LOCUS       Exported     3832 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3832
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1870..1917
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     3765..3781
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      3782..3813
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
                     /label="tonB terminator"
     CDS             29..1798
                     /label="Mfl491"
                     /note=""
                     /translation="MNQSFRIYKLDTKKIWENELQRHNEEMNLSIIKDLIFQYIIDKNSLVENNFWIYNEESDDENIAALVYIKDAEISWMSGLSNFFKDQHFDNLVNTSVSFIFLIAYENELYASTGGHASNYISEFIVKDFGKEIITRLYDGTEPVLQKFVEKPIRGKRIQSTNYNRSNSTITSEIQFSNNIKNLDLETTFNVSKELGIIDEGTSEDEFSKNKINAADFFEIKKSVSPQEFKKYISSISSISKKEPKLWLSYWKPVSEFAENGLNEKYFDEFYEIFETIDKKEITITWSDYADYFNAAKWIIESRDGKFRKEYDVIPTFENIYSDFVKFNFEKAPNRDVSFSKNKIRNFFMNWEITIFDEENKEIKKQKLKNIIDYIYITQEGEIYLNGGKWYILDQTYDEHIKLSFKKLNEKSKKELEDVYKAKKLFKNKSHRINEKKYNDSFLSKKDNKDIILADRITIKNVEICDLIVFADNDQTYLICNKASPTGIGSRDLFNQIETSANLIHSAQNVIYDYYDKLFEKNRIPENISKEDFFKKIRNAKYVAAFVTAPLKWNTESFYSKFLFVDTENVLKTYNKELFLYSPAYEEEI"
     primer_bind     complement(1922..1938)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(2005..2593)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(2784..3644)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(3645..3749)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AATGTCCTTA GGCAGTCGTC GGTCTCAAAT GAATCAGAGC TTTCGCATTT ACAAACTGGA
       61 CACGAAGAAG ATCTGGGAAA ACGAACTGCA GCGTCACAAT GAAGAGATGA ACCTGAGCAT
      121 CATTAAAGAT CTGATTTTTC AGTATATCAT CGACAAAAAC AGCCTGGTCG AGAACAATTT
      181 TTGGATCTAC AATGAGGAAT CTGATGATGA AAATATCGCG GCGCTGGTGT ACATTAAGGA
      241 CGCAGAGATT TCTTGGATGA GCGGCCTGAG CAACTTTTTC AAAGATCAGC ACTTCGATAA
      301 CCTGGTTAAT ACCAGCGTCA GCTTTATTTT CCTGATCGCA TATGAAAATG AACTGTATGC
      361 TAGCACCGGT GGTCATGCGA GCAACTACAT TTCGGAATTT ATCGTGAAGG ATTTTGGTAA
      421 AGAGATCATT ACCCGCCTGT ATGACGGCAC GGAACCGGTT TTACAAAAGT TTGTTGAGAA
      481 ACCGATTCGT GGTAAGCGTA TTCAGTCCAC CAATTACAAT CGCAGCAACA GCACCATCAC
      541 CTCAGAGATC CAATTCTCCA ACAACATCAA AAATTTAGAC CTGGAAACTA CCTTCAACGT
      601 CAGCAAAGAA CTGGGCATCA TCGACGAAGG CACCAGCGAA GATGAGTTTA GCAAAAATAA
      661 GATCAACGCA GCGGATTTTT TCGAAATTAA AAAATCCGTG TCCCCGCAAG AGTTCAAAAA
      721 ATACATCAGC TCAATTAGCA GCATCAGCAA GAAAGAACCA AAGCTGTGGT TGAGCTATTG
      781 GAAGCCGGTG AGCGAGTTCG CAGAGAACGG TCTGAACGAG AAATACTTCG ATGAGTTCTA
      841 TGAAATTTTC GAAACGATTG ACAAAAAGGA AATCACGATT ACGTGGTCTG ATTACGCAGA
      901 TTATTTCAAC GCGGCAAAAT GGATCATCGA GTCTCGTGAC GGTAAGTTCC GCAAAGAGTA
      961 TGACGTGATT CCGACGTTTG AGAACATCTA CAGCGACTTT GTTAAATTCA ACTTTGAAAA
     1021 GGCGCCGAAT CGTGACGTTA GCTTTAGCAA AAACAAAATT CGTAATTTCT TTATGAATTG
     1081 GGAGATTACG ATTTTTGACG AGGAGAACAA AGAGATCAAA AAACAAAAGC TGAAAAACAT
     1141 CATCGACTAC ATCTACATCA CCCAGGAGGG TGAGATTTAT CTGAACGGTG GTAAATGGTA
     1201 CATCTTGGAC CAAACGTATG ATGAACACAT CAAACTGAGC TTCAAGAAAC TGAACGAAAA
     1261 GAGCAAGAAA GAACTGGAGG ACGTGTACAA GGCGAAGAAG CTGTTTAAGA ACAAAAGCCA
     1321 TCGTATTAAC GAAAAGAAAT ACAATGATTC TTTCCTGAGC AAAAAGGATA ACAAGGATAT
     1381 TATTCTGGCG GATCGCATTA CTATCAAGAA TGTTGAGATT TGCGATTTGA TCGTGTTCGC
     1441 TGACAACGAC CAGACCTATC TGATTTGTAA TAAAGCATCT CCGACGGGCA TTGGTAGCCG
     1501 TGATCTGTTT AACCAGATCG AAACGAGCGC GAATCTGATC CATTCCGCGC AGAATGTGAT
     1561 CTACGATTAC TACGACAAGC TGTTCGAGAA AAACCGTATC CCGGAGAATA TTAGCAAGGA
     1621 GGACTTTTTC AAGAAAATCC GCAACGCGAA GTATGTCGCG GCTTTCGTCA CCGCCCCGCT
     1681 CAAATGGAAC ACCGAAAGCT TTTATAGCAA GTTCCTGTTC GTGGACACCG AAAATGTGCT
     1741 CAAGACGTAC AACAAGGAGC TGTTTCTGTA TTCGCCGGCG TATGAGGAAG AAATTTGAAG
     1801 AGCTTAGAGA CCGTACGTCT GAACTTGGGA CTCGCTGAGG TGTCAATCGT CGGAGCCGCT
     1861 GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCAT
     1921 GGTCATAGCT GTTTCCTGAG AGCTTGGCAG GTGATGACAC ACATTAACAA ATTTCGTGAG
     1981 GAGTCTCCAG AAGAATGCCA TTAATTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA
     2041 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT
     2101 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT
     2161 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT
     2221 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC
     2281 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT
     2341 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC
     2401 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT
     2461 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA
     2521 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA
     2581 AAAAGGATCT CAAGAAGGCC TACTATTAGC AACAACGATC CTTTGATCTT TTCTACGGGG
     2641 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA
     2701 AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA
     2761 TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG
     2821 ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA
     2881 CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGAACC ACGCTCACCG
     2941 GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT
     3001 GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT
     3061 TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC
     3121 TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA
     3181 TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT
     3241 AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC
     3301 ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA
     3361 TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA
     3421 CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA
     3481 AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT
     3541 TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC
     3601 GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA
     3661 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT
     3721 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACCTGCACCA GTCAGTAAAA CGACGGCCAG
     3781 TAGTCAAAAG CCTCCGACCG GAGGCTTTTG ACTTGGTCAG GTGGAGTGGG AG
//