LOCUS Exported 2890 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..2890 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 928..975 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2823..2839 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2840..2871 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..856 /translation="MLLSFLFHWFSYCYIIKNVQKNKRRNIMRQKVIFGNWKMNGTNAELVTFLKKVDKAAKKSDVVAGLGLPFTLLSKGIEKAKNVKVAAQNVHFAEKGAFTGEVSISMLQEVGVQYVIIGHSERREMFAETDETVNKKATALLAAGITPIICCGETLETKEAKKTVTFVNAQIKKAYKGISAEDALKTIIAYEPIWAIGTGKTATSEDAEKVCEAIRKNLTKIYDEKTAQQITIQYGGSVNPSNIAELMSQPNIDGALVGGASLKADDFIALINYKK" /label="tpiA" /note="Involved in the gluconeogenesis. Catalyzes stereospecifically the conversion of dihydroxyacetone phosphate (DHAP) to D-glyceraldehyde-3-phosphate (G3P)." primer_bind complement(980..996) /label="M13 rev" /note="common sequencing primer, one of multiple similar variants" rep_origin complement(1063..1651) /label="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1842..2702) /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" /label="bla" promoter complement(2703..2807) /label="bla promoter" /note="ampicillin resistance gene promoter" ORIGIN 1 AAACCGGAGC CATACAGTAC GGTCTCAAAT GTTGCTGAGC TTTCTGTTTC ACTGGTTTTC 61 TTATTGCTAC ATTATTAAAA ATGTGCAGAA AAACAAACGT CGCAATATCA TGCGTCAGAA 121 GGTGATTTTT GGCAATTGGA AGATGAACGG TACCAACGCC GAGCTGGTCA CCTTCCTCAA 181 GAAGGTTGAC AAAGCGGCAA AAAAGTCCGA TGTCGTCGCG GGCCTGGGTC TGCCATTCAC 241 GTTGCTGTCT AAAGGCATCG AGAAAGCGAA GAATGTTAAA GTGGCGGCCC AGAATGTGCA 301 CTTTGCGGAG AAGGGCGCTT TTACGGGTGA GGTTAGCATC TCTATGCTGC AGGAGGTGGG 361 TGTGCAATAC GTTATCATTG GTCACAGCGA GCGTCGCGAG ATGTTTGCTG AAACTGATGA 421 AACCGTGAAC AAAAAGGCTA CCGCGTTGCT GGCTGCAGGC ATCACCCCGA TTATTTGCTG 481 CGGTGAAACG CTGGAAACCA AAGAAGCGAA GAAAACCGTC ACGTTCGTTA ATGCGCAGAT 541 TAAGAAGGCC TACAAGGGTA TCAGCGCCGA GGACGCGCTT AAGACCATTA TCGCGTACGA 601 GCCGATTTGG GCAATTGGTA CCGGTAAGAC CGCTACGTCC GAGGACGCGG AAAAGGTCTG 661 TGAGGCTATC CGCAAAAATC TCACCAAAAT TTACGATGAA AAGACCGCGC AGCAGATCAC 721 CATCCAGTAT GGTGGTAGCG TGAATCCGTC CAATATTGCT GAACTGATGA GCCAGCCGAA 781 TATTGATGGC GCCCTGGTTG GCGGCGCGAG CCTGAAGGCA GACGATTTCA TTGCACTGAT 841 TAACTATAAG AAGTGAAGAG CTTAGAGACC CGCTAAGATC CGTAGGTCTG CGCTGAGGTG 901 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 961 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1021 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1081 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1141 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1201 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1261 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1321 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1381 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1441 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1501 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 1561 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 1621 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 1681 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 1741 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 1801 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 1861 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 1921 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 1981 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 2041 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 2101 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 2161 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 2221 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 2281 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 2341 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 2401 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 2461 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 2521 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 2581 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 2641 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 2701 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 2761 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 2821 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT 2881 GGAGTGGGAG //