LOCUS       Exported     3740 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3740
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3706
                     /label="Mfl489"
                     /note=""
                     /translation="MRKTVRLVILLLVVAVIYFGYSAWIDSVAIYNIGAVQLGSGTNWNKTLDDLIMVAGLSESNAWTAFFEKYTDLNAAMLFNGGTLPSGIITSSALSLGGSTNIAFINALIEAANDKAGAGTYVLNGFMAANSLLKTLLDPFKMILVGGVFAMFVPLTKQLLFGTVIGMKSYMKNRQSNVLYNYQKTISFTEGLQVKLAEGNFDAVKADYAAYSGLAFKPEFLNIMMDEICSVLIKEGDLTVFAEAAKVVTESIKEMYEKERRRSINGRGDELFFDLKRGYEYCATGSKYSIAYHKALQDKADNNLGWKIYSLEIFKFNFFLVAMFIPAIIISAFVGGLINGLVSNLTGDVRTLAVAATFFVSWVILTIIAHSLMIFRNEQYKNIKNILVKPAIIFYSLYVLTFITISAGIVGVSKVNIVAPGSSAQIWVWFSAVAYLVLSTSLIFYVIATLVDSFKQHKTLSKKLLVDGIILPMIAWTIATVSNFMALYGPDGHSLQSIFSAINGVTLVGFWIYLSISSFLINNLISPNMARELRKAVEADALKNAKAKKSTKSTKAAK"
     primer_bind     complement(90..106)
                     /note="common sequencing primer, one of multiple similar variants"
                     /label="M13 rev"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAGGAACTA TGGCATCGAG CGGTCTCAAA TGCGTAAGAC
     2041 CGTCCGTCTG GTGATTTTGT TGCTGGTTGT TGCTGTCATC TATTTCGGTT ACAGCGCGTG
     2101 GATTGACAGC GTGGCGATCT ACAACATTGG CGCCGTCCAA CTGGGTAGCG GTACCAATTG
     2161 GAACAAAACC CTGGACGATC TGATCATGGT CGCCGGTCTG AGCGAGAGCA ACGCCTGGAC
     2221 CGCGTTCTTC GAGAAGTACA CCGATCTCAA CGCCGCTATG CTGTTCAATG GTGGCACCCT
     2281 GCCAAGCGGC ATTATCACCA GCAGCGCCCT GAGCCTGGGT GGTAGCACTA ACATTGCGTT
     2341 CATTAACGCC CTGATCGAGG CGGCGAATGA CAAAGCGGGC GCAGGCACCT ACGTTTTGAA
     2401 TGGCTTTATG GCGGCGAATT CTCTGCTGAA AACCCTGCTG GATCCGTTCA AGATGATCTT
     2461 GGTCGGTGGC GTGTTTGCAA TGTTCGTTCC GCTGACCAAG CAATTGCTGT TCGGCACTGT
     2521 TATCGGTATG AAAAGCTATA TGAAAAACCG CCAGTCTAAT GTTCTGTATA ACTACCAGAA
     2581 AACCATTAGC TTCACCGAGG GTCTGCAAGT TAAGTTGGCA GAGGGCAATT TCGACGCAGT
     2641 TAAAGCTGAT TACGCGGCCT ATAGCGGTCT GGCCTTTAAG CCGGAATTTC TGAACATCAT
     2701 GATGGACGAA ATTTGCTCTG TGCTGATCAA AGAGGGTGAC CTGACGGTTT TTGCAGAAGC
     2761 GGCGAAAGTC GTAACCGAGA GCATTAAAGA GATGTACGAA AAAGAACGCC GTCGTAGCAT
     2821 TAACGGTCGC GGTGATGAGC TGTTCTTCGA CCTGAAACGT GGTTACGAAT ACTGTGCGAC
     2881 CGGCAGCAAA TACTCTATCG CGTATCATAA AGCCCTGCAG GATAAAGCTG ACAACAATTT
     2941 GGGCTGGAAG ATCTACAGCC TGGAGATTTT TAAATTCAAC TTCTTTTTGG TGGCTATGTT
     3001 CATTCCGGCC ATCATCATTA GCGCGTTCGT GGGTGGCCTG ATTAACGGTC TGGTTAGCAA
     3061 TCTGACGGGT GACGTGCGTA CCCTGGCAGT GGCAGCGACT TTTTTCGTTT CCTGGGTCAT
     3121 TCTCACTATT ATCGCGCACT CCTTGATGAT TTTCCGCAAC GAGCAATACA AGAATATTAA
     3181 GAACATTCTG GTGAAGCCGG CTATCATCTT TTACTCTCTG TACGTTCTGA CCTTTATCAC
     3241 GATCTCTGCG GGTATCGTTG GCGTCAGCAA AGTCAATATT GTCGCGCCGG GTAGCAGCGC
     3301 TCAAATTTGG GTGTGGTTCA GCGCCGTCGC GTACCTGGTT TTGTCCACCA GCCTGATTTT
     3361 CTACGTTATT GCGACTCTGG TGGACAGCTT CAAACAACAC AAGACGCTGA GCAAAAAACT
     3421 GTTGGTCGAC GGCATCATTC TGCCGATGAT TGCCTGGACT ATTGCAACGG TCAGCAATTT
     3481 TATGGCGCTG TATGGTCCAG ATGGTCACAG CTTGCAGAGC ATTTTTAGCG CAATCAATGG
     3541 TGTCACCTTG GTGGGCTTCT GGATCTACCT GTCCATCAGC TCCTTTCTGA TCAATAATCT
     3601 GATCAGCCCA AACATGGCCC GTGAGCTGCG TAAAGCTGTT GAGGCAGACG CACTGAAAAA
     3661 CGCAAAAGCG AAGAAAAGCA CCAAGAGCAC CAAGGCTGCC AAGTGAAGAG CTTAGAGACC
     3721 GGACCTTGAC GCCTAATACT
//