LOCUS Exported 3010 bp DNA circular SYN 24-JUL-2020 DEFINITION synthetic linear DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2001) AUTHORS Keoni Gandall TITLE Direct Submission JOURNAL Exported from Poly. FEATURES Location/Qualifiers terminator 1..3010 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" terminator 1048..1095 /label="T7 terminator" /note="transcription terminator for bacteriophage T7 RNA polymerase" primer_bind 2943..2959 /label="M13 for" /note="common sequencing primer, one of multiple similar variants" terminator 2960..2991 /label="tonB terminator" /note="bidirectional E. coli tonB-P14 transcription terminator" CDS 29..976 /label="Mfl508" /note="" /translation="MFFTLKKEKLTAWYKNYTLKDWRFWYKTLFVVLMTFIVLFSYIQLFVNMSSVVKQINDLNLSSDIASDKLKEVLDNLNLSKSIQERLIFYKTSDGLLHAGYQPFEQFIQMSSFFTLISNLLILIWMYVALFKPLNEGKKGILNKRTSIIFAAYITVTFLLYNLILRITVSEIANNFMSHFVNEMFHTVAPIFFIGYVLFGVKWEKGTAFNFKELSTTWVFGILGLCSYGAYAIIRGLLKVAGGTPGSSQQAFPYPFLQVTELNVKMGAIELPGIVLFLIFMIVIASICIGFTSLYNFIIIKNANKGAKKGVNNEK" primer_bind complement(1100..1116) /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" rep_origin complement(1183..1771) /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" CDS complement(1962..2822) /label="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*" promoter complement(2823..2927) /note="ampicillin resistance gene promoter" /label="bla promoter" ORIGIN 1 AAGACGATCC GAGCCATTAC GGTCTCAAAT GTTTTTTACC CTGAAGAAGG AAAAACTGAC 61 GGCTTGGTAT AAAAACTACA CGTTGAAGGA CTGGCGTTTC TGGTATAAGA CGCTGTTTGT 121 CGTGCTGATG ACCTTCATCG TTTTGTTTAG CTACATTCAA CTGTTCGTTA ATATGAGCAG 181 CGTTGTTAAA CAAATTAACG ACCTGAATCT GTCCTCTGAT ATTGCCTCCG ACAAACTGAA 241 AGAGGTGCTG GATAACCTCA ACCTGAGCAA GTCCATCCAG GAGCGCCTGA TCTTCTATAA 301 AACGTCCGAT GGTCTGCTGC ACGCCGGCTA CCAGCCGTTT GAGCAATTCA TCCAAATGAG 361 CTCTTTCTTT ACGTTGATTA GCAACTTGCT GATCCTGATC TGGATGTACG TGGCGCTGTT 421 CAAGCCACTG AATGAAGGTA AAAAAGGCAT TCTGAACAAG CGTACCTCCA TTATTTTCGC 481 GGCGTACATC ACCGTGACCT TTCTGCTGTA CAATCTGATC CTGCGCATTA CCGTTAGCGA 541 AATTGCGAAC AATTTCATGT CCCACTTCGT GAATGAAATG TTCCACACGG TTGCTCCGAT 601 CTTTTTCATC GGTTATGTCC TGTTCGGCGT TAAATGGGAG AAGGGTACGG CATTTAACTT 661 CAAAGAATTA AGCACCACGT GGGTTTTTGG CATCTTGGGT CTGTGCAGCT ACGGTGCGTA 721 CGCGATCATC CGTGGTCTGC TGAAGGTTGC GGGTGGTACG CCGGGCAGCA GCCAGCAGGC 781 CTTCCCGTAT CCGTTTCTGC AAGTGACCGA GCTGAATGTG AAGATGGGTG CCATTGAACT 841 GCCGGGCATC GTCCTGTTCC TGATCTTCAT GATCGTGATT GCCAGCATCT GCATCGGCTT 901 TACGTCGCTG TATAACTTTA TTATCATTAA GAACGCGAAC AAGGGTGCCA AAAAGGGTGT 961 TAACAATGAA AAGTGAAGAG CTTAGAGACC CTTGAAATCT TGTCGCACGG CGCTGAGGTG 1021 TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1081 TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT GATGACACAC 1141 ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA GGCTCCGCCC 1201 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 1261 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 1321 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 1381 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 1441 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 1501 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 1561 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 1621 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 1681 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 1741 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA CAACGATCCT 1801 TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG 1861 GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT 1921 AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT 1981 GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC 2041 GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG 2101 CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC 2161 GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG 2221 GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA 2281 GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA 2341 TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT 2401 CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG 2461 CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA 2521 ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA 2581 CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT 2641 TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT 2701 CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA 2761 ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC 2821 ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 2881 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC CTGCACCAGT 2941 CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC TTGGTCAGGT 3001 GGAGTGGGAG //