGG GTAGCAAGGC 2221 ACGCGTTTCT AGCATTGTTG AAATCGAAGG TATCCTGATT CCGACCCCGA CGCGTCCGCA 2281 ACCGTTCGAA ATCAATGCGA CCAGCATTAC GCTGCTGGAC CAGGCAATTG AAGAGTACCC 2341 GCTGCAGAAA AAAGAGCATA GCCCGGAGTT CCTGCGTGAG ATTAGCCATC TGCGTGCGCG 2401 TACCAAAACG TTCCAAGCCA TTTTCCGTAT TCGCTCTACT GCGGCGTTCG CAATCCATAA 2461 ATTCTTCCAA GAGAATAATT ACGTTTATGT TACCACGCCG ATCATTACGA GCAATGATGC 2521 CGAGGGCGCG GGCGAACAGT TTGTTGTGAC CGTGAACGAG GACAAGGACT ACGCAAATGA 2581 CTTTTTCGGT AAAAAGGCGA GCCTGACCGT TAGCGGCCAA CTGAATGGTG AAGCGTTCGC 2641 GCAAGCCTTT AAAAACATCT ATACTTTCGG TCCGACCTTT CGCGCCGAAA ATAGCCATAC 2701 CACTAAACAC GCCTCAGAAT TCTGGATGAT CGAGCCGGAA GTTGCATTTG CCGACATCAA 2761 CGACAACATC CAACTGATGC AGGATATGTT GAAAAGCGTG GTCGGCTACG TGCTGGAGAA 2821 AAATATGACG GAACTGGAAT TTCTGCAAGA ACAGCTGGAA CCGGGCCTGA TTGACAAAAT 2881 TACGGGTATC GTTAACGTTG AATTCAAGGT CAACACGTAT GAAGAGGTTT TAAAGACTCT 2941 GCAAGATGCG ATCGATAAAG GCCATAAGTT TGAAGAGAAC AACATCCATT TCGGTCTGGA 3001 CCTGGGTACC GAGCACGAGC GCTTTATTTG TGAAGAGGTC AATAAGTGCC CGACCTTCGT 3061 TATCAACTAT CCGAAAGACA TTAAAAGCTT TTACATGAAA CAGAACGACG ATGGTAAAAC 3121 CGTTGCAGCG GTTGATCTGC TGGTCCCGGG TATCGGCGAA CTGTGTGGCG GCAGCGAGCG 3181 TGAGGCGAAC CTGGATAAGA TTTTGGAGCG CTGTGAGTTC TACGGTATCA ATCCGGAGGA 3241 GCTGGATTGG TATATCGGCT TGCGTAAGTA CGGTTTCTAT AAAAGCGCGG GTTTCGGCCT 3301 GGGTTTCGAG CGCCTGATTA TGTACATCAC GGGTGCCAGC AACATTCGTG ATGTGATCCC 3361 GTTCCCACGC ACGCCGAAAA CCCTGCTGTA CTGAAGAGCT TAGAGACCCA TCCTTATGCG 3421 ATACGGGT //