LOCUS       Exported     3016 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3016
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1054..1101
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2949..2965
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2966..2997
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..982
                     /label="Mfl484"
                     /note="Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction."
                     /translation="MKILAIESSCDEFSISIIDDGKILTNIISSQIDQHVNFGGVVPELAARLHLENISWVIKSALESSNTKIEEIDHVAYTEKPGLIGSLIIGKLVAETIASYIDKPLMPLHHIEGHIYGASIENEFVYPVLAMVVSGGHTQIEIVNSPNEFEVIGATLDDAIGECYDKVARVMGLGYPGGPKIDKLAQKGNKEAFIFPISKNDDSYDFSYSGLKTAVINIIHNLTQKGEEIPVADIAASFQYAATKIVEKKLEKAIIQFKPKTLTVAGGVSANSEIRNIIMSLGKKYNITNTFVPKMEYCTDNAAMIAKLAYEKLKSSN"
     primer_bind     complement(1106..1122)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1189..1777)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /label="ori"
     CDS             complement(1968..2828)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
     promoter        complement(2829..2933)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAGGCCCAGA AGGATACAAC GGTCTCAAAT GAAAATTCTC GCCATCGAAT CTAGCTGTGA
       61 TGAGTTTTCG ATTAGCATTA TCGACGATGG CAAAATCCTG ACCAACATCA TTAGCAGCCA
      121 GATTGATCAA CACGTGAACT TCGGTGGCGT TGTTCCGGAG CTGGCGGCGC GCCTGCACCT
      181 GGAGAACATC TCCTGGGTTA TTAAGAGCGC ACTGGAATCT AGCAACACCA AGATTGAGGA
      241 GATTGACCAC GTCGCATATA CGGAAAAACC GGGTCTGATT GGTTCCCTGA TCATTGGTAA
      301 ACTGGTGGCG GAAACTATCG CGAGCTACAT TGACAAGCCG CTGATGCCGT TACATCACAT
      361 CGAGGGTCAT ATCTACGGCG CTAGCATTGA AAACGAGTTC GTCTACCCGG TGCTGGCCAT
      421 GGTTGTCAGC GGTGGCCACA CCCAAATTGA AATTGTTAAT AGCCCGAACG AGTTTGAGGT
      481 TATCGGTGCA ACGCTGGATG ACGCAATTGG CGAGTGTTAC GACAAGGTGG CACGCGTTAT
      541 GGGTCTGGGT TATCCGGGTG GCCCTAAAAT TGATAAACTG GCTCAGAAGG GTAACAAGGA
      601 AGCGTTTATT TTTCCAATCA GCAAGAACGA CGATAGCTAC GATTTTAGCT ATAGCGGCCT
      661 TAAGACGGCC GTAATCAACA TCATTCACAA CCTGACCCAG AAAGGCGAGG AGATTCCGGT
      721 TGCAGATATC GCGGCAAGCT TCCAATACGC AGCAACTAAA ATTGTAGAAA AGAAGCTGGA
      781 AAAGGCGATC ATTCAGTTTA AGCCAAAAAC GCTGACGGTC GCGGGTGGTG TCTCCGCCAA
      841 TAGCGAGATT CGTAACATTA TCATGTCCCT GGGCAAGAAG TATAACATTA CCAATACGTT
      901 CGTTCCAAAA ATGGAATACT GTACGGACAA CGCCGCTATG ATTGCCAAGC TGGCATACGA
      961 GAAACTTAAG AGCTCAAACT GAAGAGCTTA GAGACCCGAG TGTAGTTCCC GTCAATCGCT
     1021 GAGGTGTCAA TCGTCGGAGC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA
     1081 CGGGTCTTGA GGGGTTTTTT GCATGGTCAT AGCTGTTTCC TGAGAGCTTG GCAGGTGATG
     1141 ACACACATTA ACAAATTTCG TGAGGAGTCT CCAGAAGAAT GCCATTAATT TCCATAGGCT
     1201 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC
     1261 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC
     1321 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC
     1381 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG
     1441 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA
     1501 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
     1561 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
     1621 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
     1681 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
     1741 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GGCCTACTAT TAGCAACAAC
     1801 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG
     1861 ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA
     1921 AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA
     1981 ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC
     2041 CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG
     2101 ATACCGCGAG AACCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA
     2161 AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT
     2221 TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT
     2281 GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC
     2341 CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC
     2401 GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA
     2461 GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG
     2521 TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG
     2581 TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA
     2641 CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA
     2701 CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA
     2761 GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA
     2821 ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG
     2881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACCTGC
     2941 ACCAGTCAGT AAAACGACGG CCAGTAGTCA AAAGCCTCCG ACCGGAGGCT TTTGACTTGG
     3001 TCAGGTGGAG TGGGAG
//