LOCUS       Exported     3007 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3007
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1045..1092
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2940..2956
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2957..2988
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..973
                     /note=""
                     /translation="MTIYKQIKQVIEKYDKIIILRHIVPDGDAYGSQLGLKELIKTNYPNKEVYAFGEEIGYLKHAGTPDVFTDEAIFKDALVIVTDCGNVERIDNQNYDKGAFLLKIDHHPDATPYGDLSWVDVNYTSASEMVGDLAVNNNWEITPVAARVIYHGICTDSGRFLFGGITPRTFEVCARLIETGFDVFEMYKTMYKRSFPVLALQSELIASAKVTDHKVGYIILPDELMKKYNLSYEENGKFSNLLKDLEGIDIWITFSIREDGKWRVEFRSNGIAVNELAVKWGGGGHKMAAGAIINSLDEAMQIVEDANQIIIDNE"
                     /label="Mfl488"
     primer_bind     complement(1097..1113)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1180..1768)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1959..2819)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2820..2924)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAAGGGGCCG TCAATATCAG GGTCTCAAAT GACCATTTAT AAGCAGATCA AGCAAGTGAT
       61 CGAGAAGTAC GATAAAATCA TTATTCTGCG CCACATTGTT CCGGATGGTG ATGCCTATGG
      121 TAGCCAACTG GGTCTGAAGG AACTGATTAA AACCAACTAC CCGAATAAAG AGGTTTACGC
      181 GTTCGGTGAA GAGATTGGTT ACCTGAAACA TGCCGGTACG CCTGATGTGT TCACCGATGA
      241 GGCGATTTTT AAAGACGCGT TGGTCATCGT GACTGATTGC GGTAATGTCG AACGTATCGA
      301 TAATCAAAAC TATGATAAAG GCGCCTTCCT GCTGAAAATT GACCACCATC CGGACGCAAC
      361 GCCGTACGGT GATCTGAGCT GGGTGGATGT CAACTACACC AGCGCGAGCG AGATGGTTGG
      421 TGACTTGGCG GTCAACAACA ATTGGGAGAT TACGCCGGTT GCGGCACGTG TTATTTACCA
      481 CGGTATCTGC ACCGATAGCG GTCGCTTTCT GTTCGGCGGT ATTACGCCGC GCACCTTTGA
      541 AGTGTGCGCG CGTCTGATTG AAACCGGCTT CGATGTTTTT GAAATGTACA AAACCATGTA
      601 CAAGCGCTCT TTCCCGGTGC TGGCGCTGCA AAGCGAGCTG ATCGCGAGCG CGAAAGTCAC
      661 CGACCACAAA GTGGGTTACA TTATTCTTCC AGACGAATTA ATGAAAAAAT ATAACCTGTC
      721 CTACGAGGAA AATGGTAAAT TTAGCAATCT GCTGAAAGAC TTGGAGGGTA TTGACATTTG
      781 GATCACCTTT AGCATCCGCG AAGATGGTAA ATGGCGTGTT GAGTTCCGTT CCAACGGTAT
      841 CGCCGTTAAT GAACTGGCCG TTAAGTGGGG TGGCGGCGGC CATAAAATGG CGGCGGGTGC
      901 GATCATTAAC AGCCTGGACG AAGCCATGCA AATCGTTGAG GATGCCAACC AAATTATCAT
      961 CGACAACGAG TGAAGAGCTT AGAGACCGCC ATCCTCTTAT CTCGTGGCGC TGAGGTGTCA
     1021 ATCGTCGGAG CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG
     1081 AGGGGTTTTT TGCATGGTCA TAGCTGTTTC CTGAGAGCTT GGCAGGTGAT GACACACATT
     1141 AACAAATTTC GTGAGGAGTC TCCAGAAGAA TGCCATTAAT TTCCATAGGC TCCGCCCCCC
     1201 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA
     1261 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC
     1321 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC
     1381 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA
     1441 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC
     1501 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG
     1561 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG
     1621 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG
     1681 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA
     1741 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGGCCTACTA TTAGCAACAA CGATCCTTTG
     1801 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     1861 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     1921 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     1981 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     2041 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     2101 GAACCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     2161 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     2221 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     2281 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     2341 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     2401 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     2461 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     2521 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     2581 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     2641 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     2701 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     2761 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     2821 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     2881 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACCTG CACCAGTCAG
     2941 TAAAACGACG GCCAGTAGTC AAAAGCCTCC GACCGGAGGC TTTTGACTTG GTCAGGTGGA
     3001 GTGGGAG
//