LOCUS Exported 3662 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3662 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 38..85 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 1933..1949 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 1950..1981 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 2030..3628 /label="Mfl502" /note="Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate." /translation="MNVKKPVILAILDGWGIEEAGVGNAVANADQKFVKEMMGMYPWVKAHASGEWVGLPEGQMGNSEVGHIHLGAGRINMESLAKLNHEVKVDGFLTNEVLVDTFKYVKEHNSALHLMGLFSDGGVHSHMNHMISMYKAAVKFGLTNIKFDLITDGRDTAPKVAEQYINQLLQVIKDNNNIGEIASISGRYFAMDRDKRFERSAAAYITMTERKVDQPKFTDPIEYVKAAYENGLDDEMIVPAYNASVVDSELKANDAMIFTNFRPDRAIQMASIMTNNNYPAWNDEAFKDVEFIGDKIRFVSTMKYADSVTSQFIAYPPTPLTNTLGEYISSLGLKQLRIAETEKIAHVTFFFDGGNDYFKNGLAKPEEISLPHASIDLISSPKVATYDLKPEMSAVEITDKLLEEVKKDEFDLIVLNFANCDMVGHTGNNDATVKGVKVLDEQLKRIHDEFVLKHNGVMVITADHGNAEIMIDETGGPNKKHTTSLVPIIVTDKTIELSDFDPAIAKVAPTILDIMGLEIPKEMTQPSMIIKK" primer_bind complement(90..106) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(173..761) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(952..1812) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(1813..1917) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC 61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT 121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA 181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC 241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC 361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG 421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC 481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA 541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA 661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG 721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA 781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG 961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG 1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC 1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC 1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA 1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC 1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG 1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC 1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT 1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG 1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC 1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG 1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT 1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG 1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG 1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC 1981 TTGGTTCAGG TGGAGTGGGA GAATGCAAAG CTATTAGCGC GGGTCTCAAA TGAATGTGAA 2041 AAAGCCGGTC ATTTTGGCTA TTCTGGATGG TTGGGGTATC GAGGAGGCCG GCGTCGGCAA 2101 CGCGGTGGCG AATGCTGATC AGAAGTTCGT CAAGGAGATG ATGGGTATGT ATCCGTGGGT 2161 GAAAGCACAC GCTAGCGGCG AGTGGGTGGG TCTGCCAGAG GGCCAGATGG GTAACAGCGA 2221 AGTGGGTCAT ATCCACCTGG GCGCCGGTCG TATTAATATG GAAAGCCTGG CAAAACTGAA 2281 TCACGAGGTT AAAGTTGATG GTTTTCTGAC TAACGAAGTG CTGGTGGACA CCTTCAAGTA 2341 TGTTAAGGAA CACAATAGCG CGTTACACTT GATGGGCCTC TTCAGCGACG GTGGTGTGCA 2401 TTCCCACATG AACCACATGA TCAGCATGTA TAAAGCAGCA GTTAAGTTCG GTCTGACCAA 2461 CATCAAGTTT GATTTGATCA CCGACGGTCG TGATACCGCA CCGAAAGTCG CGGAACAATA 2521 TATTAACCAG CTGTTGCAAG TGATCAAGGA CAATAACAAC ATCGGCGAAA TTGCGAGCAT 2581 CAGCGGCCGT TACTTCGCTA TGGATCGTGA TAAACGCTTT GAGCGTAGCG CGGCGGCCTA 2641 CATTACGATG ACGGAACGTA AGGTCGACCA GCCGAAATTT ACTGATCCGA TCGAATATGT 2701 TAAAGCAGCT TACGAGAATG GCCTGGACGA CGAGATGATC GTCCCGGCGT ATAATGCGAG 2761 CGTGGTGGAC AGCGAGCTGA AAGCAAACGA TGCTATGATC TTCACCAACT TTCGCCCGGA 2821 TCGCGCGATC CAGATGGCCA GCATTATGAC CAACAACAAC TACCCGGCGT GGAATGATGA 2881 GGCGTTTAAG GATGTTGAAT TCATCGGTGA TAAAATTCGC TTTGTGTCCA CGATGAAATA 2941 CGCCGATAGC GTCACCAGCC AGTTCATTGC ATATCCACCT ACTCCGCTGA CCAACACGCT 3001 GGGTGAGTAT ATTTCCAGCC TGGGCCTGAA GCAACTTCGC ATTGCGGAAA CCGAGAAAAT 3061 CGCACACGTG ACCTTCTTTT TCGATGGCGG TAATGACTAC TTTAAAAATG GTCTGGCGAA 3121 GCCGGAAGAG ATCTCTCTGC CGCACGCGAG CATCGATCTG ATCAGCAGCC CGAAAGTCGC 3181 AACCTACGAC CTGAAACCGG AGATGAGCGC GGTTGAAATT ACCGATAAGC TGTTGGAAGA 3241 AGTGAAGAAG GATGAATTCG ATCTGATCGT GCTGAATTTC GCGAATTGTG ATATGGTCGG 3301 CCACACCGGT AACAACGACG CGACGGTCAA GGGTGTGAAG GTGCTGGATG AACAACTGAA 3361 GCGTATTCAC GATGAGTTCG TTCTGAAGCA CAATGGTGTC ATGGTTATCA CTGCGGATCA 3421 CGGCAATGCT GAAATCATGA TTGACGAAAC CGGTGGCCCG AATAAGAAAC ATACGACGAG 3481 CCTGGTTCCG ATTATTGTCA CGGACAAAAC CATTGAGCTG TCCGACTTCG ATCCTGCCAT 3541 TGCAAAAGTC GCGCCGACGA TTCTGGACAT TATGGGCTTG GAAATTCCGA AAGAAATGAC 3601 GCAACCGAGC ATGATCATTA AAAAGTGAAG AGCTTAGAGA CCGCTTGACG GAGCATCTAT 3661 CT //