LOCUS       Exported     3782 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3782
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3748
                     /label="Mfl516"
                     /note=""
                     /translation="MKKKDYAFEAKRFIKAVGGKSNIESYLHCVTRLRLNLIDKTKVNEAEIKASPLTKGINYSQNQLQIILGSGVVEAVYEEVQKEMEVSNLSLDEKLSNDKLEDFKLQAKANKEALRNKSGFFGQIQKGMRVLGDIFVPIIPAIVAAGLAMGVAALLKRILNPSGAELNSLLWIIVDIITKTAFDSLAVLVCWSTVKRFGGNPVLGIIIGLMLVSPLLPNKGSIAAYNAQLDFFNDQSSKGLNINQIYDYWNNAGLKWMGTAPIIDDESKEILVNATMGAPITAIKFWIIPITGYQGSVLPALIIGIVVAYLEKWIKSWMPKSVNMIFTPFLTISFALLIALFFFGPILLMVEQGILIATKAILGIKYGFGTAIIAGLLQGIVITGCHQVLQGLEMQLVIDGSLPGANGADPAGSIFNAIWTASIISQGGAAMAIAIKAKDKSDKELGFSAALSTFFGITEPAIFGTNLPKVKPFIYASIGAFFGGLFAGALNVTCPGMGVTVVPGLLLYTGDWVKLLGIIGVNIIAFSSAFLLTFFLFKEGVEAKKTKLGLKFDELKAKVKFDKIKFRKNKAE"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /note="ampicillin resistance gene promoter"
                     /label="bla promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCAAA TGAAAAAGAA
     2041 GGACTACGCA TTTGAAGCAA AGCGTTTCAT TAAAGCAGTC GGTGGCAAGT CTAACATCGA
     2101 ATCGTATCTG CACTGTGTTA CTCGTCTGCG TCTGAACCTG ATTGACAAGA CGAAAGTCAA
     2161 CGAAGCGGAA ATTAAGGCGA GCCCGCTGAC CAAAGGCATC AACTATAGCC AAAATCAGCT
     2221 GCAAATTATC TTGGGCAGCG GCGTGGTCGA GGCGGTGTAT GAGGAAGTTC AAAAGGAAAT
     2281 GGAAGTGAGC AACCTGAGCC TGGATGAGAA ACTGTCGAAC GATAAACTGG AGGACTTCAA
     2341 GCTGCAGGCG AAGGCTAACA AAGAAGCTCT GCGTAACAAG TCTGGTTTTT TCGGTCAAAT
     2401 CCAAAAGGGT ATGCGTGTTC TGGGTGATAT CTTTGTGCCT ATTATCCCGG CCATCGTCGC
     2461 GGCCGGTTTG GCTATGGGTG TTGCTGCACT GCTGAAGCGC ATCCTGAACC CGAGCGGTGC
     2521 GGAACTGAAC AGCCTGTTGT GGATTATTGT GGATATCATC ACCAAAACTG CATTCGATAG
     2581 CCTGGCGGTC CTGGTTTGCT GGAGCACCGT TAAGCGCTTC GGTGGCAACC CAGTGCTGGG
     2641 CATCATTATT GGTCTGATGC TGGTCAGCCC GCTGCTGCCG AATAAGGGCT CTATCGCGGC
     2701 TTATAATGCC CAACTGGACT TTTTTAACGA TCAATCCAGC AAAGGTTTGA ACATCAACCA
     2761 AATTTACGAC TATTGGAACA ATGCGGGCCT GAAGTGGATG GGTACGGCAC CGATTATCGA
     2821 TGACGAGAGC AAGGAAATTC TGGTCAACGC CACCATGGGT GCACCTATCA CGGCCATTAA
     2881 GTTTTGGATC ATTCCTATTA CGGGCTACCA AGGCTCTGTG CTGCCGGCCC TGATCATCGG
     2941 TATCGTCGTT GCATACCTGG AGAAATGGAT TAAGAGCTGG ATGCCGAAAT CTGTGAACAT
     3001 GATCTTCACC CCGTTCCTGA CCATTAGCTT CGCTTTGCTG ATCGCGCTGT TCTTCTTCGG
     3061 TCCGATTCTG TTGATGGTGG AGCAGGGTAT CCTGATTGCA ACCAAGGCAA TCCTGGGCAT
     3121 TAAATACGGT TTCGGTACCG CAATTATCGC TGGTCTGCTC CAAGGTATTG TGATCACCGG
     3181 CTGTCATCAG GTTCTCCAGG GCCTGGAAAT GCAACTGGTG ATTGACGGTT CCCTGCCGGG
     3241 TGCTAATGGC GCTGACCCAG CGGGCAGCAT CTTCAATGCA ATCTGGACGG CGAGCATTAT
     3301 TAGCCAAGGC GGCGCTGCAA TGGCGATCGC GATCAAGGCA AAAGACAAGT CCGATAAAGA
     3361 GCTGGGCTTT AGCGCGGCGT TGAGCACTTT TTTCGGTATC ACCGAACCGG CTATTTTCGG
     3421 TACCAATCTG CCGAAGGTCA AACCGTTTAT TTATGCTAGC ATCGGCGCTT TCTTTGGTGG
     3481 TTTGTTCGCC GGTGCGCTGA ACGTGACCTG TCCTGGCATG GGTGTGACCG TTGTGCCGGG
     3541 TCTGCTGCTG TACACCGGTG ACTGGGTGAA GCTGCTGGGT ATTATTGGCG TTAACATTAT
     3601 TGCGTTCTCG AGCGCCTTTC TGTTGACGTT CTTTCTGTTC AAGGAAGGCG TTGAGGCGAA
     3661 GAAAACCAAA TTAGGTTTGA AGTTCGATGA ATTAAAGGCA AAAGTCAAGT TCGATAAGAT
     3721 TAAATTTCGT AAGAATAAGG CAGAGTGAAG AGCTTAGAGA CCCACTCGAT AGGTACAACC
     3781 GG
//