LOCUS       Exported     3785 bp     DNA     circular     SYN     24-JUL-2020
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Keoni Gandall
  TITLE     Direct Submission
  JOURNAL   Exported from Poly.
FEATURES             Location/Qualifiers
     terminator      1..3785
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA 
 polymerase"
     terminator      38..85
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     primer_bind     1933..1949
                     /label="M13 for"
                     /note="common sequencing primer, one of multiple similar variants"
     terminator      1950..1981
                     /label="tonB terminator"
                     /note="bidirectional E. coli tonB-P14 transcription terminator"
     CDS             2030..3751
                     /label="Mfl519"
                     /note="General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr)."
                     /translation="MSKQFKGIGASEGIAVAKALVLAEDHIEIKKTKVSDIEAEVSKLENAVNKSIEDLENLKATTLEKLGPEKAAIFDAHKEIASDPAIKDEIINVIKSESICAEFAAEQVTNNFYEMFASMDDPYFKERSADIKDVASRIIKHILGIAIVDLSTISEEVIIVAEDLTPSQTAQLNKQFVKGFATNIGGRTSHAAIMARSLEIPAVLGLRTITSDVKNGEVFALNGTTGIVELDLTDTIKSEYEKLAKEFSDLKAELQKFKDLPSKTADGQEKLIEANIGSPTDVESVIEHGGEGIGLFRSEFLYMDNDHFPTEEEQYIAYKQVMDAMKGKLVVIRTLDIGGDKKLSYFEFPHEMNPFLGYRAVRFTLDRKDIFKDQLRALLRASAHGELGIMFPMIATVDEFKRAKAIVEECKEELRKENIAFDENVQVGMMVEIPAAAVNADKFSKYADFFSIGTNDLIQYSMAADRMSENVSYLYQPLNPAILKLIDLTIKGAHKNNKWVGMCGEMAGDIQALPLLLGMGLDAFSMSATSMLRARALMSKITMAEAEELANKALSSDDTPEVIELVDAFLATK"
     primer_bind     complement(90..106)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar variants"
     rep_origin      complement(173..761)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(952..1812)
                     /label="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(1813..1917)
                     /label="bla promoter"
                     /note="ampicillin resistance gene promoter"
ORIGIN
        1 CGCTGAGGTG TCAATCGTCG GAGCCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC
       61 TAAACGGGTC TTGAGGGGTT TTTTGCATGG TCATAGCTGT TTCCTGAGAG CTTGGCAGGT
      121 GATGACACAC ATTAACAAAT TTCGTGAGGA GTCTCCAGAA GAATGCCATT AATTTCCATA
      181 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
      241 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
      301 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
      361 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
      421 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
      481 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
      541 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
      601 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
      661 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
      721 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGGCCTA CTATTAGCAA
      781 CAACGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
      841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
      901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
      961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1021 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1081 AATGATACCG CGAGAACCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1141 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     1201 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     1261 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     1321 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     1381 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     1441 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     1501 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     1561 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     1621 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     1681 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     1741 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     1801 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     1861 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     1921 CTGCACCAGT CAGTAAAACG ACGGCCAGTA GTCAAAAGCC TCCGACCGGA GGCTTTTGAC
     1981 TTGGTTCAGG TGGAGTGGGA GAAACACGTG GCAAACATTC CGGTCTCAAA TGAGCAAACA
     2041 ATTCAAGGGT ATTGGTGCAA GCGAAGGTAT CGCCGTTGCG AAAGCCCTGG TGCTGGCCGA
     2101 GGACCATATT GAAATCAAAA AGACCAAGGT GTCTGATATC GAGGCGGAGG TCAGCAAGCT
     2161 GGAGAATGCC GTTAATAAGT CGATTGAAGA TCTGGAGAAC CTTAAAGCAA CCACCCTGGA
     2221 GAAACTGGGT CCGGAGAAGG CCGCAATCTT TGATGCGCAT AAGGAGATCG CCAGCGATCC
     2281 GGCAATTAAG GACGAGATTA TTAACGTCAT TAAAAGCGAA AGCATTTGCG CGGAGTTCGC
     2341 AGCAGAACAA GTTACGAACA ACTTCTACGA GATGTTCGCG TCCATGGACG ATCCGTATTT
     2401 TAAGGAACGC AGCGCTGACA TTAAGGACGT GGCTTCACGC ATTATCAAGC ATATCTTGGG
     2461 TATCGCTATC GTTGATCTGA GCACCATTAG CGAAGAAGTG ATCATCGTTG CTGAGGACCT
     2521 GACGCCGAGC CAAACCGCTC AACTGAATAA ACAGTTTGTT AAGGGCTTTG CAACCAATAT
     2581 CGGCGGCCGT ACCAGCCATG CAGCCATTAT GGCGAGAAGC CTCGAGATCC CGGCCGTGCT
     2641 GGGTCTGCGT ACGATCACGA GCGACGTGAA AAATGGCGAA GTTTTTGCAC TGAACGGTAC
     2701 GACTGGTATT GTTGAGCTGG ATCTGACCGA CACCATCAAA AGCGAGTACG AAAAGCTGGC
     2761 GAAGGAATTC AGCGATCTGA AGGCGGAGTT GCAAAAATTC AAGGATTTGC CGTCTAAAAC
     2821 CGCAGATGGT CAAGAAAAGC TGATCGAGGC GAACATCGGT TCCCCGACGG ACGTTGAGAG
     2881 CGTCATCGAG CACGGTGGCG AGGGTATTGG TCTGTTCCGT TCGGAGTTCC TGTACATGGA
     2941 CAATGACCAT TTCCCGACCG AAGAGGAGCA ATATATCGCG TATAAACAGG TTATGGACGC
     3001 AATGAAGGGT AAACTGGTTG TTATTCGTAC CCTGGACATC GGCGGCGATA AAAAGCTGTC
     3061 ATATTTTGAG TTCCCGCACG AGATGAATCC GTTCCTGGGT TACCGCGCAG TGCGTTTCAC
     3121 TCTGGATCGC AAAGACATTT TCAAGGACCA ACTGCGCGCG CTGCTGCGTG CGAGCGCGCA
     3181 TGGTGAATTG GGCATTATGT TCCCGATGAT TGCGACCGTC GACGAATTCA AGCGTGCGAA
     3241 AGCCATTGTT GAGGAGTGTA AAGAGGAACT GCGCAAAGAG AATATCGCGT TCGATGAAAA
     3301 TGTTCAAGTC GGTATGATGG TGGAAATCCC GGCCGCTGCG GTGAACGCTG ACAAATTCTC
     3361 CAAGTACGCT GACTTTTTTA GCATCGGTAC CAACGACCTG ATCCAGTACA GCATGGCGGC
     3421 CGATCGTATG TCCGAGAACG TTTCGTATCT GTATCAACCG CTGAATCCTG CAATTCTGAA
     3481 GCTGATCGAT CTGACCATCA AGGGCGCACA CAAAAATAAC AAATGGGTCG GTATGTGCGG
     3541 TGAAATGGCG GGTGATATCC AGGCGCTGCC GCTGTTGTTG GGTATGGGCC TGGACGCATT
     3601 TTCGATGTCC GCAACCAGCA TGCTGCGCGC ACGTGCTCTG ATGAGCAAAA TTACCATGGC
     3661 GGAGGCGGAG GAACTGGCGA ACAAAGCGCT GAGCTCTGAT GATACGCCTG AGGTCATCGA
     3721 GCTGGTCGAC GCGTTCTTGG CAACCAAGTG AAGAGCTTAG AGACCCAACC TGGTATGCAG
     3781 TCCTT
//