LOCUS       Exported     3010 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3010
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      1048..1095
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     2943..2959
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      2960..2991
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             29..976
                     /translation="MKKTKMTYHDIAKKAGVGIGTVSRYFNDYNISEEAKSKINKVLNETDYVPNFAASNIKKPSKDVYLILPYNNDETANMEIVNGVKSSLTADNINFFIFLSSSESEIYQKDLKYLTMRNPFGIVLLLPKHTSKDLILQIQDIKSTNLIVYNRNIEGVKSVNIDDKEMFKELALKIETDYKNEKVAFIGLNKEDITTGKYRSESFTENIKNNKVQNYLLKQNSFEDVEEIIHKIIKDYNPKIIVSATHTISMYVYGHLMENKIRNNFITTDIGRMGKSQYLTNSDINIFIDYFLIGYQIGNKLLNKEKKMENFFKII"
                     /label="Mfl501"
                     /note=""
     primer_bind     complement(1100..1116)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(1183..1771)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(1962..2822)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(2823..2927)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 AAACACGTGG CAAACATTCC GGTCTCAAAT GAAAAAGACC AAGATGACCT ATCATGATAT
       61 CGCCAAAAAA GCGGGTGTCG GCATTGGCAC GGTTTCTCGT TATTTCAACG ATTACAATAT
      121 CAGCGAAGAA GCAAAAAGCA AGATTAATAA AGTGTTGAAC GAAACGGATT ATGTTCCGAA
      181 TTTCGCCGCG AGCAACATCA AAAAGCCGAG CAAGGATGTT TACCTGATTC TGCCGTACAA
      241 TAACGACGAA ACGGCAAATA TGGAGATCGT CAATGGTGTT AAGAGCAGCC TTACTGCAGA
      301 TAACATCAAC TTCTTTATCT TTCTGAGCTC CAGCGAAAGC GAGATCTACC AAAAAGATCT
      361 GAAGTATCTG ACCATGCGCA ATCCGTTCGG TATTGTGCTG TTGCTGCCAA AACATACCAG
      421 CAAGGATCTG ATTCTGCAAA TTCAGGACAT TAAAAGCACC AATCTGATCG TCTACAACCG
      481 CAATATTGAG GGCGTTAAAA GCGTCAACAT TGACGACAAG GAAATGTTCA AAGAGCTGGC
      541 ACTGAAAATC GAAACGGATT ATAAGAATGA GAAGGTTGCG TTCATCGGCT TGAACAAGGA
      601 GGATATCACG ACCGGTAAGT ACCGCAGCGA ATCGTTCACC GAGAACATCA AGAATAATAA
      661 AGTGCAAAAT TACCTGTTGA AACAAAACAG CTTTGAGGAT GTCGAAGAGA TTATTCACAA
      721 AATTATTAAA GACTACAATC CGAAGATTAT CGTGAGCGCG ACGCATACCA TCAGCATGTA
      781 TGTCTACGGC CACCTGATGG AGAACAAAAT CCGTAACAAC TTCATTACGA CCGATATCGG
      841 CCGCATGGGT AAGAGCCAAT ACCTGACGAA CAGCGACATC AATATTTTCA TCGACTACTT
      901 TCTGATCGGT TATCAGATCG GCAACAAACT GCTGAATAAA GAGAAGAAGA TGGAAAACTT
      961 CTTTAAAATT ATTTGAAGAG CTTAGAGACC GCCACGGCGA AATTTAATGT CGCTGAGGTG
     1021 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC
     1081 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC
     1141 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC
     1201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT
     1261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT
     1321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG
     1381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA
     1441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA
     1501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC
     1561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG
     1621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG
     1681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA
     1741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT
     1801 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG
     1861 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT
     1921 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT
     1981 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC
     2041 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG
     2101 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC
     2161 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG
     2221 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA
     2281 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA
     2341 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT
     2401 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG
     2461 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA
     2521 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA
     2581 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT
     2641 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT
     2701 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA
     2761 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC
     2821 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA
     2881 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT
     2941 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT
     3001 GGAGTGGGAG
//